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بررسی خصوصیات بیولوژیکی و مولکولی چند جدایه ویروس موزائیک خیارCucumber mosaic virus (CMV))) از مناطق شمال غرب ایران | ||
| پژوهش های کاربردی در گیاهپزشکی | ||
| مقاله 8، دوره 6، شماره 3، پاییز 1396، صفحه 97-108 اصل مقاله (789.87 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| نویسندگان | ||
سویل نعمت اللهی  1؛ نعمت سخندان2؛ فرشاد رخشنده رو3؛ حمیدرضا زمانی زاده3
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| 1استادیار، گروه گیاه پزشکی، دانشگاه آزاد اسلامی، واحد تبریز، تبریز. | ||
| 2دانشیار، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز. | ||
| 3استادیار گروه گیاه پزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی ،تهران. | ||
| چکیده | ||
| چکیده ویروس موزائیک خیار یکی از مهمترین ویروسهای آلوده کنندهی گیاهی در ایران میباشد. به منظور بررسی فنوتیپ آلودگی جدایههای مختلف این ویروس در سال 1390 تعداد 342 نمونهی گیاهی مشکوک به آلودگی با این ویروس از گیاهان خیار، کدو، خربزه و گوجه فرنگی از مناطق مختلف شمال غرب کشور جمعآوری و با آزمون الایزا 91 نمونهی آلوده به CMV ردیابی شد. از بین جدایه ها هشت جدایه که توالی ژنومی و موقعیت تبارزایی آنها مشخص گردیده بود، برای مقایسهی خصوصیات بیولوژیکی و مطالعات بیشتر انتخاب شدند. شاخص بیماری برای این جدایه ها روی کدو محاسبه گردید. براساس علایم، جدایه ها در دو گروه متمایز شدند. بررسی بیان ژن پروتئین پوششی و سرکوب گر خاموشی RNA ((2b با استفاده از تکنیک RT-PCR نیمه کمی، نشان داد که غلظت جدایهها در گیاهان محک متفاوت میباشد. جدایههای ایرانی زیر گروه IB علائم شدیدتری را بر روی گیاهان محک نسبت به جدایه های زیر گروه IA نشان دادند. بنابراین به نظر میرسد که بین ویژگیهای ژنوتیپی و خصوصیات بیماری زایی جدایه ها ارتباط معنی داری وجود دارد. | ||
| کلیدواژه‌ها | ||
| واژه های کلیدی: ویروس موزائیک خیار؛ خصوصیات بیولوژیک؛ تبار زایی؛ زیر گروه | ||
| عنوان مقاله [English] | ||
| Molecular and Biological Characteristics of Some Iranian Isolates of Cucumber mosaic virus from North West Region of Iran | ||
| نویسندگان [English] | ||
| sevil nematollahi1؛ nemat sokhandan2؛ farshad rakhshandeh3؛ hamidreza zamanizadeh3 | ||
| 1Assistant Professor, Department of Plant Protection, Tabriz Branch, Islamic Azad University, Tabriz, Iran. | ||
| 2Associate Professor, Department of Plant Protection, Faculty of Agriculture University of Tabriz, | ||
| 3Assistant Professor, Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده [English] | ||
| Abstract Cucumber mosaic virus (CMV) is one of the most important infectious plant viruses in Iran. For evaluating infection phenotypes of different isolates 342 samples of cucurbitaceous and tomato plants were collected from different locations in the northwest region of Iran in 2012. After screening by enzyme-linked immune sorbent assay (ELISA), 91 CMV-infected samples were identified. Eight representative CMV isolates whose phylogenetic positions had been determined were selected and compared for their pathological features in zucchini (Cucurbita pepo cv. Ps) plants. According to the determined disease indexes, the isolates were placed in two main groups. Also, evaluation of 2b (viral suppressor of RNA silencing) and CP expression with semi-quantitative RT-PCR showed different concentration of the isolates in test plants. The subgroup IB members from Iran induced more severe symptoms on the inoculated indicator test plants compared to the isolates in subgroup IA. Thus, there seemed to be a correlation between subgroup affiliation and biological properties of the CMV isolates from Iran. | ||
| کلیدواژه‌ها [English] | ||
| Keywords: CMV, Phylogenetic, Subgroup, Phenotype | ||
| مراجع | ||
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جعفری م، شمس بخش م و معینی ا، 1395. واﮐﻨﺶ ارﻗﺎم ﺗﺠﺎری و ﻻﯾﻦﻫﺎی ﮐﻠﺰا ﺑﻪ ﺟﺪاﯾﻪ وﯾﺮوس موزاییک شلغم، بیماریهای گیاهی، جلد 52، شماره 2 : 159-147. Bananej K and Vahdat A, 2008. Identification, distribution and incidence of viruses in field-grown cucurbit crops of Iran. Phytopathologia Mediterranea 7: 247–257. Bashir NS, Kolhar MR and Zarghani SN, 2006. Detection, differentiation and phylogenetic analysis of cucumber mosaic virus isolates from cucurbits in the northwest region of Iran. Virus Genes 32:277-288. Bashir NS, Nematollahi S and Torabi E, 2008. Cucumber mosaic virus subgroup IA frequently occurs in the northwest IRAN. Acta Virologica 52:237-242. Converse R H and Martin RR, 1990. ELISA methods for plant viruses. Pp. 389 . In: R., Hampton, E., Ball and S. De Boer (eds.). Serological Methods for Detection and Identification of Viral and Bacterial Plant Pathogens. A laboratory manual. APS Press. Chare ER and Holmes E C. 2005, A phylogenetic survey of recombination frequency in plant RNA viruses.Archives of Virology 151:933-946. Chaerani R and Voorrips RE, 2006. Tomato early blight (Alternaria solani): the pathogen, genetics, and breeding for resistance. Journal of General Plant Pathology 72:335–347. Choi SK, Palukatis P, Min BE, Lee MY, Choi JK and Ryu KH, 2005. Cucumber mosaic virus 2a polymerase and movement proteins independently affect both virus movement and the timing of symptom development in zucchini squash. Journal of General Virology 86:1213-1222. Ding SW, Anderson Bj, Haase HR and Symons RH, 1994. New overlapping gene encoded by the cucumber mosaic virus genome .Virology198:593-601. Ding SW, Shi BJ, Li WX and Symons RH, 1996. An interspecies hybrid RNA virus is significantly more virulent than either parental virus. Proceeding of the National Academy of Sciences of the United States of America 93:7470-7474. Du ZY, Chen FF, Liao QS, Zhang HR, Chen YF and Chen JS, 2007. 2b ORFs encoded by subgroup IB strains of cucumber mosaic virus induce differential virulence on Nicotiana species. Journal of General Virology 88: 2596-2604. Hayes RJ and Buck KW, 1990. Complete replication of a eukaryoticvirus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363-368. Lewsey M, Robertson FC, Canto T, Palukaitis, P and Carr JP, 2007. Selective targeting of miRNA-regulated plant development bya viral counter-silencing protein. Plant Journal 50: 240–252. Massumi H, Shaabanian M, Hosseini Pour A, Heydarnejad, J and Rahimian, H, 2009. Incidence of viruses infecting tomato and their natural hosts in the southeast and central regions of Iran. Plant Disease 93:67-72. Mochizuki T, Yamazaki R, Wada T and Ohki ST, 2014. Coat protein mutations in an attenuated Cucumber mosaic virus encoding mutant 2b protein that lacks RNA silencing suppressor activity induces chlorosis with photosynthesis gene repression and chloroplast abnormalities in infected tobacco plants. Virology 456:292-299.Nematollahi S, Sokhandan-Bashir N, Rakhshandehroo F and Zamanizadeh H, 2012. Phylogenetic Analysis of New Isolates of Cucumber mosaic virus from Iran on the Basis of Different Genomic Regions. The Plant Pathology Journal 28(4) : 381-389. Nouri S, Arevalo R, Falk BW and Groves RL, 2014. Genetic structure and molecular variability of Cucumber mosaic virus isolates in the United States. PLoS NE 9(5):1-12.Palukaitis P and Garcia-Arenal F, 2003. Cucumoviruses. Advance Virus Research 62: 241-323. Phan MS, Seo J, Choi HS, Lee SH and Kim KH, 2014. Pseudore combination between two distinct strains of Cucumber mosaic virus results in enhancement of symptom sseverity. The Plant Pathology Journal 30:316-322. Rakhshandehroo F, Takeshita M, Squires J and Palukaitis P, 2009. The Influence of RNA-Dependent RNA Polymerase 1on Potato virus Y Infection and on Other Antiviral Response Genes. Molecular Plant Microbe Interaction 22: 1312–1318. Rasoulpour R and Izadpanah K, 2008. Proporties and taxonomic position of hoary cress strain of Cucumber mosaic virus. Journal of Plant Pathology 90 (1): 97-102. Rizos H, Gunn LV, Pares RD and Gillings RM,1992. Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction. Journal of General Virology 73:2099-2103. Roossinck M, Zhang JL and Hellwald H, 1999. Rearrangements in the 5' nontranslated region and phylogenetic analyzes of cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. Journal of Virology73:6752–6758. Roossinck M J, 2002. Evolutionary history of Cucumber mosaic virus deduced by phylogenetic analyses. Journal of Virology 76:3382-3387. Rowhani A, Chay c, Golino DA and Falk W, 1993. Development of a polymerase chain reaction technique for the detection of Grapevine fanleaf virus in a grapevine tissue. Phytopathology83: 749-753. Schneider WL and Roossinck MJ. 2001. Genetic diversity in RNA virus quasispecies is controlled by host-virus interactions. Journal of Virology 75:6566-6571. Shi BJ, Palukaitis P and Symons RH, 2002. Differential virulence by strains of Cucumber mosaic virus is mediated by the 2b gene. Molecular Plant-Microbe Interaction 15: 947–955.
Soards AJ, Murphy AM, Palukaitis P and Carr PJ, 2002.Virulence and differential local and systemic spread of cucumber mosaic virus in tobacco are affected by the CMV 2b protein. Molecular Plant-Microbe Interaction 15:647–653.
Strausbaugh C. A., Myers, J. R., Forster R. L., and McClean P. E, 2003. A quantitative method to screen common bean plants for resistance to Bean common mosaic necrosis virus . Phytopathology 93:1430–6.
Takeshita M, Matsuo Y, Suzuki M, Furya N, Tsuchiya K and Takanami Y, 2009. Impact of a defective RNA3 from cucumber mosaic virus on helper virus infection dynamics. Vitology 389:59-65.
Wahuny WS, Dietzgen RG, Hanada K and Francki RIB, 1992. Serological and biological variation between and within subgroub I and П strains of cucumber mosaic virus. Plant Pathology 41:282-297.
Wang Y, Tzfira T, Gaba V, Citovsky V, Palukaitis P and Gal-On A, 2004. Functional analysis of the Cucumber mosaic virus 2b protein: pathogenicity and nuclear localization. Journal of General Virology 85:3135-3147.
Zeng R, Liao Q, Feng J, Li D and Chen J, 2007. Synergy between Cucumber Mosaic Virus and Zucchini Yellow Mosaic Virus on Cucurbitaceae hosts tested by Real-time Reverse Transcription-Polymerase chain reaction. Acta Biochimica et Biophysica Sinica 39(6): 431-437.
Zhang X, Yuan YR, Pei Y, Lin SS, Tuschl T, Patel, DJ and Chua NH, 2006. Cucumber mosaic zvirus-encoded 2b suppressorinhibits Arabidopsis Argonaute1 cleavage activity to counter plantdefense. Genes Development 20: 3255–3268
Ziebell H, Payne T, Berry J O, Walsh JA and Carr JP, 2007. A cucumber mosaic virus mutant lacking the 2b counter-defence protein gene provides protection against wild-type strains. Journal of General Virology 88: 2862–2871. | ||
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