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بررسی خصوصیات بیولوژیکی و مولکولی چند جدایه ویروس موزائیک خیارCucumber mosaic virus (CMV))) از مناطق شمال غرب ایران | ||
| پژوهش های کاربردی در گیاهپزشکی | ||
| مقاله 8، دوره 6، شماره 3، پاییز 1396، صفحه 97-108 اصل مقاله (789.87 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| نویسندگان | ||
سویل نعمت اللهی  1؛ نعمت سخندان2؛ فرشاد رخشنده رو3؛ حمیدرضا زمانی زاده3
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| 1استادیار، گروه گیاه پزشکی، دانشگاه آزاد اسلامی، واحد تبریز، تبریز. | ||
| 2دانشیار، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز. | ||
| 3استادیار گروه گیاه پزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی ،تهران. | ||
| چکیده | ||
| چکیده ویروس موزائیک خیار یکی از مهمترین ویروسهای آلوده کنندهی گیاهی در ایران میباشد. به منظور بررسی فنوتیپ آلودگی جدایههای مختلف این ویروس در سال 1390 تعداد 342 نمونهی گیاهی مشکوک به آلودگی با این ویروس از گیاهان خیار، کدو، خربزه و گوجه فرنگی از مناطق مختلف شمال غرب کشور جمعآوری و با آزمون الایزا 91 نمونهی آلوده به CMV ردیابی شد. از بین جدایه ها هشت جدایه که توالی ژنومی و موقعیت تبارزایی آنها مشخص گردیده بود، برای مقایسهی خصوصیات بیولوژیکی و مطالعات بیشتر انتخاب شدند. شاخص بیماری برای این جدایه ها روی کدو محاسبه گردید. براساس علایم، جدایه ها در دو گروه متمایز شدند. بررسی بیان ژن پروتئین پوششی و سرکوب گر خاموشی RNA ((2b با استفاده از تکنیک RT-PCR نیمه کمی، نشان داد که غلظت جدایهها در گیاهان محک متفاوت میباشد. جدایههای ایرانی زیر گروه IB علائم شدیدتری را بر روی گیاهان محک نسبت به جدایه های زیر گروه IA نشان دادند. بنابراین به نظر میرسد که بین ویژگیهای ژنوتیپی و خصوصیات بیماری زایی جدایه ها ارتباط معنی داری وجود دارد. | ||
| کلیدواژه‌ها | ||
| واژه های کلیدی: ویروس موزائیک خیار؛ خصوصیات بیولوژیک؛ تبار زایی؛ زیر گروه | ||
| عنوان مقاله [English] | ||
| Molecular and Biological Characteristics of Some Iranian Isolates of Cucumber mosaic virus from North West Region of Iran | ||
| نویسندگان [English] | ||
| sevil nematollahi1؛ nemat sokhandan2؛ farshad rakhshandeh3؛ hamidreza zamanizadeh3 | ||
| 1Assistant Professor, Department of Plant Protection, Tabriz Branch, Islamic Azad University, Tabriz, Iran. | ||
| 2Associate Professor, Department of Plant Protection, Faculty of Agriculture University of Tabriz, | ||
| 3Assistant Professor, Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده [English] | ||
| Abstract Cucumber mosaic virus (CMV) is one of the most important infectious plant viruses in Iran. For evaluating infection phenotypes of different isolates 342 samples of cucurbitaceous and tomato plants were collected from different locations in the northwest region of Iran in 2012. After screening by enzyme-linked immune sorbent assay (ELISA), 91 CMV-infected samples were identified. Eight representative CMV isolates whose phylogenetic positions had been determined were selected and compared for their pathological features in zucchini (Cucurbita pepo cv. Ps) plants. According to the determined disease indexes, the isolates were placed in two main groups. Also, evaluation of 2b (viral suppressor of RNA silencing) and CP expression with semi-quantitative RT-PCR showed different concentration of the isolates in test plants. The subgroup IB members from Iran induced more severe symptoms on the inoculated indicator test plants compared to the isolates in subgroup IA. Thus, there seemed to be a correlation between subgroup affiliation and biological properties of the CMV isolates from Iran. | ||
| کلیدواژه‌ها [English] | ||
| Keywords: CMV, Phylogenetic, Subgroup, Phenotype | ||
| مراجع | ||
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