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شناسایی گونه های Fusarium همراه علایم پوسیدگی ریشه کلزا در استان خوزستان | ||
پژوهش های کاربردی در گیاهپزشکی | ||
دوره 10، شماره 1، فروردین 1400، صفحه 29-43 اصل مقاله (2.47 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22034/arpp.2021.12465 | ||
نویسندگان | ||
عذرا نعمتی مندنی پور1؛ رضا فرخی نژاد1؛ مهدی مهرابی کوشکی* 2 | ||
1گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران. | ||
2گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران. مرکز تحقیقات بیوتکنولوژی و علوم زیستی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
چکیده | ||
چکیده گیاه کلزا میزبان تعداد زیادی عوامل قارچی بیماریزا و غیربیماریزا از جمله گونه های جنس Fusarium است. در طول سال های ۹۸-۹۶، 40 نمونه گیاهی کلزا با علایم زردی، پژمردگی و پوسیدگی ریشه در استان خوزستان جمعآوری گردید، از این نمونه ها، 35 جدایه Fusarium روی محیط کشت سیب زمینی دکستروز-آگار (PDA) بدست آمد. ده جدایه از آنها بر اساس شکل پرگنه و کنیدیوم ها انتخاب و بر اساس تجزیه و تحلیل تبارزایی و ریخت شناسی شناسایی شدند. برای بررسی مولکولی، دو ناحیه ITS دی ان ای ریبوزومی (ITS) و ژن کد کننده فاکتور تداوم ترجمه (tef1) توالی یابی و در جستجوی بلاست و تجزیه و تحلیل تبارزایی با استفاده از الگوریتم درست نمایی بیشینه و استنباط بیژین استفاده شدند. بر اساس تلفیق داده های ریخت شناختی و داده های مولکولی، در این تحقیق گونه های Fusarium acuminatum، F. clavum، F. gracilipes، F. incarnatum و F. tanahbumbuense و یک گونه نماینده دودمان جدید شناسایی شدند. بر اساس دانش ما، این اولین گزارش گونه های F. acuminatum، F. clavum، F. gracilipes، F. incarnatum و F. tanahbumbuense روی کلزا در ایران است. همچنین گونه F. gracilipes برای میکوبیوتای ایران جدید است. | ||
کلیدواژهها | ||
کلمات کلیدی: کمپلکس FIESC؛ ریخت شناسی؛ تبارزایی مولکولی؛ دودمان | ||
مراجع | ||
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