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مهار بیماری پوسیدگی نرم سیبزمینی با کاربرد سیلیکون، نانو ذرات سیلیکون و باکتری آنتاگونیست Bacillus methylotrophicus در شرایط گلخانه ای | ||
پژوهش های کاربردی در گیاهپزشکی | ||
دوره 10، شماره 1، فروردین 1400، صفحه 83-96 اصل مقاله (1.33 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22034/arpp.2021.12442 | ||
نویسندگان | ||
علی ویانی* 1؛ پروانه جوراب باف1؛ ناصر علی اصغرزاد2 | ||
1گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز. | ||
2گروه علوم خاک دانشکده کشاورزی، دانشگاه تبریز. | ||
چکیده | ||
چکیده در این پژوهش تاثیر باکتری آنتاگونیست Bacillus methylotrophicus در مهار زیستی باکتری Pectobacterium carotovorum subsp. carotovorumعامل بیماری پوسیدگی نرم سیب زمینی و همچنین تاثیر مواد القا کننده مقاومت سیستمیک اکتسابی شامل سیلیکون و نانوذرات سیلیکون بر هر دو باکتری در شرایط آزمایشگاهی و گلخانه ای مورد بررسی قرار گرفت. در آزمونهای حلقههای کاغذی و نشت در چاهک، سیلیکون و نانوذرات سیلیکون هیچ نوع هاله بازداری علیه این دو باکتری ایجاد نکردند اما باکتری آنتاگونیست B. methylotrophicus در هر دو آزمون و همچنین آزمون کلروفرم توانست هاله بازداری از رشد باکتری بیمارگر به ترتیب به شعاع 9، 5/8 و 15میلیمتر را ایجاد کند. پس از گذشت 6 و 10 ساعت از کشت در محیط مایع، نانوذرات سیلیکون، جمعیت هر دو باکتری بیمارگر و آنتاگونیست را اندکی کاهش داد اما پس از گذشت 24 ساعت، تفاوت آنها با شاهد معنیدار نبود. سیلیکون در محیط کشت مایع نتوانست از رشد هیچکدام از باکتریها ممانعت کند. در شرایط گلخانه ای، تیمارهای سیلیکون و نانو ذرات سیلیکون و نیز باکتری آنتاگونیست، وزن تر و خشک ریشه و اندام های هوایی را در مقایسه با تیمار شاهد مثبت به طور معنی داری افزایش دادند و بیشترین تاثیر، درتیمارهای ترکیبی سیلیکون یا نانوذرات سیلیکون با باکتری آنتاگونیست مشاهده شد. تمامی تیمارها در مقایسه با شاهد آلوده توانستند پوسیدگی نرم را در گیاهان سیب زمینی بهطور کامل مهار کنند و هیچ علائمی از بیماری در آنها مشاهده نگردید، در حالیکه در تیمارهای شاهد، تمامی غدههای کشت شده، نشانههای لهیدگی و پوسیدگی را بروز داده و از بین رفتند. | ||
کلیدواژهها | ||
کلمات کلیدی: پوسیدگی نرم؛ مواد القا کننده مقاومت؛ مقاومت القایی؛ مقاومت سیستمیک اکتسابی؛ مهار زیستی | ||
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