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مهار بیماری پوسیدگی نرم سیبزمینی با کاربرد سیلیکون، نانو ذرات سیلیکون و باکتری آنتاگونیست Bacillus methylotrophicus در شرایط گلخانه ای | ||
پژوهش های کاربردی در گیاهپزشکی | ||
دوره 10، شماره 1، فروردین 1400، صفحه 83-96 اصل مقاله (1.33 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22034/arpp.2021.12442 | ||
نویسندگان | ||
علی ویانی* 1؛ پروانه جوراب باف1؛ ناصر علی اصغرزاد2 | ||
1گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز. | ||
2گروه علوم خاک دانشکده کشاورزی، دانشگاه تبریز. | ||
چکیده | ||
چکیده در این پژوهش تاثیر باکتری آنتاگونیست Bacillus methylotrophicus در مهار زیستی باکتری Pectobacterium carotovorum subsp. carotovorumعامل بیماری پوسیدگی نرم سیب زمینی و همچنین تاثیر مواد القا کننده مقاومت سیستمیک اکتسابی شامل سیلیکون و نانوذرات سیلیکون بر هر دو باکتری در شرایط آزمایشگاهی و گلخانه ای مورد بررسی قرار گرفت. در آزمونهای حلقههای کاغذی و نشت در چاهک، سیلیکون و نانوذرات سیلیکون هیچ نوع هاله بازداری علیه این دو باکتری ایجاد نکردند اما باکتری آنتاگونیست B. methylotrophicus در هر دو آزمون و همچنین آزمون کلروفرم توانست هاله بازداری از رشد باکتری بیمارگر به ترتیب به شعاع 9، 5/8 و 15میلیمتر را ایجاد کند. پس از گذشت 6 و 10 ساعت از کشت در محیط مایع، نانوذرات سیلیکون، جمعیت هر دو باکتری بیمارگر و آنتاگونیست را اندکی کاهش داد اما پس از گذشت 24 ساعت، تفاوت آنها با شاهد معنیدار نبود. سیلیکون در محیط کشت مایع نتوانست از رشد هیچکدام از باکتریها ممانعت کند. در شرایط گلخانه ای، تیمارهای سیلیکون و نانو ذرات سیلیکون و نیز باکتری آنتاگونیست، وزن تر و خشک ریشه و اندام های هوایی را در مقایسه با تیمار شاهد مثبت به طور معنی داری افزایش دادند و بیشترین تاثیر، درتیمارهای ترکیبی سیلیکون یا نانوذرات سیلیکون با باکتری آنتاگونیست مشاهده شد. تمامی تیمارها در مقایسه با شاهد آلوده توانستند پوسیدگی نرم را در گیاهان سیب زمینی بهطور کامل مهار کنند و هیچ علائمی از بیماری در آنها مشاهده نگردید، در حالیکه در تیمارهای شاهد، تمامی غدههای کشت شده، نشانههای لهیدگی و پوسیدگی را بروز داده و از بین رفتند. | ||
کلیدواژهها | ||
کلمات کلیدی: پوسیدگی نرم؛ مواد القا کننده مقاومت؛ مقاومت القایی؛ مقاومت سیستمیک اکتسابی؛ مهار زیستی | ||
مراجع | ||
Ahmadi K, Ebadzadeh HR, Hatami F, Abdshah H, Kazemian A, 2019. Agricultural Statistical Report of Cropping Year 2017-2018. Volume 1: crop production. Ministry of Agriculture-jahad, Planning and Economic Affairs, Communication and Information Technology Center. 87 pp. Azadmanesh S, Mozafari J, Hasanzadeh N, Moslemkhani C, 2017. In vitro evaluation of potato genotypes for resistance against bacterial soft rot (Pectobacterium carotovorum) – a new tool for studying disease resistance. Journal of Plant Protection Research 57 (1): 1–8 (in Persian with English abstract). Azaiez S, Ben Slimene I, Karkouch I, Essid R, Jallouli S, et al.,2018. Biological control of the soft rot bacterium Pectobacterium carotovorum by Bacillus amyloliquefaciens strain Ar10 producing glycolipid-like compounds. Microbiological Research 217: 22–23. Balouiri M, Sadiki M, Ibnsouda SK, 2016. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis 6: 71–79. Bhat KA, Viswanath HS, Bhat NA, Wani TA, 2017. Bioactivity of various ethanolic plant extracts against Pectobacterium carotovorum subsp. carotovorum causing soft rot of potato tubers. Indian Phytopathology 70 (4): 463–470. Cheng X, Ji X, Ge Y, Li J, Qi W, et al., 2019. Characterization of antagonistic Bacillus methylotrophicus isolated from rhizosphere and its biocontrol effects on maize stalk rot. Phytopathology 109 (4): 571–581. Chung S, Kong H, Buyer JS, Lakshman DK, Lydon J, et al., 2008. Isolation and partial characterization of Bacillus subtilis ME488 for suppression of soilborne pathogens of cucumber and pepper. Applied Microbiology and Biotechnology 80: 115–123. Cui J, Liu T, Li F, Yi J, Liu C, et al., 2017. Silica nanoparticles alleviate cadmium toxicity in rice cells: mechanisms and size effects. Environmental Pollution 228: 363–369. Diogo RV, Wydra K, 2007. Silicon-induced basal resistance in tomato against Ralstonia solanacearum is related to modification of pectic cell wall polysaccharide structure. Physiological and Molecular Plant Pathology 70: 120–129. FAO, 2018. FAOSTAT/ production / crops, http://www.fao.org/faostat/en/#data/QC Ge B, Liu B, Nwet TT, Zhao W, Shi L, et al., 2016. Bacillus methylotrophicus Strain NKG-1, Isolated from Changbai mountain, China, has potential applications as a biofertilizer or biocontrol agent. PLOS ONE 11 (11): e0166079. Gerayeli N, Baghaee-Ravari S, Tarighi S, 2017. Evaluation of the antagonistic potential of Bacillus strains against Pectobacterium carotovorum subsp. carotovorum and their role in the induction of resistance to potato soft rot infection. European Journal of Plant Pathology150: 1049–1063. Gillman JH, Zlesak DC, Smith JA, 2003. Applications of potassium silicate decrease black spot infection in Rosa hybrida Meipelta (Fuschia Meidiland™). Horticulture Science 38: 1144–1147. Gomaa EZ, 2013. Antimicrobial activity of a biosurfactant produced by Bacillus licheniformis strain M104 grown on whey. Brazilian Archives of Biology and Technology 56: 259–268. Gu Q, Yang Y, Yuan Q, Shi G, Wu L, et al. 2017. Bacillomycin D produced by Bacillus amyloliquefaciens is involved in the antagonistic interaction with the plant pathogenic fungus Fusarium graminearum. Applied Environmental Microbiology 83 (19): e01075–17. Haghighi M, Afifipour Z, Mozafarian M, 2012. The effect of N–Si on tomato seed germination under salinity levels. Journal of Biological and Environmental Sciences (16): 87–90. Haghighi M, Pessarakli M, 2013. Influence of silicon and nano-silicon on salinity tolerance of cherry tomatoes (Solanum lycopersicum L.) at early growth stage. Scientia Horticulturae 16: 111–117. Jemil N, Manresa A, Rabanal F, Ben-Ayed H, Hmidet N, et al., 2017. Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strain. Journal of Chromatography B 1060: 374–386. Jinger D, Devi MT, Dhar Sh, Dass A, Rajanna GA, et al., 2017. Silicon in mitigating biotic stresses in rice (Oryza sativa L.) - a review. Annals of Agricultural Research, New Series 38 (1): 1–14. Joorabbaf P, Viani A, Ghasemi M, 2018. The effect of silicon and silicon nanoparticles on early blight disease of tomato and potato at laboratory and glasshouse conditions. Proceedings of the 23nd Iranian Plant Protection Congress. P. 255 (in Persian with English abstract). Joorabbaf P, Viani A, Parsa N, 2018. Biological control of early blight in tomato and potato and Fusarium wilt of tomato by application of Bacillus methylotrophicus. Proceedings of the 23nd Iranian Plant Protection Congress. P. 257 (in Persian with English abstract). Karimi J, MohsenzadehS, 2016. Effects of silicon oxide nanoparticles on growth and physiology of wheat seedlings. Russian Journal of Plant Physiology 63 (1): 119–123. Karunakaran G, Suriyaprabha R, Manivasakan P, Yuvakkumar R, Rajendran V, et al., 2013. Effect of nanosilica and silicon sources on plant growth promoting rhizobacteria, soil nutrients and maize seed germination. IET Nanobiotechnology 7 (3): 70–77. Khodakaramian DH, Zafari D, 2009. Identification of fluorescent pseudomonads isolated from potato Rhizospher and assessment of their antagonistic activity towards Pectobacterium carotovorum under field condition. Plant Pest and Diseases 77 (1): 2–18. Krzyzanowska DM, Potrykus M, Golanowska M, Polonis K, Gwizdek-Wisniewska A, et al., 2012. Rhizosphere bacteria as potential biocontrol agents against soft rot caused by various Pectobacterium and Dickeya spp. strains. Journal of Plant Pathology 94: 367–378. Li P, Ding W, Liu Q. Wang D, Wang R, et al., 2016a. Mechanism of silicon and BTH-induced resistance to tobacco bacterial wilt. Tobacco Science and Technology 49 (7): 23–30. Li X, Zhang Y, Wei Z, Guan Z, Cai Y, et al., 2016b. Antifungal activity of isolated Bacillus amyloliquefaciens SYBC H47 for the biocontrol of Peach gummosis. PLOS One 11: 1–22. Luyckx M, Hausman JF, Lutts S, Guerriero G, 2017. Silicon and plants: current knowledge and technological perspectives. Frontiers in Plant Science 8: 411. Ning D, Song A, Fan F, Li Z, Liang Y, 2014. Effects of slag-based silicon fertilizer on rice growth and brown-spot resistance. PLOS ONE 9:e102681. O’Farrell N, Houlton A, Horrocks BR, 2006. Silicon nanoparticles: applications in cell biology and medicine. International Journal of Nanomedicine 1 (4): 451–472. Omardien S, Brul S, Zaat SA, 2016. Antimicrobial activity of cationic antimicrobial peptides against gram-positives: Current progress made in understanding the mode of action and the response of bacteria. Frontiers in Cell and Developmental Biology 4: 111. Ongena M, Jacques P, 2007. Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends in Microbiology 16: 115–125. Parsa N, Viani A, Arzanlou M, Aliasgharzad N, 2018. Evaluation of tomato cultivars for resistance to Fusarium wilt disease and influence of mycorhizal fungus Glomus versiforme, antagonistic bacterium Bacillus methylotrophicus and carbendazim fungicide on disease control. MSc thesis, Faculty of Agriculture, University of Tabriz, Iran (in Persian with English abstract). Raaijmakers JM, De Bruijn I, Nybroe O, Ongena M, 2010. Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics. FEMS Microbiology Review 34: 1037–1062. Rahman A, Wallis CM, Uddin W, 2015. Silicon-induced systemic defense responses in perennial ryegrass against infection by Magnaporthe oryzae. Phytopathology 105: 748–757. Rangaraj S, Gopalu K, Rathinam Y, Periasamy P, Venkatachalam R, et al., 2014. Effect of silica nanoparticles on microbial biomass and silica availability in maize rhizosphere. Biotechnology and Applied Biochemistry61 (6): 668–675. Rasipour L, Aliasghar-zad N, 2017. Interaction of phosphate solubilizing bacteria and Bradyrhizobium japanicum on growth index, tuber production and absorption of some nutrients in soybean. Journal of Science and Technology of Agriculture and Natural Resources 11 (40): 53–63. Reignault P, Walters D, 2007. Topical Application of Inducers for Disease Control. In: Walters D, Newton A, Lyon G (eds). Induced resistance for plant defense: A sustainable approach to crop protection. Blackwell Publishing, Ames, IA, Pp. 179–200. Remus-Borel W, Menzies JG, Belanger RR, 2005. Silicon induces antifungal compounds in powdery mildew-infected wheat. Physiological and Molecular Plant Pathology 66: 108–115. Sabbagh SK, Sabbagh E, Abkho J, Zinati Fakhrabad F, 2016. The effect of salicylic acid to induce systemic resistance in cucumber plant to damping-off disease caused by Pythium aphanidermatum. Journal of Applied Research in PlantProtection 5 (2): 27–43 (in Persian with English abstract). Safari E, Sharifi R, Abbassi S, 2020. Inhibition of wheat take-all disease using defenses-inducing compounds. Journal of Applied Research in Plant Protection 9 (3): 1–10 (in Persian with English abstract). Saha ND, Chaudhary A, Singh SD, Singh D, Walia S, et al., 2015. Plant pathogenic microbial communication affected by elevated temperature in Pectobacterium carotovorum subsp. Carotovorum. Current Microbiology 71: 585–593. Shan H, Zhao M, Chen D, Cheng J, Li J, et al., 2013. Biocontrol of rice blast by the phenaminomethylacetic acid producer of Bacillus methylotrophicus strain BC79. Crop Protection 44: 29–37. Shi C, Yan P, Li J, Wu H, Li Q, et al., 2014. Biocontrol of Fusarium graminearum growth and deoxynivalenol production in wheat kernels with bacterial antagonists. International Journal of Environmental Research and Public Health 11: 1094–1105. Strout G, Russell SD, Pulsifer DP, Erten S, Lakhtakia A, et al., 2013. Silica nanoparticles aid in structural leaf coloration in the Malaysian tropical rainforest understorey herb Mapania caudata. Annals of Botany 112 (6): 1141–1148. Sun D, Hussain HI, Yi Z, Rookes JE, Kong L, et al., 2016. Mesoporous silica nanoparticles enhance seedling growth and photosynthesis in wheat and lupin. Chemosphere 152: 81–91. Tavakol Norabadi M, Sahebani N, Etebarian HR, 2014. Management of tomato wilt disease caused by Fusarium oxysporum f. sp. lycopersici with silicon and Pseudomonas fluorescens (CHAO) and assaying activity of Phenylalanine ammonia lyase (PAL). Research in Plant Pathology 2 (3): 13–26 (in Persian with English abstract). Yang H, Li X, Li X, Yu H, Shen Z, 2015. Identification of lipopeptide isoforms by MALDI-TOF-MS/MS based on the simultaneous purification of iturin, fengycin and surfactin by RP-HPLC. Analytical and Bioanalytical Chemistry 407: 2529–2542. Yousefi H, Sahebani N, Mirabolfathy M, Faravardeh L, Mahdavi V, 2011. The effect of salicylic acid and Bacillus subtilis on cucumber root and stem rot, caused by Fusarium oxysporum f. sp. radicis cucumerinum. Iranian Journal of Plant Pathology 46 (4): 85–87. Yuan X, Hou X, Chang H, Yang R, Wang F, et al., 2019. Bacillus methylotrophicus has potential applications against Monilinia fructicola. Open Life Science 14: 410–419. Zhang C, Wang L, Zhang W, Zhang F, 2013. Do lignification and silicification of the cell wall precede silicon deposition in the silica cell of the rice (Oryza sativa L.) leaf epidermis?. Plant Soil 372: 137–149. | ||
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