آمار
| تعداد نشریات | 45 |
| تعداد شمارهها | 1,406 |
| تعداد مقالات | 17,246 |
| تعداد مشاهده مقاله | 55,711,939 |
| تعداد دریافت فایل اصل مقاله | 17,892,804 |
In Vitro Propagation of Lisianthus (Eustomagr andiflurom) | ||
| Journal of Plant Physiology and Breeding | ||
| مقاله 6، دوره 7، شماره 2، اسفند 2017، صفحه 53-64 اصل مقاله (622.52 K) | ||
| نوع مقاله: Research Paper | ||
| نویسندگان | ||
| Rooholah Jafari1؛ Ahmad Moieni* 2؛ Ghasem Karimzadeh2؛ Zahra Movahedi3 | ||
| 1MSc, Department of Agricultural Biotechnology, Tarbiat Modares University, Tehran, Iran | ||
| 2Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 3Department of Agronomy and Plant Breeding, Faculty of Agriculture, Malayer University, Malayer, Iran | ||
| چکیده | ||
| Nowadays, the most rapid method for producing healthy and disease-free Lisiantus is micropropagation. With respect to high economic value of this plant which is regarded among the 10 top cutting flowers in the world, this research was carried out to suggest a suitable protocol for its in vitro propagation, using nodal sections as an explant. To carry out this object, the effects of the pH (5.5, 5.6, 5.7, 5.8), culture vessel (small glass bottle, large glass bottle, polypropylene container), the concentration of macro elements, including NH4NO3 (1.45, 1.65, 1.85 g L-1), KNO3 (1.7, 1.9, 2.1 g L-1), CaCl2.2H2O (0.66, 0.44, 0.24 g L-1), MgSO4.7H2O (0.43, 0.37, 0.31 g L-1), KH2PO4 (0.13, 0.17, 0.21 g L-1), and the concentration of sucrose (25, 30, 35, 40 g L-1) were investigated in four independent experiments. The effects of the different studied factors were significant on the shoot regeneration. Results showed that pH 5.7 and the use of 35 g L-1 sucrose in MS medium were the best treatments for improving the number of shoots per explants (2.25 and 2 shoots, respectively). Moreover, increasing KH2PO4 concentration in MS medium produced the highest number of shoots per explant (3.5 shoots). The polypropylene container was also the best culture container for the lisianthus micropropagation (7.5 shoots per explant). | ||
| کلیدواژهها | ||
| Culture container؛ Lisianthus؛ Macro elements؛ Micropropagation؛ pH | ||
| مراجع | ||
|
Alanagh EN, Garoosi G, Haddad R, Maleki S, Landín M and Gallego PP, 2014. Design of tissue culture media for efficient Prunus rootstock micropropagation using artificial intelligence models. Plant Cell, Tissue and Organ Culture 117: 349-359.
Ballarin-Denti A and Antoniotti D, 1991. An experimental approach to pH measurement in the intercellular free space of higher plant tissues. Experientia 47 (5): 478-482.
Danci O and Danci M, 2008. The comparison between four potato cultivars multiple axillary bud micropropagation system efficiency. Scientific Papers Animal Science and Biotechnologies 41: 64-68.
De Klerk GJ, Hanecakova J and Jasik J, 2008.Effect of medium-pH and MES on adventitious root formation from stem disks of apple. Plant Cell, Tissue and Organ Culture 95 (3): 285–292
Demo P, Kuria P, Nyende A B and Kahangi EM, 2008. Table sugar as an alternative low cost medium component for in vitro micro-propagation of potato (Solanum tuberosum L.). African Journal of Biotechnology 7 (15): 2578-2584.
Edwin FG, Hall MA and De Klerk GJ, 2008. Plant Propagation by Tissue Culture. Spinger, Dordrecht, The Netherlands, 479 pp.
Furukawa H, Matsubara C and Shigematsu N, 1990. Shoot regeneration from the roots of prairie gentian (Eustoma grandiflorum (Griseb.) Schinners). Plant Tissue Culture Letters 7 (1): 11-13.
Gabryszewska E, 1996. The influence of temperature, day length and sucrose concentration on the growth and development of Alstroemeria. Acta Agrobotanica 49: 131-140.
Ghaffari Esizad S, Kaviani B, Tarang AR and Bohlooli Zanjani S, 2012. Micropropagation of lisianthus, an ornamental plant. Plant Omics Journal 5: 314-319.
George EF, Hall MA and De Klerk GJ (eds.), 2008. Plant propagationby tissue culture. Vol 1. The background, 3rd edition. Springer, Dordrecht.
Gerenda’s J and Sattelmacher B, 1999. Influence of nitrogen form and concentrations on growth and ionic balance of tomato and potato. Plant Nutrition Physiology and Applications 2: 33-37.
Hajnajari H, Hasanlou T, Hoshmand-Asghari A and Izadpanah M, 2008. Effects of different sources of nitrogen on in vitro growth characteristics of a selected genotype of wild cherry (Prunus savium L.). Seed and Plantlet 24: 749-762.
Harbage JF, Stimart DP and Auer C, 1998. pH affects 1h-indole-3-butyric acid uptake but not metabolism during the initiation phase of adventitious root induction in apple microcuttings. Journal of the American Society for Horticultural Science 123: 6-10.
Hazarika BN, ParthasarathyV A and Nagaraju V, 2004. Influence of in vitro preconditioning of Citrus Sp. micro shoots with sucrose on their ex vitro establishment. Indian Journal of Horticulture 61: 29-31. Hyndaman SE, Hasegawa PM and Bressan RA, 1982. The role of sucrose and nitrogen in adventitious root formation on cultured rose shoots. Plant Cell, Tissue and Organ Culture 1: 229– 238.
Islam Md T, Dembele DP and Keller ERJ, 2005. Influence of explant, temperature and different culture vessels on in vitro culture for germplasm maintenance of four mint accessions. Plant Cell, Tissue and Organ Culture 81: 123-130.
Jamal Uddin AFM, Rahaman Sk S, Ahmad H, Parvin S and Momena K, 2017. In vitro regeneration of lisianthus (Eustoma grandiflorum Grise). International Journal of Business, Social and Scientific Research 5: 126-135.
Kaviani B, Zamiraee F, Bohlooli Zanjani S, Tarang AR and Torkashvand AM, 2014. In vitro flowering and micropropagation of lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA). Acta Scientiarum Polonorum Hortorum Cultus 13 (4): 145-155.
Kornova K and Popov S, 2007. Effect of the in vitro container type on growth characteristics of the microplants in in vitro propagation of GF 677. Acta Horticulturae 825: 277–282.
Kozai T, Koyama Y and Watanabe I, 2002. Multiplication of potato plantlets in vitro with sugar-free medium under high photosynthetic photon flux. Acta Horticulturae 230: 121-128. Kunitake H, Nakashima T, Mori K, Tanaka M and Mii M, 1995. Plant regeneration from mesophyll protoplasts of lisianthus (Eustoma grandiflorum) by adding activated charcoal into protoplast culture medium. Plant Cell, Tissue and Organ Culture 43: 59-65.
Lakes C and Zimmerman RH, 1990. Impact of osmotic potential on in vitro culture of apple. Acta Horticulturae 280: 417-424.
Mousavi ES, Behbahani M, Hadavi E, Miri SM and Karimi N, 2012. Plant regeneration in Eustoma grandiflorum axillaries buds (Gentinaceae). Trakia Journal of Sciences 10 (2): 75-78.
Murashige T and Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
O’Brien IEW and Lindsay GC, 1993. Protoplasts to plants of Gentianaceae. Regeneration of lisianthus (Eustoma grandiflorum) is affected by calcium ion preconditioning, osmolality and pH of the culture media. Plant Cell, Tissue and Organ Culture 33: 31-37.
Paek KY and Hahn EJ, 2000. Cytokinins, auxins and active charcoal affects organogenesis and anatomical characteristics of shoot-tip cultures of lisianthus [Eustoma grandiflorum (Raf.) Shinn]. In Vitro Cellular & Developmental Biology - Plant 36: 128-132.Semeniuk P and Griesbach RJ, 1987. In vitro propagation of prairie gentian. Plant Cell, Tissue and Organ Culture 8: 249-253.
Shinohara N, Sugiyama M and Fukuda H, 2006. Higher extracellular pH suppresses tracheary element differentiation by affecting auxin uptake. Planta 224: 394-404.
Rahman MH and Alsadon AA, 2007. Photoautotrophic and photomixotrophic micropropagation of three potato cultivars. Journal of Biosciences 15: 111-116. Ramage CM and Williams RR, 2002. Mineral nutrition and plant morphogenesis. In Vitro Cellular & Developmental Biology-Plant 38 (2): 116-124.
Roh SM and Lawson RH, 1984. Tissue culture in the improvement of Eustoma. Horticultural Science 8: 23-658. | ||
|
آمار تعداد مشاهده مقاله: 674 تعداد دریافت فایل اصل مقاله: 742 |
||