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Callus Induction and Shoot Regeneration Using Indole Acetic Acid and N-Isopentenylamino Purine Combinations and Two Types of Explant in Tomato | ||
| Journal of Plant Physiology and Breeding | ||
| مقاله 5، دوره 5، شماره 2، اسفند 2015، صفحه 41-50 اصل مقاله (664.74 K) | ||
| نویسندگان | ||
| Tahere Fazeli Behgo1؛ Saadollah Alizadeh Ajirlo* 2 | ||
| 1Former MSc student of Horticulture, Department of Horticultural Science, University of Tabriz, Tabriz, Iran | ||
| 2Department of Landscape Architecture, University of Tabriz, Tabriz, Iran | ||
| چکیده | ||
| Effects of different concentrations of IAA (0, 0.3, 0.6 mg/l) and 2ip (0, 0.3, 0.6, 0.9, 1.2 mg/l) and their combinations on callus induction and shoot regeneration of hypocotyl and its thin cell layer (TCL) explants in tomato was studied. Explants were prepared from hypocotyls of seedlings in the aseptic condition. Hypocotyl segments were more efficient than TCL explants for callus induction and it was occurred on the 96.0 percent of hypocotyl explants as compared to 76.5 percent of TCL explants. The mean diameter of formed calli on the hypocotyl explants were significantly more than TCL explants. The calli on hypocotyl explants were more regenerative than calli produced on TCL explants and 60.1 percent of calli produced on hypocotyl explants developed the shoots while the regenerated shoots from the calli of TCL explants were 21.45 percent. Percentage of shoot induction on TCL explants was maximum (47.44 percent) in the medium containing 0.3 mg/l 2ip and 0.6 mg/l of IAA. Explant typehad significant influence on the shoot number per callus. Calli developed on TCL explants regenerated more shoots significantly than hypocotyl explants. The recorded shoot number per callus of TCL and hypocotyl explants were 6.45 and 3.22, respectively. The mean length of developed shoots on the hypocotyl segments was significantly higher than that of TCL explants and maximum length of shoot was obtained on the medium containing only 0.3 mg/l IAA. | ||
| کلیدواژهها | ||
| Callus؛ Hypocotyl؛ Shoot؛ TCL؛ Tomato | ||
| مراجع | ||
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Bansal M, Gaurva P, Sharmila P, Saradhi PP, Dilbaghi N and Chaudhury A, 2007. High frequency plant regeneration from cotyledonary explants of tomato (Lycopersicon esculentum Mill. cv. Pusa Ruby). Annals of Agri Bio Research 12 (2): 101-106.
Bhatia P and Ashwath N, 2008. Improving quality of in vitro cultured shoots of tomato (Lycopersicon esculentum Mill cv. Red Coat). Biotechnology 7(2): 188-193.
Biswas KK, Mohri T, Kogawara S, Hase Y, Narumi I and Oono Y, 2012. An improved system for shoot regeneration from stem explants of Lombardy poplar (Populus nigra L. var. Italica Koehne). American Journal of Plant Sciences 3: 1181-1186.
Brasileiro ACR, Willadino L, Carvalheira GG and Guerra M, 2006. Callus induction and plant regeneration of tomato (Lycopersicon esculentum cv. Ipa 5) via anther culture. Plant Cell, Tissue and Organ Culture 29 (4): 619-623.
Chaudhary Z, Abbas S, Yasin A, Rashid H, Ahmed H and Muhammad AA, 2010. Tissue culture studies in tomato (Lycopersicon esculentum var. Money Maker). Pakistan Journal of Botany 42 (1): 155-163.
Compton ME and Veilleus REE, 1990. Shoot, root and flower morphogenesis on tomato inflorescence explants. Plant Cell, Tissue and Organ Culture 24 (3): 223-231.
Davis PJ, 1999. Biosynthesis, Signal Transduction, Action. Kluwer Academic Publisher. 694 pp.
El-Awady AMM, El-Tarras AEA, El Dessoky SD, Essam El-Den MK and Ibrahim NE, 2014. Enhancement of salt tolerance of the tomato cultivar Edkawy under saline conditions using genetic transformation with the AtNHX1 gene. American Journal of Research Communication 2(4): 1-13.
Ghnaya BA, Charles G and Branchard M, 2008. Rapid shoot regeneration from thin cell layer explant excised from petioles in four cultivars of Brassica napus L. Plant Cell, Tissue and Organ Culture 92: 25-30.
Heba GA, Hussein GM and Abdallah NA, 2008. A rapid and efficient tomato and regeneration transformation system. Agriculture and Forestry Research 58: 103-111.
Jabeen N, Zubeda C, Rashid H and Mirza B, 2005. Effect of genotype and explant type on in vitro shoot regeneration of tomato (Lycopersicon esculentum Mill). Pakistan Journal of Botany 37 (4): 899-903.
Mamidala P and Nanna RS, 2009. Efficient in vitro plant regeneration, flowering and fruiting of dwarf tomato cv. Micro-Msk. Plant Omics Journal 2 (3): 98-102.
Mohnen D, Eberhard S, Marfa V, Doubrava N, Toubart P, Gollin DJ, Gruber TA, Nuri W, Albershiem P and Darvill A, 1990. The control of root, vegetative shoot and flower morphogenesis in tobacco thin cell layer explants (TCLs). Development 108 (1): 191-201.
Muthuvel M, Jawahar M, Rajendran A and Jayabalan N, 2005. An efficient protocol for organogenic callus induction and plant regeneration in tomato (Lycopersicon esculentum Mill). Plant Cell Biotechnology and Molecular Biology 6 (1&2): 41-46.
Nasib A, Ali K and Khan S, 2008. An optimized and improved method for the in vitro propagation of kiwifruit (Actinidia deliciosa) using coconut water. Pakistan Journal of Botany 40 (6): 2355-2360.
Nhut DT, Teixeira da Silva JA and Aswath CR, 2003. The importance of the explants on regeneration in thin cell layer technology. In Vitro Cellular & Developmental Biology - Plant 39 (3): 266–276.
Ohki S, Bigot C and Mousseau J, 1978. Analysis of shoot forming capacity in vitro in two lines of tomato (Lycopersicon esculentum Mill) and their hybrids. Plant and Cell Physiology 19: 27-42.
Osman MG, Elsadig A, Elhadi EA and Khalafala MM, 2010. Callus formation and organogenesis of tomato (Lycopersicon esculentum Mill cv. Omdurman). African Journal of Biotechnology 9 (28): 4407-4413.
Philip B, Sawhney RA and Galston AW, 1994. Isatin as an auxin source favoring floral and vegetative shoot regeneration from calli produced by thin layer explants of tomato pedicel. Plant Growth Regulation 15 (1): 17-21.
Rashid R and Bal SS, 2010. Effects of hormones on direct shoot regeneration in hypocotyl explants of tomato. Notulae Scientia Biologicae 2 (1): 70-73.
Reinhardt D, Mandel T and Kuhlemeier C, 2000. Auxin regulate the initiation and radial position of plant lateral organs. The Plant cell 12 (4): 507-518.
Sheeja TE and Mandal AB, 2003. In vitro flowering and fruiting in tomato (Lycopersicon esculentum Mill.). Asia Pacific Journal of Molecular Biology and Biotechnology 11 (1): 37-42.
Sink KC and Reynolds JF, 2004. Tomato (Lycopersicon esculentum Mill.). In Bajaj YPS (Ed.). Biotechnology in Agriculture and Forestry, 12: Crops I. Pp. 319-344. Springer Verlag Berlin.
Tran Thanh Van M, 1980. Control of morphogenesis by inherent and exogenously applied factors in thin cell layers. International Review of Cytology (Supplement) 11A: 175–194.
Tiexeira da Silva JA, 2003. Thin cell layer technology in ornamental plant micropropagation and biotechnology. African Journal of Biotechnology 2 (12): 683-691.
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