آمار
| تعداد نشریات | 44 |
| تعداد شمارهها | 1,348 |
| تعداد مقالات | 16,508 |
| تعداد مشاهده مقاله | 53,557,435 |
| تعداد دریافت فایل اصل مقاله | 16,046,068 |
Alleviation of Adverse Effects of Copper on Allium cepa L. by Exogenous Ascorbic Acid Application | ||
| Journal of Plant Physiology and Breeding | ||
| مقاله 1، دوره 3، شماره 2، اسفند 2013، صفحه 1-12 اصل مقاله (144.24 K) | ||
| نوع مقاله: Research Paper | ||
| چکیده | ||
| Abstract In this study ameliorative effects of ascorbic acid (0.5 mM) on onion (Allium cepa L. cv. Red Azarshahr) under copper stress (Cu: 6.5 μM) were studied. Fresh weights of leaves and roots were reduced in seven week old plants under excess copper condition. Cu stress also reduced membrane stability index in leaves. There was a considerable increase in H2O2 content and lipid peroxidation in both roots and leaves of plants under excess Cu. Leaves anthocyanin contents, leaves and roots phenolic compounds, soluble proteins and proline contents were increased in response to Cu toxicity. The fresh weights of leaves and roots were improved during addition of ascorbic acid to root medium in stressed and non-stressed plants. Exogenous ascorbic acid significantly increased membrane stability index in Cu stressed plants. We find considerable reduction in anthocyanins, phenolic compounds and proline with ascorbic acid treatments in all plants. Ascorbic acid application also reduced H2O2 content and prevented lipid peroxidation in leaves and roots during presence or absence of excess Cu. Treatment of plants with ascorbic acid increased soluble proteins content only in non-stressed plants. The results demonstrate that root applied ascorbic acid ameliorated the copper induced oxidative adverse effects on onion growth. | ||
| کلیدواژهها | ||
| Keywords: Allium cepa L؛ Antioxidant؛ Ascorbic acid؛ Copper؛ Membrane | ||
| مراجع | ||
|
Alqurainy F, 2007. Responses of bean and pea to vitamin C under salinity stress. Agriculture and Biological Sciences 3: 714-722.
Athar HR, Khan A and Ashraf M, 2008. Exogenously applied ascorbic acid alleviates salt-induced oxidative stress in wheat. Environmental and Experimental Botany 63: 224–231.
Avery SV, 2001. Metal toxicity in yeasts and the role of oxidative stress. Advanced Application Microbiology 49: 111-142.
Azooz M and Al-Fredan M, 2009. The inductive role of vitamin C and its mode of application on growth, water status, antioxidant enzyme activity and protein pattern of Vicia faba L. cv. Hassami growth under seawater irrigation. American Journal of Plant Physiology 4: 38-51.
Azzedine F, Cherroucha H and Baka M, 2011. Improvement of salt tolerance in durum wheat by ascorbic acid application. Journal of Stress Physiology and Biochemistry 7: 27-37.
Bassuony F, Hassanein R, Baraka D and Kalil R, 2008. Physiological effects of nicotinamide and ascorbic acid on Zea mays plant grown under salinity stress. II. Changes in nitrogen constituent, protein profiles, protease enzyme and certain inorganic cations. Australian Journal of Basic and Applied Sciences 2: 350-359.
Bates LS, Waldren RP and Teare ID, 1973. Rapid determination of proline for water stress studies. Plant and Soil 39: 205–207.
Bradford M, 1976. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Annual Biochemistry 72: 248-254.
Chen CT, Chen LM, Lin CC and Kao CH, 2001. Regulation of proline accumulation in detached rice leaves exposed to excess copper. Plant Science 160: 283-290.
Cobbett C and Goldbrough P, 2002. Phytochelatins and metallothioneins: roles in leaves metal detoxification and homeostasis. Annual Review Plant Biology 53: 159-182.
Colville L and Smirnoff N, 2008. Antioxidant status, peroxidase activity, and PR protein transcript levels in ascorbate-deficient Arabidopsis thaliana vtc mutants. Journal of Experimental Botany 59: 3857-3868.
Cui XM, Zhang YK, Wu XB and Liu CS,2010. The investigation of the alleviated effect of copper toxicity by exogenous nitric oxide in tomato plants. Plant, Soil and Environment 56: 274–281.
Cuypers A, Vangronsveld J and Clijsters H,2000. Biphasic effect of copper on the ascorbate-glutathione pathway in primary leaves of Phaseolus vulgaris seedlings during the early stages of metal assimilation. Physiologia Plantarum 110: 512–517.
Davey MW, Managu MV, Dirk I, Maite S, Angelos K, Smirnoff N, Binenzir IJJ, Strain JJ, Favell D and Fletcher J, 2000. Plant ascorbic acid: chemistry, function, metabolism, bioavailbility and effects of processing. Sciences of Food and Agriculture 80: 825-860.
Demirevska-Kepova K, Simova-Stoilova L, Stoyanova Z, Holzer R and Feller U, 2004. Biochemical changes in barely plants after excessive supply of copper and manganese. Environmental and Experimental Botany 52: 253–266.
Dolatabadian A, Sanavy SAMM and Chashmi NA, 2008. The effects of foliar application of ascorbic acid (vitamin C) on antioxidant enzymes activities, lipid peroxidation and proline accumulation of canola (Brassica napus L.) under conditions of salt stress. Journal of Agronomy and Crop Science 194: 206-213.
Dolatabadian A, Sanavy SAMM and Sharifi M, 2009. Alleviation of water deficit stress effects by foliar application of ascorbic acid on Zea mays L. Journal of Agronomy and Crop Science 195: 347-355.
Eid RA, Taha LS and Ibrahim SMM, 2011. Alleviation of adverse effects of salinity on growth, and chemical constituents of marigold plants by using glutathione and ascorbate. Journal of Applied Sciences Research 7: 714-721.
El-Lethy SR, Ayad HS and Reda F, 2011. Effect of riboflavin, ascorbic acid and dry yeast on vegetative growth, essential oil pattern and antioxidant activity of geranium (Pelargonium graveolens L.). American- Eurasian Journal of Agricultural and Environmental Sciences 10: 781-786.
El-Tayeb M and El-Enany A, 2006. Salicylic acid induced adaptive response to copper stress in sunflower (Helianthus annuus L.). Plant Growth Regulation 50: 191-199.
Foyer CH and Noctor G, 2011. Ascorbate and glutathione: the heart of redox hub. Plant Physiology 155: 2-18.
Gorecka K, Cvikrova M, Kowalska U, Eder J, Ska K, Gorecki R and Janas KM, 2007. The impact of Cu treatment on phenolic and polyamine levels in plant material regenerated from embryos obtained in anther culture of carrot. Plant Physiology and Biochemistry 45: 54-61.
GouldKS, 2004. Nature’s swiss army knife: the diverse protective roles of anthocyanins in leaves. Journal of Biomedicine and Biotechnology 5: 314–320.
Groppa MD, Ianuzzo MP, Tamaro ML and Benavides MP, 2007. Polyamine metabolism in sunflower plants under long term cadmium or copper stress. Amino Acids 32: 265-275.
Hall JL, 2002. Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany 53: 1-11.
Hansch R and Mendel RR, 2009. Physiological functions of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl). Current Opinion in Plant Biology 12: 259 - 266.
Hariri DME, Sadak MS and El-Bassiouny HMS, 2010. Response of flax cultivars to ascorbic acid and α-tocopherol under salinity stress conditions. Academic Research 2: 101-109.
Heat RL and Packer L, 1968. Photoperoxidation in isolated chloroplasts. I: kinetics and stoichiometry of fatty acid peroxidation. Archive of Biochemistry and Biophysics 125: 189-198.
Jana S and Chaudhuri MA, 1981. Glycolate metabolism of three submerged aquatic angiosperms during aging. Aquatic Botany 12: 345-54.
Khan TA, Mazid M and Mohammad F, 2011. A review of ascorbic acid potentialities against oxidative stress induced in plants. Journal of Agrobiology 28: 97–111.
Kumar S, Kaur R, Kaur N, Bhandhari K, Kaushal N, Gupta K, Bains TS and Nayyar H, 2011. Heat stress induced inhibition in growth and chlorosis in mung bean (Phaseolus aureus Roxb.) is partly mitigated by ascorbic acid application and is related to reduction in oxidative stress. Acta Physiologiae Plantarum 33: 2091- 2101.
Lombardi L and Sebastiani L, 2005. Copper toxicity in Prunus cerasifera: growth and antioxidant enzymes responses of in vitro grown plants. Plant Science 168: 797-802.
Michalak A, 2006. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress.Polish Journal of Environmental Study 15: 523-530.
Mourato MP, Martins LL and Campos-Andrada MP, 2009. Physiological responses of Lupinus luteus to different copper concentrations. Biologia Plantarum 53: 105-111.
Panda SK, 2008. Impact of copper on reactive oxygen species, lipid peroxidation and antioxidants in Lemna minor. Biologia Plantarum 52: 561-564.
Sairam RK, Singh DV and Srivastava GC,2003. Changes in activities of antioxidant enzymes in sunflower leaves of different ages. Biologia Plantarum 47: 61-66.
Salama KH, 2009. Amelioration of NaCl-induced alterations on the plasma membrane of Allium Cepa L.by ascorbic acid. Australian Journal of Basic and Applied Sciences 3: 990-994.
Sanchez-Viveros G, Gonzalez-Mendoza D, Alarcon A and Ferrera-Cerrato R, 2010. Copper effects on photosynthetic activity and membrane leakage of Azolla filiculoides and A. caroliniana. International Journal of Agriculture and Biology 12: 365–368.
Schutzenduble A and Polle A, 2002. Plant responses to abiotic stresses: heavy metal induced oxidative stress and protection by mycorrhization. Journal of Experimental Botany 53: 1352-136.
Sgherri C, Cosi E and Navari-Izzo F, 2003. Phenols and antioxidative status of Raphanus sativus grown in copper excess. Physiologia Plantarum 118: 21-28.
Shalata A and Neumann PM, 2001. Exogenous ascorbic acid (vitamin c) increases resistance to salt stress and reduces lipid peroxidation. Journal of Experimental Botany 52: 2207-2211.
Sharma SS and Dietz KJ, 2006. The significance of amino acids and amino acid derived molecules in plant responses and adaptation to heavy metal stress. Journal of Experimental Botany 57: 711–726.
Shin LJ, Lo JC and Yeh KC, 2012. Copper chaperone antioxidant Protein1 is essential for copper homeostasis. Plant Physiology 159: 1099–1110.
Singleton VL and Rossi JA, 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16: 144-158.
Smirnoff N and Wheeler GL, 2000.Ascorbic acid in plants: biosynthesis and function.Critical Reviews inBiochemistry and Molecular Biology35: 291-314.
Suresh B and Ravishankar G, 2004. Phytoremediation a novel and promising approach for environmental clean-up. Critical Reviews in Biotechnology 24: 97-124.
Tamas L, Budikova S, Simonovicova M, Huttova J, Siroka B and Mistrik I, 2006. Rapid and simple method for Al toxicity analysis in emerging barley roots during germination. Biologia Plantarum 50: 87–93.
Thapa G, Sadhukhan A, Panda SK and Sahoo L, 2012. Molecular mechanistic model of plant heavy metal tolerance. Biometals 25: 489-505.
Wanger GJ, 1979. Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplast.Plant Physiology 64: 88-93.
Xiong ZT, Liu C and Geng B, 2006. Phytotoxic effects of copper on nitrogen metabolism and plant growrh in Brassica pekinsis Rupr. Ecotoxicology and Environmental Safety 64: 273-280.
Yuan HM, Xu HH, Liu WC and Lu YT, 2013. Copper regulates primary root elongation through PIN1-mediated auxin redistribution. Plant Cell Physiology 54: 766-778.
Younis ME, Hasaneen MNA and Kazamel AMS, 2010. Exogenously applied ascorbic acid ameliorates detrimental effects of NaCl and mannitol stress in Vicia faba seedlings. Protoplasma 239: 39-48.
Yruela I, 2009. Copper in plants: acquisition, transport and interactions. Functional Plant Biology 36: 409-430.
Yurekli F and Porgali ZB, 2006. The effects of excessive exposure to copper in bean plants. Acta Biologica Cracovirnsia Series Botanica 48: 7-13. | ||
|
آمار تعداد مشاهده مقاله: 2,522 تعداد دریافت فایل اصل مقاله: 2,834 |
||