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Effect of various phenol inhibitors and explant size on meristem culture in the Gavieta variety of Fragaria ananassa | ||
| Journal of Plant Physiology and Breeding | ||
| دوره 13، شماره 2، 2023، صفحه 1-13 اصل مقاله (752.24 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2023.17090 | ||
| نویسندگان | ||
| Sanaz Hemmati asl؛ Ebrahim Dorani* | ||
| Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran | ||
| چکیده | ||
| Strawberry (Fragaria ananassa cv. Gavieta) are infected with several important viral diseases. In vitro culture of the meristem-tip is a valuable method in recovering pathogen-free plants and micropropagation of such crops. In this study, the effects of meristematic dome size, medium type and phenol inhibitors (AC, PVP and ascorbic acid) were investigated on the shoot tip culture of strawberry. Also the effect of plant growth regulators on shoot elongation was investigated. As the base medium, shoot tips were cultured on the MS media supplemented with 1 mg/l 6-benzylaminopurine (BAP) and 0.2 mg/l indole-3-acetic acid (IAA). The highest regenerated shoots were obtained with the meristematic dome with two leaves. The type of medium (liquid, semi-solid, and solid) significantly affected shoot tip proliferation, and the highest percentage of shoots were observed in the semi-solid medium. In the semi-solid medium all meristems were regenerated and produced more shoots with 8.55 mm length. Application of the phenol-absorbing and antioxidant compounds in the culture medium improved shoot induction and shoot performance. The best result was obtained in the medium supplemented with 500 mg/l polyvinylpyrrolidone (PVP) with 5 shoots, in which browning and explant death were avoided by absorbing phenolic substances from the explant. | ||
| کلیدواژهها | ||
| micropropagation؛ phenol-absorbing and antioxidant compounds؛ stem؛ strawberry | ||
| مراجع | ||
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Banaeian N, Omid M, Ahmadi H, 2011. Energy and economic analysis of greenhouse strawberry production in Tehran province of Iran. Energy Conversion and Management 52:1020-1025.
Debnath S, Teixeira da Silva J, 2007. Strawberry culture in vitro: applications in genetic transformation and biotechnology. Fruit, Vegetable and Cereal Science and Biotechnology 1:1-12
FAO.http://www.fao.org/state-of foodagriculture/2019/en/https://www.fao.org/biotech/conf12.htm
Hollings M (1965) Disease control through virus-free stock. Annual Review of Phytopathology 3:367-396. https://doi.org/10.1146/annurev.py.03.090165.002055
Jadwiga I, Czernas J, Gawronski J, Hortynski J, 2003. Suitability of strawberry (Fragaria x ananassa Duch.) microplants to the field cultivation. J Food Agric Environ 1:190-193
Kartha K, 1985. Meristem culture and germplasm preservation. Cryopreservation of Plant Cells and Organs, pp 115-134
Ko J, Kim Y, Lee J, Kim H, 2000. Micropropagation from corm apical meristems culture of Freesia refrecta hybrida. Horticultural Science and Technology 18:733-733
Ko W, Su C, Chen C, Chao C, 2009. Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid. Plant Cell, Tissue and Organ Culture (PCTOC) 96(2):137-141
Li W, Masilamany P, Kasha K, Pauls K, 2002. Developmental, tissue culture, and genotypic factors affecting plant regeneration from shoot apical meristems of germinated Zea mays L. seedlings. In Vitro Cellular and Developmental Biology-Plant 38: 285-292
Lizarraga R, Salazar L, 1983. Effect of meristem size on eradication of potato spindle tuber viroid (Solanum tuberosum). In International Congress Research for the Potato, pp 22-27
Martin R, Tzanetakis I, 2006. Characterization and recent advances in detection of strawberry viruses. Plant Disease 90:384-396
Mehrotra S, Goel M, Kukreja A, Mishra B, 2007. Efficiency of liquid culture systems over conventional micropropagation: A progress towards commercialization. African Journal of Biotechnology 6:25-32
Nemeth M, 1984. Virus, mycoplasma, and rickettsia diseases of fruit trees. Springer Book Archive, pp 456-480
Ngomuo M, Mneney E, Ndakidemi P, 2014. The in vitro propagation techniques for producing banana using shoot tip cultures. American Journal of Plant Sciences 5:56-62. DOI:10.4236/ajps.2014.511175
Quiroz K, Berrios M, Carrasco B, Retamales J, Caligari P, García-Gonzáles R, 2017. Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis L. Duch.). Biological research 50:214-220. http://dx.doi.org/10.1186/s40659-017-0125-8
Rattanpal H, Gill M, and Sangwan A, 2011. Micropropagation of strawberry through meristem culture. Acta Horticulturae 890:149-153. 10.17660/ActaHortic.2011.890.19
Sinclair W, Lyon H, 2005. Diseases of trees and shrubs. Comstock Publishing Associates, pp 225-240.
Thompson J, Jelkmann W, 2003. The detection and variation of Strawberry mottle virus. Plant Disease 87:385-390. https://doi.org/10.1094/PDIS.2003.87.4.385
Titov S, Bhowmik S, Mandal A, Alam M, Uddin S, 2006. Control of phenolic compound secretion and effect of growth regulators for organ formation from Musa spp. cv. Kanthali floral bud explants. Am. J. Biochem. Biotechnol 2:97-104
Torregrosa M, Genesca J, Gonzalez A, Evangelista A, Mora A, Margarit C, Guardia J, 2001. Role of Doppler echocardiography in the assessment of portopulmonary hypertension in liver transplantation candidates1. Transplantation 71:572-574
Verma N, Ram R, Hallan V, Kumar K, Zaidi A, 2004. Production of Cucumber mosaic virus-free chrysanthemums by meristem tip culture. Crop Protection 23:469-473. https://doi.org/10.1016/j.cropro.2003.08.021
Vine S, 1968. The culture of gooseberry shoot tips for eliminating virus. Journal of Horticultural Science 43:289-292
Wang P, Hu C, 1980. Regeneration of virusfree plants through in vitro culture. In Advances in Biomedical Engineering 18:61-99. DOI: 10.1007/3-540-09936-0_3
Wang Q, Mawassi M, Li P, Gafny R, Sela I, Tanne E, 2003. Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L. Plant Science 165:321-327. https://doi.org/10.1016/S0168-9452(03)00091-8 | ||
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