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فهرست نشریات دارای اعتبار وزارت علوم، تحقیقات و فناوری
پایگاه استنادی علوم جهان اسلام (ISC)

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Mehdizadeh, Mostafa, Mohsenzadeh Golfazani, Mohammad, Samizadeh Lahiji, Habibollah. (1402). Assessment of genetic diversity in different types of tobacco using molecular markers. سامانه مدیریت نشریات علمی, (), 85-105. doi: 10.22034/jppb.2023.16404
Mostafa Mehdizadeh; Mohammad Mohsenzadeh Golfazani; Habibollah Samizadeh Lahiji. "Assessment of genetic diversity in different types of tobacco using molecular markers". سامانه مدیریت نشریات علمی, , , 1402, 85-105. doi: 10.22034/jppb.2023.16404
Mehdizadeh, Mostafa, Mohsenzadeh Golfazani, Mohammad, Samizadeh Lahiji, Habibollah. (1402). 'Assessment of genetic diversity in different types of tobacco using molecular markers', سامانه مدیریت نشریات علمی, (), pp. 85-105. doi: 10.22034/jppb.2023.16404
Mehdizadeh, Mostafa, Mohsenzadeh Golfazani, Mohammad, Samizadeh Lahiji, Habibollah. Assessment of genetic diversity in different types of tobacco using molecular markers. سامانه مدیریت نشریات علمی, 1402; (): 85-105. doi: 10.22034/jppb.2023.16404

Assessment of genetic diversity in different types of tobacco using molecular markers

Journal of Plant Physiology and Breeding
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 05 تیر 1402
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22034/jppb.2023.16404
نویسندگان
Mostafa Mehdizadeh1؛ Mohammad Mohsenzadeh Golfazani* 2؛ Habibollah Samizadeh Lahiji3
1Researcher, Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2Assistant Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.
چکیده
I
Information about the amount of genetic diversity in the germplasm and genetic relations of genotypes is essential for analyzing and designing breeding programs and could be used for assisting in genetic identification and improvement of the germplasm. In this research, the genetic diversity of 48 genotypes of the flue-cured burley and oriental type of tobacco, was investigated by 12 ISSR, 10 SSR, and five IRAP primers. The maximum number of bands was observed in the UBC825, RTR-10, and mixed primer UBC817+UBC826 with 17 bands, UBC817 with 16 bands, and RTR-8 with 15 bands. UBC824 with 10 and UBC823 with 11 bands showed the minimum numbers of bands among ISSR and IRAP primers, and PT30044 and PT30046 primers with two alleles showed the minimum number of alleles among SSR markers. In ISSR and IRAP, the observed percentage of polymorphism ranged from 76.92% for RTR-1 and RTR-7 to 94.11% for RTR-10 and in SSR markers, it was 100%. The average polymorphism percentage was 90.7%, which indicated enough genetic diversity among the tobacco genotypes. The polymorphism information content (PIC) ranged from 0.31 to 0.5 with an average of 0.42. The average diversity of Nei and Shannon indices were 0.39 and 0.58, respectively. Cluster analysis by the UPGMA method classified the 48 genotypes into five groups, containing 5, 12, 10, 6, and 15 genotypes. Canonical discriminate analysis via Fisher’s linear model confirmed the validity of the cluster analysis result (85.4 percent). Principal coordinate analysis showed that the 14 first components could explain 51.49% of the total variance. The primers used in this study had high efficiency which can be used to study the genetic diversity in tobacco.
کلیدواژه‌ها
burley؛ flue-cured؛ IRAP؛ oriental؛ SSR
آمار
تعداد مشاهده مقاله: 111


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