آمار
| تعداد نشریات | 43 |
| تعداد شماره‌ها | 1,224 |
| تعداد مقالات | 15,038 |
| تعداد مشاهده مقاله | 50,578,901 |
| تعداد دریافت فایل اصل مقاله | 13,669,016 |
Design and fabrication of a microfluidic chip for oocyte denudation. | ||
| مجله مهندسی برق دانشگاه تبریز | ||
| مقاله 5، دوره 52، شماره 3 - شماره پیاپی 101، مهر 1401، صفحه 189-194 اصل مقاله (807.79 K) | ||
| نوع مقاله: علمی-پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/tjee.2022.15541 | ||
| نویسندگان | ||
| فیروز صفائی فر1؛ جواد کرمدل2؛ هادی ولادی* 3، 1؛ محمد فلاح تفتی4 | ||
| 1Biomedical Engineering Department, Faculty of Technical and Engineering South Tehran Branch, Islamic Azad University, Tehran, Iran. | ||
| 2Electrical Engineering Department, Faculty of Technical and Engineering South Tehran Branch, Islamic Azad University, Tehran, Iran | ||
| 3Microsystem Fabrication Laboratory, Faculty of Electrical and Computer Engineering, University of Tabriz, Tabriz, Iran. | ||
| 4Department of biology, Islamic Azad University, Tabriz Branch, Tabriz, Iran. | ||
| چکیده | ||
| Intracytoplasmic sperm injection (ICSI) is one of the most successful techniques of Assisted Reproductive Technology (ART) and is mostly in use for the treatment of infertility with male factors. In this method, before injecting sperm into the intracytoplasmic of the oocyte, cumulus cells around the oocyte must be stripped to facilitate the injection process. To achieve this, both enzymatic and mechanical methods are used in embryological laboratories for denudation, which has major deficiencies, including the possibility of damaging the oocyte prior to the injection process. In this research, a microfluidic-based device is introduced for the separation of cumulus cells around the oocyte with minimum manual operations. The results prove high efficiency, and non-destructive denudation of the oocyte with the reduced amount of culture medium leads to the low-cost preparation process of oocytes. The process can also be integrated with ICSI chips under development and will be reported shortly. | ||
| کلیدواژه‌ها | ||
| Assisted Reproductive Technology (ART)؛ Denudation؛ In Vitro Maturation (IVM)؛ Microfluidics؛ lab-on-chip | ||
| مراجع | ||
|
[1] Ombelet, Willem et al. “Infertility and the provision of infertility medical services in developing countries.” Human reproduction update, vol. 14, no. 6 , pp. 605-621, 2008.
[2] Nosrati, Reza et al. “Microfluidics for sperm analysis and selection.” Nature reviews Urology, vol. 14, no. 12 pp. 707-730, 2017.
[3] Kashaninejad, Navid, Muhammad Johirul Alam Shiddiky, and Nam‐Trung Nguyen. "Advances in microfluidics‐based assisted reproductive technology: From sperm sorter to reproductive system‐on‐a‐chip." Advanced Biosystems, vol. 2, n . 3, p. 1700197, 2018.
[4] Katz, Patricia, Robert Nachtigall, and Jonathan Showstack. "The economic impact of the assisted reproductive technologies." Nature Medicine, vol. 8, n. 10, pp. S29-S32, 2002.
[5] Smith, Gary D, and Shuichi Takayama. “Application of microfluidic technologies to human assisted reproduction.” Molecular human reproduction vol. 23, n. 4, pp. 257-268, 2017.
[6] Asghar W, Velasco V, Kingsley JL, et al. Selection of functional human sperm with higher DNA integrity and fewer reactive oxygen species. Adv Healthc Mater, vol. 3, no. 10, pp.1671-1679, 2014.
[7] Yan, Yimo et al. “A PMMA-Based Microfluidic Device for Human Sperm Evaluation and Screening on Swimming Capability and Swimming Persistence.” Micromachines vol. 11, no. 9, pp. 793, 21 Aug. 2020.
[8] Xie, Lan et al. “Integration of sperm motility and chemotaxis screening with a microchannel-based device.” Clinical chemistry vol. 56, no. 8, pp. 1270-1278, 2010.
[9] Liu, Xinyu et al. “In situ mechanical characterization of mouse oocytes using a cell holding device.” Lab on a chip, vol. 10, no. 16, 2154-2161, 2010.
[10] Han, Chao et al. “Integration of single oocyte trapping, in vitro fertilization and embryo culture in a microwell-structured microfluidic device.” Lab on a chip vol. 10, no. 21, pp. 2848-2854, 2010.
[11] Zeringue, H C et al. “Early mammalian embryo development depends on cumulus removal technique.” Lab on a chip vol. 5, no. 1, pp. 86-90, 2005.
[12] Suh, Ronald S et al. “IVF within microfluidic channels requires lower total numbers and lower concentrations of sperm.” Human reproduction (Oxford, England) vol. 21, no. 2, pp. 477-83, 2006.
[13] Ma, Rui et al. “In vitro fertilization on a single-oocyte positioning system integrated with motile sperm selection and early embryo development.” Analytical chemistry vol. 83, no. 8, pp. 2964-70, 2011.
[14]زرگری, سیاوش, ولادی, هادی, صادق زاده اسکوئی, بهناز, شهابی, پرویز, فرونچی, جواد, پاشائی اصل, مریم. (1395). طراحی و ساخت ریزتراشه بلوغ آزمایشگاهی تخمک. مجله مهندسی برق دانشگاه تبریز .46(3), 211-221. [15] Palermo, G et al. “Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte.” Lancet (London, England) vol. 340, no. 8810, pp. 17-8 , 1992.
[16] Boulet, Sheree L et al. “Trends in use of and reproductive outcomes associated with intracytoplasmic sperm injection.” JAMA vol. 313, no.3, pp. 255-63, 2015
[17] Rienzi, Laura et al. “The oocyte.” Human reproduction, vol. 27, no. Suppl, pp. i2-i21, 2012.
[18] Zeringue, Henry Chris et al. “Removal of Cumulus from Mammalian Zygotes using Microfluidic Techniques.” Biomedical Microdevices 3, no. 3, pp. 219-224,2001.
[19] Zeringue, H C et al. “Early mammalian embryo development depends on cumulus removal technique.” Lab on a chip vol. 5, no. 1, pp. 86-90, 2005
[20] Pelesko JA, Bernstein DH. Modeling mems and nems. Boca Raton,Florida: CRC press; 2002.
[21] Gaver, D. P., 3rd, & Kute, S. M, A theoretical model study of the influence of fluid stresses on a cell adhering to a microchannel wall. Biophysical journal,vol. 75, no. 2, pp. 721–733, 1998.
[22] Xie Y, Wang F, Zhong W, Puscheck E, Shen H, Rappolee DA. Shear stress induces preimplantation embryo death that is delayed by the zona pellucida and associated with stress-activated protein kinase-mediated apoptosis. Biol Reprod, vol. 75, no.1, pp. 45-55, 2006.
[23] Zeringue, H.; Rutledge, J.; Beebe, D. Early mammalian embryo development depends on cumulus removal technique. Lab Chip. vol. 5, no.1, pp. 86–90, 2005. | ||
|
آمار تعداد مشاهده مقاله: 304 تعداد دریافت فایل اصل مقاله: 236 |
||