آمار
| تعداد نشریات | 41 |
| تعداد شمارهها | 1,116 |
| تعداد مقالات | 13,677 |
| تعداد مشاهده مقاله | 48,550,479 |
| تعداد دریافت فایل اصل مقاله | 12,300,950 |
Assessment of genetic diversity in different types of tobacco using molecular markers | ||
| Journal of Plant Physiology and Breeding | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 09 آذر 1401 | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2022.51428.1270 | ||
| نویسندگان | ||
Mostafa Mehdizadeh1؛ Mohammad Mohsenzadeh Golfazani   2؛ Habibollah Samizadeh Lahiji3
| ||
| 1Plant Breeding Researcher, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 2Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 3Department of Agricultural Biotechnology, University of Guilan, Rasht, Iran | ||
| چکیده | ||
| Information about the amount of genetic diversity in germplasm and genetic relations of genotypes are essential for analyzing and designing breeding programs and could be used for assisting in genetic identification and improvement of the germplasm. Therefore, to manage a germpelasm source effectively, it is essential to reveal the extent and the structure of genetic diversity. According to the importance of identification and classification of germplasm, the genetic diversity of 48 genotypes of flue-cured, burley, and oriental type of tobacco, were investigated by 12 ISSR, 10 SSR, and five IRAP primers. The maximum number of bands was observed in UBC825, RTR-10 primers, and mixed primer UBC817+UBC826 with 17 bands, UBC817 primer with 16 bands and RTR-8 with 15 bands. UBC824 with 10 and UBC823 with 11 bands showed minimum numbers of bands among the ISSR and IRAP primers, and PT30044 and PT30046 primers with two alleles showed minimum number of alleles among SSR markers. In ISSR and IRAP, the observed percentage of polymorphism ranged from 76.92% for RTR-1 and RTR-7 to 94.11% for RTR-10 and in SSR markers, it was 100%. The average polymorphism percentage was 90.7%, which indicated the existance of adequate genetic diversity among genotypes. The polymorphism information content ranged from 0.31 to 0.5 with an average of 0.42. Average diversity obtained by Nei and Shannon indices were 0.39 and 0.58, respectively. Cluster analysis by the UPGMA method classified 48 genotypes into five groups, containing 5, 12, 10, 6, and 15 genotypes. Principal coordinate analysis showed that the first 14 components could explain 51.49% of the total variance. | ||
| کلیدواژهها | ||
| burley؛ flue-cured؛ IRAP؛ Oriental؛ SSR | ||
|
آمار تعداد مشاهده مقاله: 76 |
||
