آمار
| تعداد نشریات | 43 |
| تعداد شمارهها | 1,253 |
| تعداد مقالات | 15,411 |
| تعداد مشاهده مقاله | 51,245,948 |
| تعداد دریافت فایل اصل مقاله | 14,254,199 |
Effect of chitosan on regeneration and secondary metabolite production of Lilium regale | ||
| Journal of Plant Physiology and Breeding | ||
| دوره 11، شماره 2، اسفند 2021، صفحه 147-160 اصل مقاله (686.34 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2021.14581 | ||
| نویسندگان | ||
| Younes Pourbeyrami Hir* ؛ Mahsa Khalafi؛ Esmaeil Chamani؛ Hassan Maleki Lajayer | ||
| University of Mohaghegh Ardabili, Ardabil, Iran | ||
| چکیده | ||
| Lilium spp. belong to the Liliaceae family. This plant contains a valuable secondary metabolite which can be increased by the stimuli treatment. The main goal of the present study was to evaluate the effects of chitosan as a stimulant on regeneration and production of secondary metabolites of Lilium regale. The experiment was carried out as a completely randomized design in the MS medium with 10 replications. Five different concentrations (0, 50, 100, 150, and 200 mg/L) of chitosan were used as treatments. Different morphological traits, leaf chlorophyll, and secondary metabolites were measured. Analysis of variance showed that chitosan significantly affected plant height, fresh weight, bulblet number, root number, chlorophyll a and b, total chlorophyll, phenols, flavonoids, and regeneration percentage. The highest and lowest fresh weight and root number were obtained from 200 ppm of chitosan and the control, respectively. The results also showed that the plant height and bulblet number in all concentrations of chitosan were higher than the control. The highest and lowest regeneration rate were obtained from the 200 ppm chitosan and the control, respectively. However, the highest amount of phenols, flavonoids, and chlorophyll was obtained from the 200 ppm chitosan. According to the results of the present study, chitosan at the rate of 200 ppm had a more positive effect on regeneration and production of secondary metabolites of Lilium regale compared to other concentrations. | ||
| کلیدواژهها | ||
| Chitosan؛ In vitro culture؛ Lily flowers؛ Regeneration؛ Secondary metabolites | ||
| مراجع | ||
|
Ait-Barka E, Eullaffroy P, Clement C and Vernet G, 2004. Chitosan improves development, and protects Vitis vinifera L. against Botrytis cinerea. Plant Cell Report 22: 608-614.
Anonymous S, 2009. Lilies the Flower Expert-Flower Encyclopedia. http://www.theflowerexpert.com/content/mostpopularflowers/lilies
Arikat NA, Jawad FM, Karam NS and Shibli RA, 2004. Micropropagation and accumulation of essential oils in wild sage (Salvia fruticosa Mill). Scientia Horticulturea 100: 193-202.
Azadi P and Khosh-Khui M, 2007. Micropropagation of Lilium ledebourii bioss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments. Electronic Journal of Biotechnolgy10(4): 583-591.
Baker LG, Specht CA, Donlin MJ and Lodge JK, 2007. Chitosan the Deacetylati Form of chitin is Necessary for cell wall Integrity in Cryptococcus neoformans. Eukaryotic Cell 6 (5): 855-867.
Buschman JCM, 2004. Production of bulbs and bulb cut flowers in the world present and future. Ixth International symposium on flower bulbs. Niigata. Japan, 1-2.
Chinese Pharmacopoeia Commission 2020. Pharmacopoeia of the People’s Republic of China (Part I) 2020th ed. China Pharmaceutical Science and Technology Press; Beijing, China p. 137.
Cho MH, No HK and Prinyawiwatkul W, 2008. Chitosan treatments affect growth and selected quality of sunflower sprouts. Journal of Food Science 73: 570-577
Chu IYE and Kurtz SL, 1990. Commercialization of plant micropropagation. In: Ammirato PV, Evans DA, Sharp WR and Bajaj YPS (eds.). Handbook of Plant Cell Culture. Volume 5. Ornamental Species, pp. 126-164. McGraw-Hill Publishing Company, New York, USA.
Coqueiro DSO, Maraschin M and Piero RMD, 2011. Chitosan Reduces Bacterial Spot Severity and Acts in Phenylpropanoid metabolism in Tomato Plants. Journal of Phytopatholgy 159(7):488-494.
Dzung NA, Phuong VT and Dzung TT, 2011. Research on impact of chitosan oligomers on biophysical characteristics, growth, development and drought resistance of coffee. Carbohydrate Polymers 84:751755.
Erb M and Kliebenstein DJ, 2020. Plant secondary metabolites as defenses, regulators, and primary metabolites: The blurred functional trichotomy. Plant Physiology 184(1):39–52.
Esmailzadeh behabadi SV and Sharifi M, 2013. Increasing the production of plant secondary metabolites using bio-electrics. Journal of Cell and Tissue 119-128. (In Persian).
Godo T, Matsui K, Kida T, and Mii M, 1996. Effect of sugar type on the efficiency of plant regeneration from protoplasts isolated from shoot tip-derived meristematic nodular cell clumps of Lilium x formolongi hort. Plant Cell Reports 15: 401-404.
Gornik K, Grzesik M and Romanowska Duda B, 2008. The effect of chitosan on rooting of grapevine cutting and on subsequent plant growth under drought and temperature stress. Journal of Fruit Ornamental Plant Research 16: 333-343.
Guan YJ, Hu J, Wang XJ and Shao CX, 2009. Seed priming with chitosan improves maize stress germination and seedling growth in relation to physiological changes under low temperature. Journal of Zhejiang University-Science- A 10: 427-433.
Han BH, Yu HJ, Yae BW and Peak KY, 2004. In vitro micropropagation of Lilium longiflorum ‘Georgia’by shoot formation as influenced by addition of liquid medium. Scientia Horticurea 103(1), 3949.
Hasandokht M and Ebrahimi R, 2006. Principle of plant tissue culture. Marze danesh pub. Press.
Haw SG, 1986. The history of lilies in China. In: Haw S.G., editor. The Lilies of China. Timber Press; London, UK: pp. 43–56.
Heng Y, Xavier CF, Lars PC and Kai G, 2012. Chitosan Oligosaccharides Promote the Content of Polyphenols in Greek Oregano (Origanum vulgare ssp. hirtum). Journal of Agriculture and Food Chemistry 60:136-143.
Jami S, 2018. The effect of chitosan on micropropagation, secondary metabolites and antioxidant activity of selvia lerifolia Benth. M.Sc. Thesis. University of Zabol. (In Persian).
Khalafi M, Pourbeyrami Hir Y, Chamani E and Maleki Lajayer H, 2021. The Effect of Chitosan on Regeneration and Secondary Metabolite Production of Two Species of Lily Flower. Journal of Plant Research (Iranian Journal of Biology). Accepted Manuscript, Available Online from 07 March.
Kong Y, Bai J, Lang L, Bao F, Dou X, Wang H, and Shang H, 2017. Floral scents produced by Lilium and Cardiocrinum species native to China. Biochemical Systematics and Ecology 70: 222-229.
Kong Y, Wang H, Lang L, Dou X and Bai J, 2021. Metabolome-Based Discrimination Analysis of Five Lilium Bulbs Associated with Differences in Secondary Metabolites 26(5): 1340.
Kovacik J, Backor M, Strnad M and Repcak M, 2009. Salicylic acid-induced changes to growth and phenolic metabolism in Matricaria chamomilla plants. Plant Cell Report 28 (1): 135-143.
Kumar S, Kanwar JK and Sharma DR, 2015. In vitro propagation of Lilium: Review paper. Advances in Horticultural Science Vol. 20 (2): 181-188.
Liang S and Minoru NT, 2000. Lilium. In: Wu Z, Raven PH, and Hong D (eds.) Flora of China. Volume 24: Flagellariaceae through Marantaceae. Pp. 135-149. Scidence Press & Missouri Botanical Garden Press, Beijing, China; St. Louis, MO, USA.
Mahdavi B, ModarresSanavy SAM, Aghaalikhani M and Sharifi M, 2011. The Effect of water stress and chitosan on Germination and proline of seedling in safflower (Carthamus tinctorius L.). Journal of Plant Research 26(3): 352 - 365. (In Persian).
Mahdavi B, Modarres Sanavy SAM, Aghaalikhani M, Sharifi M and Dolatabadian A, 2011. Chitosan improves osmotic potential tolerance in safflower (Carthamus tinctorius L.) seedlings. Journal of Crop Improvement 25 (6): 728–741.
Malekpoor F, Salimi A and Ghasemi Pirbalouti A, 2017. The Effect of bio-elicitor of chitosan on physiological and morphological properties in purpule basil (Ocimum basilicum L.) under water deficit. Journal of Plant Ecophysiology 8(27): 56-71. (In Persian).
Mandal S, 2010. Induction of phenolics, lignin and key defense enzymes in eggplant (Solanum melongena L.) roots in response to elicitors. African Journal of Biotechnology 9 (47):8038-8047.
Michalak A, 2006. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies 15(4):523–530.
Mondal MA, Malek MA, Puteh AB, Ismail MR, Ashrafuzzaman M and Naher L, 2012. Effect of foliar application of chitosan on growth and yield in okra. Australian Journal of Crop Science 6 (5): 918-921.
No HK, Park NY, Lee SH and Meyers SP, 2002. Antibacterial activity of chitosan and chitosan oligomers with different molecular weights. International Journal of Food Microbiology 74(1-2): 65–72.
Nourafkan H, 2018. The effect of chitosan on physiological and morphological traits (lippie citriodora L.) under in-field and in vitro conditions. Journal of Crop Ecophysiology Vol.1 (49): 73-86. (In Persian).
Palida S, Rath P and Supachitra C, 2014. Chitosan increased phenolic compound contents in tea (Camellia sinensis) leaves by pre- and post-treatments. Journal of Chitin and Chitosan Science 2 (2): 93- 98.
Salachna P and Zawadzińska A, 2014. Effect of chitosan on plant growth, flowering and corms yield of potted freesia. Journal of Ecological Engineering, 15(3): 97– 102.
Sharma GK, 2015. General Techniques of Plant Tissue Culture. Lulu Press Inc. Raleigh, North Carolina, United States. ISBN; 978-1-329-73251-3.
Sheikha SAAK and AL-Malki FM, 2009. Growth and chlorophyll Responses of Bean Plants to the Chitosan Applications. European Journal of Scientific Research 50 (1): 124-134.
Singla R and Gary N, 2005. Influence of salinity on growth and yield attributes in chickpea cultivars. Turkish Journal of Agriculture and Forestry 29 (4): 231-235.
Slinkard K and Singleton VL, 1977. Total Phenol Analysis: Automation and Comparison with Manual Methods. American Journal of Enology and Viticulture 28: 49-55.
Vasyukova NI, Zinovèva SV, ilìnskaya LI, Perekhod EA, Chalenko GI, Gerasimova G, Il’ina V, Varlamov P and Ozeretskovskaya L, 2001. Modula-tion of plant resistance to diseases by water-soluble chitosan. Applied Biochemistry and Microbiology 37(1): 103-109.
Yokota S and Yahara T, 2012. Pollination biology of Lilium japonicum var. abeanum and var. japonicum: evidence of adaptation to the different availability of diurnal and nocturnal pollinators. Plant Species Biology 27(1): 96-105.
Zhao J and Sakai K, 2003. Multiple signaling pathways mediate fungal elicitor induced beta-thujaplicin biosynthesis in Cupressus lusitanica cell cultures. Journal of Experimental Botany 54(383): 647-656.
| ||
|
آمار تعداد مشاهده مقاله: 318 تعداد دریافت فایل اصل مقاله: 262 |
||