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اثر چند باکتری پروبیوتیک و روش های مختلف کاربرد آنها در کنترل زیستی بیماری برق زدگی نخود | ||
پژوهش های کاربردی در گیاهپزشکی | ||
دوره 11، شماره 1، فروردین 1401، صفحه 97-108 اصل مقاله (997.7 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22034/arpp.2021.13600 | ||
نویسندگان | ||
ناهید معرف زاده* ؛ هادی خاطری؛ روح اله شریفی | ||
گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران. | ||
چکیده | ||
چکیده بیماری برقزدگی نخود ناشی از قارچAscochyta rabiei در سراسر جهان سبب کاهش عملکرد و کیفیت نخود (Cicer arietinum) میشود. در این پژوهش، پتانسیل بیوکنترلی ۱۲ سویه باکتری پروبیوتیک از جنسهای Alcaligenes، Arthrobacter، Bacillus، Delftia، Lysinibacillus، Pseudomonas و Stenotrophomonas علیه A. rabiei بررسی گردید. در آزمونهای کشت متقابل و تولید ترکیبات فرار، همه باکتریهای پروبیوتیک (بهجز یک جدایه باسیلوس) اثر بازدارندگی معنیداری بر رشد میسلیوم بیمارگر داشتند. بیشترین بازدارندگی در آزمون کشت متقابل توسط Bacillus subtilis BS )۵۷.۸۴ درصد) و در آزمون ترکیبات فرار توسط Alcaligenes faecalis 1624 (۷۲.۵ درصد) بهدست آمد. شش باکتری پروبیوتیک با بهترین نتایج بازدارندگی در آزمایشگاه انتخاب شدند و اثر بیوکنترلی بر بیماری و تحریککنندگی رشد گیاه (وزن تر و خشک) در حضور بیمارگر با دو روش کاربرد بذری و محلولپاشی برگی، در گلخانه ارزیابی گردید. همه باکتریهای پروبیوتیک منتخب در هر دو روش کاربرد، با اختلاف معنیدار نسبت به شاهد بیمار سبب کاهش شاخص بیماری و افزایش فاکتورهای رشدی نخود شدند ولی با تیمار بذری A. faecalis 1624 افزایش وزن تر اندام هوایی مشاهده نشد. تیمار برگیB. subtilis BS با اختلاف زیاد نسبت به تیمارهای دیگر، کارآمدترین سویه در کاهش شاخص بیماری (۶۳.۸۰ درصد) و افزایش فاکتورهای رشدی گیاه نخود بود. بر طبق نتایج این تحقیق، کاربرد برگیB. subtilis BS پتانسیل بالایی در بیوکنترل برقزدگی و افزایش رشد نخود داشت. بنابراین، در صورت تأیید کارایی آن در مطالعات مزرعهای، در آینده میتوان از آن در مدیریت تلفیقی این بیماری استفاده نمود. | ||
کلیدواژهها | ||
کلمات کلیدی: کشت متقابل؛ ترکیبات فرار؛ برقزدگی نخود؛ آغشتهسازی بذر؛ پاشش برگی | ||
مراجع | ||
Refferences
Ahmad I, Jameel S, Fatima K, 2020. Assessment and management of microbial pathogens associated with chickpea (Cicer arietinum L.) grains. The International Journal of Biological Research 3 (4): 1–12. Ahmad S, Khan MA, Ahmad I, Iqbal Z, Ashraf E, et al., 2021. Efficacy of fungicides, plant extracts and biocontrol agents against Ascochyta blight (Ascochyta rabiei) of chickpea (Cicer arietinum L.) under field conditions. Plant Science Today 8 (2): 255–262. Andrews JH, 1992. Biological control in the phyllosphere. Annual Review of Phytopathology 30: 603–635. Ardalan A, Abbasi S, Sharifi R, 2017. Effect of some mineral elements on biocontrol efficiency of Bacillus pumilus INR7 against bean damping-off caused by Rhizoctonia solani. Biological Control of Pests and Plant Diseases 6 (2): 187–195 (in Persian with English abstract). Azizpour N, Rouhrazi K, 2016. Isolation and characterization of rhizosphere bacteria for the biocontrol of the Ascochyta rabiei in Iran. Advances in Plants and Agriculture Research 3(4): 121–125. Bahr L, Castelli MV, Barolo MI, Ruiz Mostacero N, Tosello ME, et al., 2016. Ascochyta blight: isolation, characterization, and development of a rapid method to detect inhibitors of the chickpea fungal pathogen Ascochyta rabiei. Fungal Biology 120 (3): 424–432. Bai JY, Wang DY, Li HJ, Wang XM, 2011. First report of Ascochyta rabiei causing ascochyta blight of Cicer arietinum in China. Journal of Plant Pathology 93 (4): S83. Baite MS, Dubey SC, 2018. Pathogenic variability of Ascochyta rabiei causing blight of chickpea in India. Physiological and Molecular Plant Pathology 102: 122–127. Benzohra EE, Bendahmane BS, Labdi M, Benkada MY, 2012. Determination of pathotypes and physiological races in Ascochyta rabiei, the agent of ascochyta blight in chickpea (Cicer arietinum L.) in Algeria. African Journal of Agricultural Reseearch 7(7): 1214–1219. Benzohra IE, Bendahmane BS, Labdi M, Bnekada MY, 2013. In vitro biocontrol using the antagonist Trichoderma harzianum against the algerian isolates of Ascochyta rabiei (Pass.) Labr., the agent of Ascochyta blight in chickpea (Cicer arietinum L.). International Journal of Microbiological Research 2 (2): 124–128. Benzohra IE, Bendahmane BS, Benkada MY, Mégateli M, Belaidi H, 2020. Use of three synthetic fungicides to reduce the incidence of Ascochyta blight (Ascochyta rabiei) in chickpea (Cicer arietinum L.): A susceptible cultivars case. Indian Journal of Agricultural Research 54: 459–464. Borzouei S, Sharifi R, Moarrefzadeh N, 2019. Induction of systemic resistance in tomato against broomrape (Phelipanche aegyptiaca). Journal of Phytopathology 167 (10): 567–575. Chen W, Coyne CJ, Peever TL, J. Muehlbauer F, 2004. Characterization of chickpea differentials for pathogenicity assay of ascochyta blight and identification of chickpea accessions resistant to Didymella rabiei. Plant Pathology 53 (6): 759–769. Contreras-Cornejo HA, Macias-Rodriguez L, Beltran-Pena E, Herrera-Estrella A, Lopez-Bucio J, 2011. Trichoderma-induced plant immunity likely involves both hormonal- and camalexin-dependent mechanisms in Arabidopsis thaliana and confers resistance against necrotrophic fungi Botrytis cinerea. Plant Signaling and Behavior 6 (10): 1554–63. Dennis C, Webster J, 1971. Antagonistic properties of species-groups of Trichoderma: I. Production of non-volatile antibiotics. Transactions of the British Mycological Society 57 (1): 41–48. Efthimiadou A, Katsenios N, Chanioti S, Giannoglou M, Djordjevic N, et al., 2020. Effect of foliar and soil application of plant growth promoting bacteria on growth, physiology, yield and seed quality of maize under Mediterranean conditions. Scientific Reports 10 (1): 21060. Ennouri A, Lamiri A, Essahli M, Bencheqroun SK, 2020. Chemical composition of essential oils and their antifungal activity in controlling Ascochyta rabiei. Journal of Agricultural Science and Technology 22 (5): 1371–1381. Farace G, Fernandez O, Jacquens L, Coutte F, Krier F, et al., 2015. Cyclic lipopeptides from Bacillus subtilis activate distinct patterns of defence responses in grapevine. Molecular Plant Pathology 16 (2): 177–187. Farahani S, Talebi R, Maleki M, Mehrabi R, Kanouni H, 2019. Pathogenic diversity of Ascochyta rabiei isolates and identification of resistance sources in core collection of chickpea germplasm. The Plant Pathology Journal 35 (4): 321–329. Gan Y, Warkentin TD, Chandirasekaran R, Gossen BD, Wolf T, et al., 2009. Effects of planting pattern and fungicide application systems on Ascochyta blight control and seed yield in chickpea. Agronomy Journal 101 (6): 1548–1555. Garbeva P, Van Elsas J, Van Veen J, 2008. Rhizosphere microbial community and its response to plant species and soil history. Plant and Soil 302 (1–2): 19–32. Ghorbani M, Sabagh SK, Karimi A, 2021. Biological control of fusarium wilt of chickpea using Rhizophagus irregularis fungus and nitroxin bio-fertilizer. Journal of Applied Research in Plant Protection 10 (4): 61–70. Han J, Sun L, Dong X, Cai Z, Sun X, et al., 2005. Characterization of a novel plant growth-promoting bacteria strain Delftia tsuruhatensis HR4 both as a diazotroph and a potential biocontrol agent against various plant pathogens. Systematic and Applied Microbiology 28 (1): 66–76. Huang Y, He Y, Ye B-C, Li C, 2017. Rhizospheric Bacillus subtilis exhibits biocontrol effect against Rhizoctonia solani in pepper (Capsicum annuum). BioMed Research International 2017: 1–9. Jamshidipoor S, Vafaei SH, 2021. Impact of the virulence and spore concentration of Didymella rabiei isolates on the reaction of chickpea genotypes to ascochyta blight. Journal of Applied Research in Plant Protection 10 (3): 63–71 (in Persian with English abstract). Javaid A, Munir R, Khan IH, Shoaib A, 2020. Control of the chickpea blight, Ascochyta rabiei, with the weed plant, Withania somnifera. Egyptian Journal of Biological Pest Control 30 (1): 1–8. Jayakumar P, Gan YT, Gossen BD, Warkentin TD, Banniza S, 2005. Ascochyta blight of chickpea: infection and host resistance mechanisms. Canadian Journal of Plant Pathology 27 (4): 499–509. Khabbaz SE, Ladhalakshmi D, Babu M, Kandan A, Ramamoorthy V, et al., 2019. Plant growth promoting bacteria (PGPB) – A versatile tool for plant health management. Canadian Journal of Pesticides and Pest Management 1 (1): 1–25. Küçük Ç, Kivanç M, Kinaci E, Kinaci G, 2007. Efficacy of Trichoderma harzianum (Rifaii) on inhibition of ascochyta blight disease of chickpea. Annals of Microbiology 57 (4): 665–668. Kumbar B, Mahmood R, Nagesha SN, Nagaraja MS, Prashant DG, et al., 2019. Field application of Bacillus subtilis isolates for controlling late blight disease of potato caused by Phytophthora infestans. Biocatalysis and Agricultural Biotechnology 22. Legein M, Smets W, Vandenheuvel D, Eilers T, Muyshondt B, et al., 2020. Modes of action of microbial biocontrol in the phyllosphere. Frontiers in Microbiology 11. Liu N, Xu S, Yao X, Zhang G, Mao W, et al., 2016. Studies on the control of Ascochyta blight in field peas (Pisum sativum L.) caused by Ascochyta pinodes in Zhejiang Province, China. Frontiers in Microbiology 7: 481. Manjunatha L, Saabale PR, Srivastava AK, Dixit GP, Yadav LB, et al., 2018. Present status on variability and management of Ascochyta rabiei infecting chickpea. Indian Phytopathology 71 (1): 9–24. Moarrefzadeh N, Sharifi R, Khateri H, Abbasi S, 2021a. Effect of some probiotics consortia on inhibition of Fusarium yellowing and wilting disease (Fusarium redolens Wollenweber) and growth promoting in chickpeas. Journal of Agricultural Science and Sustainable Production 31 (4): 255–270. Moarrefzadeh N, Sharifi R, Khateri H, Abbasi S, 2021b. Biological control of Fusarium oxysporum f. sp. ciceris, the causal agent of the fusarial yellowing and wilting of chickpea by mixtures of some microbial agents. Biological Control of Pests and Plant Diseases 9 (1): 61–73. Pande S, Siddique KHM, Kishore GK, Bayaa B, Gaur PM, et al., 2005. Ascochyta blight of chickpea (Cicer arietinum L.): a review of biology, pathogenicity, and disease management. Australian Journal of Agricultural Research 56 (4): 317–332. Pande S, Sharma M, Gaur PM, Tripathi S, Kaur L, et al., 2011. Development of screening techniques and identification of new sources of resistance to Ascochyta blight disease of chickpea. Australasian Plant Pathology 40 (2): 149–156. Parida SK, Gayacharan, Rani U, Singh S, Basandrai AK, et al., 2020. Identification of novel resistant sources for ascochyta blight (Ascochyta rabiei) in chickpea. PLoS One 15 (10): e0240589. Parmasi Z, Tahmasebi Z, Zare MJ, Nourollahi K, Kanouni H, 2019. Biocontrol of Ascochyta blight by Azospirillum sp. depending on the degree of resistance of chickpea genotypes. Journal of Phytopathology 167 (10): 601–607. Rajakumar E, Aggarwal R, Singh B, 2005. Fungal antagonists for the biological control of Ascochyta blight of chickpea. Acta Phytopathologica et Entomologica Hungarica 40 (1–2): 35–42. Ryu CM, Farag MA, Hu CH, Reddy MS, Kloepper JW, et al., 2004. Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiology 134(3): 1017–26. Sangiogo M, Rodriguez DP, Moccellin R, Bermudez JMM, Corrêa BO, et al., 2018. Foliar spraying with bacterial biocontrol agents for the control of common bacterial blight of bean. Pesquisa Agropecuária Brasileira 53 (10): 1101–1108. Seifi S, 2019. Screening of nitrate removal bacteria and effect of these bacteria on biological control of Fusarium wilt disease in tomato. PhD thesis, University of Tehran, Karaj, Iran.
Seifi S, Behboudi K, Sharifi R, Shapleigh JP, 2020. Introduction, mechanisms of action and genomic description in plant probiotic bacterium Bacillus velezensis. Iranian Journal of Plant Protection Science 50 (2): 159–175 (in Persian with English abstract). Sharifi R, Ryu CM, 2016. Making healthier or killing enemies? Bacterial volatile-elicited plant immunity plays major role upon protection of Arabidopsis than the direct pathogen inhibition. Communicative and Integrative Biology 9(4): e1197445. Sharma M, Pande S, Rathore A, 2010. Effect of growth stages of chickpea on the genetic resistance of Ascochyta blight. European Journal of Plant Pathology 128(3): 325–331. Tadesse M, Turoop L, Ojiewo CO, 2017. Survey of chickpea Cicer arietinum L) Ascochyta blight (Ascochyta rabiei Pass.) disease status in production regions of Ethiopia. Plant 5(1): 23. Tahir HAS, Gu Q, Wu H, Raza W, Hanif A, et al., 2017. Plant growth promotion by volatile organic compounds produced by Bacillus subtilis SYST2. Frontiers in Microbiology 8: 171. Tsegaye Z, Assefa F, Tefera G, Alemu T, Gizaw B, et al., 2018. Concept, principle and application of biological control and their role in sustainable plant diseases management strategies. International Journal of Research Studies in Biosciences 6 (4): 18–34. Vafaei S, Rezaee S, Moghadam AA, Zamanizadeh H, 2017. Screening of Chickpea germ plasms for selection of resistant genotypes to Ascochyta blight. Applied Entomology and Phytopathology 85(1): 97–110 (in Persian with English abstract). Viani A, Pouralibaba HR, Abolfathzadeh S, 2021. Effect of lentil seed priming as hydropriming, biopriming and with resistance inducing materials in management of Fusarium wilt disease under laboratory, glasshouse and field conditions. Journal of Applied Research in Plant Protection 10 (4): 47–59. Wang H, Hwang SF, Chang KF, Turnbull GD, Howard RJ, 2003. Suppression of important pea diseases by bacterial antagonists. BioControl 48 (4): 447–460. Zerroug MM, Bouzid D, Mezaache S, 2011. Effect of Bacillus megaterium filtrates on the growth and spore germination of Ascochyta rabiei. In: AFPP –Quatrième Conférence Internationale Sur Les Méthodes Alternatives En Protection Des Cultures, March 8–10, Lille, France. P. 634–637. Zhang L, Khabbaz S, Wang A, Li H, Abbasi P, 2015. Detection and characterization of broad‐spectrum antipathogen activity of novel rhizobacterial isolates and suppression of Fusarium crown and root rot disease of tomato. Journal of Applied Microbiology 118 (3): 685–703. | ||
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