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اثر استفاده ازسطوح مختلف تانیک اسید در شرایط آزمایشگاهی بر فراسنجههای تخمیر و الگوی اسیدهای چرب مایع شکمبه | ||
پژوهش های علوم دامی (دانش کشاورزی) | ||
دوره 31، شماره 1، خرداد 1400، صفحه 37-51 اصل مقاله (1.03 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22034/as.2021.37905.1546 | ||
نویسندگان | ||
فرشید فتاح نیا* 1؛ علی خطیب جو2؛ حمید رضا میرزایی الموتی3؛ لادن رشیدی4؛ نسیم رحیمی5؛ صفورا یوسفی نژاد6 | ||
1هیئت علمی گروه علوم دامی دانشگاه ایلام | ||
2دانشگاه ایلام | ||
3عضو هیئت علمی گروه علوم دامی زنجان | ||
4اداره کل استاندارد و تحقیقات صنعتی کشور | ||
5دانش آموخته کارشناسی ارشد دانشگاه ایلام | ||
6دانشجوی کارشناسی ارشد | ||
چکیده | ||
زمینه مطالعاتی: تانیک اسید (یک نوع تانن قابل هیدرولیز) قابلیت تغییر فرایندهای تخمیری شکمبه و مسیر بیوهیدروژناسیون اسیدهای چرب غیراشباع را دارد. هدف: این آزمایش به منظور بررسی اثر سطوح مختلف تانیک اسید بر پارامترهای تخمیری شکمبهای در شرایط آزمایشگاه و حدواسطهای بیوهیدروژناسیون اسیدهای چرب غیراشباع انجام گرفت. روش کار: جیره پایه حاوی نسبت کنسانتره به علوفه 2/58 به 8/41 و دانه سویای اکسترود شده به عنوان منبع اسیدهای چرب غیر اشباع بود. تیمارهای آزمایشی شامل کنترل (بدون افزودنی)، 1/0 میلیگرم موننسین در لیتر محیط کشت، 250 میلیگرم تانیک اسید در لیتر محیط کشت، 500 میلیگرم تانیک اسید در لیتر محیط کشت و 750 میلیگرم تانیک اسید در لیتر محیط کشت بودند. تولید گاز، غلظت آمونیاک و اسیدهای چرب فرار مایع شکمبه و الگوی اسیدهای چرب مایع شکمبه اندازهگیری شد. نتایج: تولید گاز، pH و غلظت نیتروژن آمونیاکی و اسیدهای چرب فرار مایع شکمبه تحت تاثیر افزودنی قرار نگرفت (05/0< p). تانیک اسید غلظت اسیدهای چرب 10:0 تا 14:0 در مایع کشت را کاهش و غلظت 16:0 را افزایش داد (05/0>P). افزودن موننسین و 250 میلیگرم تانیک اسید در لیتر محیط کشت مقدار 18:0 را کاهش داد (05/0>P). موننسین مقدار 18:1 ترانس-11 (ترانس واکسنیک اسید) و 18:2 سیس-9، ترانس-11 (اسید لینولئیک کونژوگه) را در مقایسه با سایر تیمارها افزایش داد (05/0>P). افزودن 500 میلیگرم تانیک اسید در لیتر محیط کشت غلظت اسیدهای چرب غیراشباع و اسیدهای چرب بلند زنجیر را افزایش داد (05/0>P). نتیجهگیری نهایی: در کل، استفاده از اسید تانیک در زمان تغذیه جیره حاوی دانه سویای اکسترود شده با تغییر بیوهیدروژناسیون اسیدهای چرب غیراشباع باعث افزایش اسیدهای چرب غیراشباع و اسیدهای چرب مفید برای سلامت انسان شد. | ||
کلیدواژهها | ||
تانیک اسید؛ تخمیر شکمبه؛ دانه سویای اکسترود شده؛ بیوهیدروژناسیون اسیدهای چرب غیراشباع | ||
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