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A key response of grain yield and superoxide dismutase in maize (Zea mays L.) to water deficit stress | ||
Journal of Plant Physiology and Breeding | ||
مقاله 8، دوره 9، شماره 2، اسفند 2019، صفحه 77-84 اصل مقاله (424.68 K) | ||
نوع مقاله: Research Paper | ||
شناسه دیجیتال (DOI): 10.22034/jppb.2019.10606 | ||
نویسندگان | ||
Sajjad Moharramnejad* 1؛ Mostafa Valizadeh2 | ||
1Crop and Horticultural Research Department, Ardabil Agricultural and Natural Resources Research and Education Center, AREEO, Moghan, Iran. | ||
2Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. | ||
چکیده | ||
To assess the grain yield, plant height and superoxide dismutase (Cu/Zn-SOD) activity, and its expression responses of maize to water deficit stress, a field experiment using a split-plot design based on randomized complete block design was performed with three maize hybrids, SC704, SC720 and NS640, under control and water-deficit stress conditions at the Research Station of University of Tabriz, Iran. The results indicated that water deficit stress reduced grain yield and plant height. Electrophoretic analysis for Cu/Zn-SOD based on sensitivity to KCN and H2O2 inhibitors was carried out using 8% slab polyacrylamide gels. The gene expression of Cu/Zn-SOD by using Real-time PCR in maize hybrids showed that water deficit stress increased Cu/Zn-SOD expression. SC704 with higher grain yield, plant height and Cu/Zn-SOD activity ranked as a drought-tolerant hybrid in this study. It can be concluded that the increase in Cu/Zn-SOD expression may decrease damage caused by water-deficit stress in maize. | ||
کلیدواژهها | ||
Antioxidant enzyme؛ Electrophoresis؛ Gene؛ Grain yield؛ Isoform؛ Plant height | ||
مراجع | ||
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