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Salinity stress responsive leaf proteins in alfalfa (Medicago sativa L.) | ||
| Journal of Plant Physiology and Breeding | ||
| مقاله 12، دوره 9، شماره 1، شهریور 2019، صفحه 147-157 اصل مقاله (616.23 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2019.10403 | ||
| نویسندگان | ||
| Zahra Dehghanian1؛ Ali Bandehagh* 1؛ Adel Dabbagh Mohammadi Nasab2 | ||
| 1Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. | ||
| 2Department of Plant Ecophysiology, Faculty of Agriculture University of Tabriz, Tabriz, Iran. | ||
| چکیده | ||
| Salinity stress is one of the most harmful abiotic stresses that affect agronomical, physiological and biochemical processes in crop plants. In order to evaluate the effect of salinity stress on alfalfa (Bami ecotype) leaf proteins based on two-dimensional gel electrophoresis, an experiment was conducted with two salinity treatments (0 and 200 mM NaCl) and six replications under hydroponic cultural system. Salinity stress decreased fresh and dry weight of alfalfa about 24.5 and 39.5 percent, respectively. Proteome analysis was carried out on the leaf tissue using three replications. Fourteen repeatable protein spots had significant change in expression under salt stress. Probabilistic identification of proteins from the data bank was performed by isoelectric point and molecular weight. Nine proteins showed significant up-regulation and five proteins had down regulation. Candidate proteins were among the proteins involved in the defense system, regulation, metabolic pathways, nitrogen fixation and canalization. Higher abundance of trehalose-phosphate phosphatase 2, involved in regulation, and also uracil phosphoribosyl transferase lastic and β-hydroxyisobutyryl-CoA hydrolase 1, involved in the energy metabolism, demonstrated the important function of these proteins under salinity stress. It seems that the alfalfa plant manages to reduce the adverse effects of salinity through the candidate proteins identified in this investigation. | ||
| کلیدواژهها | ||
| Abiotic stress؛ Defense system؛ Metabolism؛ Proteomics؛ Two-DE gel | ||
| مراجع | ||
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Anonymous, 2019. Global Yield Gap Atlas. http://www.yieldgap.org/Iran. Ashraf M, 2009. Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnology Advances 27(1): 84-93. Ashraf M and Harris PJC, 2013. Photosynthesis under stressful environments: an overview. Photosynthetica 51: 163-190. Bohnert HJ, Nelson DE and Jensen RG, 1995. Adaptations to environmental stresses. The Plant Cell 7(7): 1099-1111.
Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254. Brill EM, Abrahams S, Hayes CM, Jenkins CLD and Watson JM, 1999. Molecular characterisation and expression of a wound-inducible cDNA encoding a novel cinnamyl-alcohol dehydrogenase enzyme in lucerne (Medicago sativa L.). Plant Molecular Biology 41: 279-291. Eilers RG, Eilers WD and Fitzgerald MM, 1997. A salinity risk index for soils of the Canadian prairies. Hydrogeology Journal 5(1): 68-79.
Farissi M, Faghire M, Bargaz A, Bouizgaren A, Makoudi B, Sentenac H and Ghoulam C, 2014. Growth, nutrients concentrations and enzymes involved in plants nutrition of alfalfa populations under saline conditions. Journal of Agricultural Science and Technology 16: 301-314. Gao L, Yan X, Li X, Guo G, Hu Y, Ma W and Yan Y 2011. Proteome analysis of wheat leaf under salt stress by two-dimensional difference gel electrophoresis (2D-DIGE). Phytochemistry 72(10): 1180-1191.
Gill SS and Tuteja N, 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48(12): 909-930. Hoagland DR and Arnon DI 1950. The water-culture method for growing plants without soil. Circular 347. California Agricultural Experiment Station. The College of Agriculture University of California, Berkeley, USA. Höper D, Bernhardt J and Hecker M, 2006. Salt stress adaptation of Bacillus subtilis: a physiological proteomics approach. Proteomics6: 1550-1562. Karbassi A, Nabi Bidhendi G, Pejman A and Esmaeili Bidhendi M, 2010. Environmental impacts of desalination on the ecology of Lake Urmia. Journal of Great Lakes Research 36(3): 419-424. MA Q-L, Kang J-M, Long R-C, Cui Y-J, Zhang T-J, Xiong J-B, Yang Q-C and Yan S, 2016. Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings. Journal of Integrative Agriculture 15(10): 2266-2278. Mainguet SE, Gakière B, Majira A, Pelletier S, Bringel F, Guérard F, Caboche M, Berthomé R and Renou JP, 2009. Uracil salvage is necessary for early Arabidopsis development. The Plant Journal 60(2): 280-291. Marino D, Damiani I, Gucciardo S, Mijangos I, Pauly N and Puppo A, 2013. Inhibition of nitrogen fixation in symbiotic Medicago truncatula upon Cd exposure is a local process involving leghaemoglobin. Journal of Experimental Botany 64(18): 5651-5660. Marschner H, 1995.Mineral Nutrition of Higher Plants. Second edition. Academic Press, London.
Michaud R, Lehman WF and Rumbaugh MD, 1988. World distribution and historical development. In: Hanson AA, Barnes DK. and Hill Jr. RR (eds.) Alfalfa and Alfalfa Improvement. Pp. 25-91. Agronomy Monographs, ASSA, CSSA, SSSA, USA. Mittler R, 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7(9): 405-410. Munns RA, 1992. A leaf elongation assay detects an unknown growth inhibitor in xylem sap from wheat and barley. Australian Journal of Plant Physiology19(2): 127-135. Munns R and Tester M, 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology 59: 651-681.
Nakashima K, Takasaki H, Mizoi J, Shinozaki K and Yamaguchi-Shinozaki K, 2012. NAC transcription factors in plant abiotic stress responses. Biochimica et Biophysica Acta 1819: 97-103.
Park J-I, Ahmed NU, Jung H-J, Arasan SKT, Chung M-Y, Cho Y-G, Watanabe M and Nou I-S, 2014. Identification and characterization of LIM gene family in Brassica rapa. BMC Genomics 15: 641. https://doi.org/10.1186/1471-2164-15-641. Pavoković D, Križnik B and Krsnik-Rasol M, 2012. Evaluation of protein extraction methods for proteomic analysis of non-model recalcitrant plant tissues. Croatica Chmica Acta 85(2): 177-183. Percey WJ, Shabala L, Wu Q, Su N, Breadmore MC, Guijt RM, Bose J and Shabala S, 2016. Potassium retention in leaf mesophyll as an element of salinity tissue tolerance in halophytes. Plant Physiology and Biochemistry 109: 346-354. Sachs MM and Ho THD 1986. Alteration of gene expression during environmental stress in plants. Annual Review of Plant Physiology 37(1): 363-376.
Seki M, Kamei A, Satou M, Sakurai T, Fujita M, Oono Y, Yamaguchi-Shinozaki K and Shinozaki K, 2003. Transcriptome analysis in abiotic stress conditions in higher plants. In: Hirt H and Shinozaki K (eds.) Plant Responses to Abiotic Stress. Topics in Current Genetics. Vol. 4. Pp. 271-308. Springer, Berlin, Heidelberg. Srivastava V and Verma PK, 2015. The plant LIM proteins: unlocking the hidden attractions. Planta 246(3): 365-375. Valizadeh M, Moharamnejad S, Ahmadi M and Mohammadzadeh Jalaly H, 2013. Changes in activity profile of some antioxidant enzymes in alfalfa half-sib families under salt stress. Journal of Agricultural Science and Technology 15(4): 801-809. Valizadeh M, Mohayeji M, Yasinzadeh N, Nasrullazadeh S and Moghaddam M, 2011. Genetic diversity of synthetic alfalfa generations and cultivars using tetrasomic inherited allozyme markers. Journal of Agricultural Science and Technology 13: 425-430. Xiong J, Sun Y, Yang Q, Tian H, Zhang H, Liu Y and Chen M 2017. Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots. Proteome Science 15: 19. https://doi.org/10.1186/s12953-017-0127-z. Yacoubi R, Job C, Belghazi M, Chaibi W and Job D, 2013. Proteomic analysis of the enhancement of seed vigour in osmoprimed alfalfa seeds germinated under salinity stress. Seed Science Research 23(2): 99-110. Zeng N, Yang Z, Zhang Z, Hu L and Chen L, 2019. Comparative transcriptome combined with proteome analyses revealed key factors involved in alfalfa (Medicago sativa) response to waterlogging stress. Zhang L, Kars I, Essenstam B, Liebrand TW, Wagemakers L, Elberse J, Tagkalaki P, Tjoitang D, van den Ackerveken G and van Kan JA, 2014. Fungal endopolygalacturonases are recognized as microbe-associated molecular patterns by the Arabidopsis receptor-like protein. Plant Physiology 164(1): 352-364. Zolman BK, Monroe-Augustus M, Thompson B, Hawes JW, Krukenberg KA, Matsuda SP and Bartel B, 2001. chy1, an Arabidopsis mutant with impaired β-oxidation, is defective in a peroxisomal β-hydroxyisobutyryl-CoA hydrolase. Journal of Biological Chemistry 276(33): 31037-31046. | ||
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