|تعداد مشاهده مقاله||49,944,435|
|تعداد دریافت فایل اصل مقاله||13,157,563|
Proteomics analysis of canola seeds to identify differentially expressed proteins under salt stress
|Journal of Plant Physiology and Breeding|
|مقاله 7، دوره 9، شماره 1، شهریور 2019، صفحه 83-95 اصل مقاله (635.72 K)|
|نوع مقاله: Research Paper|
|شناسه دیجیتال (DOI): 10.22034/jppb.2019.10386|
|Samaneh Eyvazlou؛ Ali Bandehagh* ؛ Majid Norouzi؛ Mahmoud Toorchi؛ Reza Shokri Gharelo|
|Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.|
|Seeds are an important part of the canola plant, and similar to other parts are affected by salt stress. Understanding the underlying mechanisms that take place in seeds of canola under salt stress is essential from the perspective of improving quality. In this study, we attempted to identify differentially expressed proteins of canola seeds in the Hyola308 cultivar under 350 mM NaCl using two dimensional (2D) gel electrophoresis. Effect of salt stress was significant on 100-seed weight and number of seeds per plant (p≤ 0.01), and it changed the proteome of the seeds. From a total of 548 reproducible protein spots, 28 protein spots showed significant changes in abundance, of which seven spots showed downregulation. The “Gene Ontology” analysis classified differentially expressed proteins into six biological processes: oxidation-reduction (28.5%), response to abiotic stress (28.5%), response to hormones (21.4%), catabolic process (21.4%), nucleoside diphosphate phosphorylation (17.8%) and glycolytic process (14.28%). In conclusion, salt stress induced canola seeds to upregulate proteins that mostly involved in the antioxidant activity and the proteins with nutrient reservoir activity.|
|Abiotic stress؛ Antioxidant activity؛ Brassica napus؛ Proteomics؛ Two-dimensional polyacrylamide gel electrophoresis|
Aki T, Shigyo M, Nakano R, Yoneyama T and Yanagisawa S, 2008. Nano scale proteomics revealed the presence of regulatory proteins including three FT-Like proteins in phloem and xylem saps from rice. Plant and Cell Physiology 49(5): 767-790.
Araújo WL, Tohge T, Ishizaki K, Leaver CJ and Fernie AR, 2011. Protein degradation- an alternative respiratory substrate for stressed plants. Trends in Plant Science 16(9): 489-498.
Banaei-Asl F, Bandehagh A, Uliaei ED, Farajzadeh D, Sakata K, Mustafa G and Komatsu S, 2015. Proteomic analysis of canola root inoculated with bacteria under salt stress. Journal of Proteomics 124: 88-111.
Bandehagh A, Salekdeh GH, Toorchi M, Mohammadi A and Komatsu S, 2011. Comparative proteomic analysis of canola leaves under salinity stress. Proteomics 11(10): 1965-1975.
Bao J, Pan G, Poncz M, Wei J, Ran M and Zhou Z, 2018. Serpin functions in host-pathogen interactions. PeerJ 6: e4557. doi:10.7717/peerj.4557.
Battaglia M and Covarrubias AA, 2013. Late embryogenesis abundant (LEA) proteins in legumes. Frontiers in Plant Science 4: 190. doi: 10.3389/fpls.2013.00190.
Bhushan D, Pandey A, Choudhary MK, Datta A, Chakraborty S and Chakraborty N, 2007. Comparative proteomics analysis of differentially expressed proteins in chickpea extracellular matrix during dehydration stress. Molecular & Cellular Proteomics 6(11): 1868-1884.
Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
Brandão AR, Barbosa HS and Arruda MAZ, 2010. Image analysis of two-dimensional gel electrophoresis for comparative proteomics of transgenic and non-transgenic soybean seeds. Journal of Proteomics 73(8): 1433-1440.
Bykova NV, Hoehn B, Rampitsch C, Banks T, Stebbing JA, Fan T and Knox R, 2011. Redox‐sensitive proteome and antioxidant strategies in wheat seed dormancy control. Proteomics 11(5): 865-882.
Catusse J, Strub J-M, Job C, Van Dorsselaer A and Job D, 2008. Proteome-wide characterization of sugarbeet seed vigor and its tissue specific expression. PNAS 105(29): 10262-10267.
Chaves MM, Flexas J and Pinheiro C, 2009. Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany 103(4): 551-560.
Chibani K, Ali-Rachedi S, Job C, Job D, Jullien M and Grappin P, 2006. Proteomic analysis of seed dormancy in arabidopsis. Plant Physiology 142(4): 1493-1510.
Clifton R, Lister R, Parker KL, Sappl PG, Elhafez D, Millar AH, Day DA and Whelan J, 2005. Stress-induced co-expression of alternative respiratory chain components in Arabidopsis thaliana. Plant Molecular Biology 58(2): 193-212.
Douce R, 1985. Mitochondria in Higher Plants: Structure, Function, and Biogenesis. Academic Press, Inc., USA.
FAO, 2009. High level expert forum-how to feed the world in 2050. Economic and Social Development, Food and Agricultural Organization of the United Nations, Rome, Italy.
Farooq M, Wahid A, Kobayashi N, Fujita D and Basra SMA, 2009. Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development 29(185–212).
Finnie C, Melchior S, Roepstorff P and Svensson B, 2002. Proteome analysis of grain filling and seed maturation in barley. Plant Physiology 129: 1308-1319.
Gallardo K, Firnhaber C, Zuber H, Héricher D, Belghazi M, Henry C, Küster H and Thompson R, 2007. A combined proteome and transcriptome analysis of developing Medicago truncatula seeds: evidence for metabolic specialization of maternal and filial tissues. Molecular and Cellular Proteomics 6(12): 2165-2179.
Ghassemi-Golezani K, Taifeh-Noori M, Oustan Sh and Moghaddam M, 2009. Response of soybean cultivars to salinity stress. Journal of Food, Agriculture and Environment 7(2): 401-404.
Ghazani SM and Marangoni AG, 2016. Healthy fats and oils. In: Wrigley C, Corke H, Seetharaman K and Faubion J (eds.). Encyclopedia of Food Grains (Second Edition). Volume 2. Elsevier Ltd., Netherlands.
Gunstone F, 2011. Vegetable Oils in Food Technology: Composition, Properties and Uses. John Wiley & Sons.
Gupta B and Huang B, 2014. Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. International Journal of Genomics 2014:701596. doi: 10.1155/2014/701596.
Hasegawa PM, Bressan RA, Zhu JK and Bohnert HJ, 2000. Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology 51: 463-499.
Herman EM, 2014. Soybean seed proteome rebalancing. Frontiers in Plant Science 5: 437. doi.org/10.3389/fpls.2014.00437.
Katz AK, Li X, Carrell HL, Hanson BL, Langan P, Coates L, Schoenborn BP, Glusker JP and Bunick GJ. 2006. Locating active-site hydrogen atoms in D-xylose isomerase: time-of-flight neutron diffraction. Proceedings of the National Academy of Sciences 103(22): 8342-8347.
Kaya MD, Okçu G, Atak M, Cıkılı Y and Kolsarıcı Ö, 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus l.). European Journal of Agronomy 24(4): 291-295.
Konopka-Postupolska D, Clark G and Hofmann A, 2011. Structure, function and membrane interactions of plant annexins: an update. Plant Science 181(3): 230-241.
Laino P, Shelton D, Finnie C, De Leonardis AM, Mastrangelo AM, Svensson B, Lafiandra D and Masci S, 2010. Comparative proteome analysis of metabolic proteins from seeds of durum wheat (cv. Svevo) subjected to heat stress. Proteomics 10(12): 2359-2368.
Maere S, Heymans K and Kuiper M, 2005. BiNGO: A Cytoscape plugin to assess overrepresentation of Gene Ontology categories in Biological Networks. Bioinformatics 21(16): 3448-3449.
Miernyk JA and Hajduch M, 2011. Seed proteomics. Journal of Proteomics 74(4): 389-400.
Miernyk JA, Preťová A, Olmedilla A, Klubicová K, Obert B and Hajduch M, 2011. Using proteomics to study sexual reproduction in angiosperms. Sexual Plant Reproduction 24(1): 9-22.
Millar AH, Whelan J, Soole KL and Day DA, 2011. Organization and regulation of mitochondrial respiration in plants. Annual Review of Plant Biology 62: 79-104.
Moon H, Lee B, Choi G, Shin D, Prasad DT, Lee O, Kwak SS, Kim DH, Nam J, Bahk J, Hong JC, Lee SY, Cho MJ, Lim CO and Yun DJ, 2003. NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. PNAS USA 100(1): 358-363.
Munns R, 2002. Comparative physiology of salt and water stress. Plant, Cell and Environment 25(2): 239-250. Munns R and Tester M, 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology 59: 651-681.
Panteghini M and Bais R, 2012. Serum enzymes. In: Burtis CA, Ashwood ER and Bruns DE, (eds.). Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Fifth edition. Pp. 565-598. Elsevier Saunders, Missouri, USA.
Parida AK and Das AB, 2005. Salt tolerance and salinity effects on plants: a review.
Parks RE Jr and Agarwal RP, 1973. Nucleoside diphosphokinases. Enzymes 8(Part A): 307-334.
Persson B and Kallberg Y, 2013. Classification and nomenclature of the superfamily of short-chain dehydrogenases/reductases (SDRs). Chemico-Biological Interactions 202(1-3): 111-115.
Portis AR Jr, 1992. Regulation of ribulose1,5-bisphosphte carboxylase/oxygenase. Annual Review of Plant Physiology and Plant Molecular Biology 43: 415-437.
Purty RS, Kumar G, Singla-Pareek SL and Pareek A, 2008. Towards salinity tolerance in Brassica: an overview. Physiology and Molecular Biology of Plants 14(1-2): 39-49.
Rhee SG, Yang K-S, Kang SW, Woo HA and Chang T-S, 2005. Controlled elimination of intracellular H(2)O(2): regulation of peroxiredoxin, catalase, and glutathione peroxidase via post-translational modification. Antioxidants & Redox Signaling 7(5-6): 619-626.
Rutkowski A, 1971. The feed value of rapeseed meal. Journal of the American Oil Chemists'''' Society 48: 863-868.
Samac DA, Hironaka CM, Yallaly PE and Shah DM, 1990. Isolation and characterization of the genes encoding basic and acidic chitinase in Arabidopsis thaliana. Plant Physiology 93: 907-914.
Sammons DW, Adams LD and Nishizawa EE, 1981. Ultrasensitive silver‐based color staining of polypeptides in polyacrylamide gels. Electrophoresis 2: 135-141.
Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B and Ideker T, 2003. Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Research 13(11): 2498-2504.
Singh KB, Malhotra RS, Halila MH, Knights EJ and Verma MM, 1993. Current status and future strategy in breeding chickpea for resistance to biotic and abiotic stresses. Euphytica 73: 137–149.
Sirover MA, 2011. On the functional diversity of glyceraldehyde-3-phosphate dehydrogenase: biochemical mechanisms and regulatory control. Biochimica et Biophysica Acta (BBA)-General Subjects 1810(8): 741-751.
Shokri Gharelo R, Bandehagh A, Tourchi M and Farajzadeh D, 2016. Canola 2-dimensional proteom profiles under osmotic stress and inoculation with Pseudomonas fluorescens FY32. Plant Cell Biotechnology and Molecular Biology 17(5-6): 257-266.
Sreenivasulu N, Sopory S and Kavi Kishor PK, 2007. Deciphering the regulatory mechanisms of abiotic stress tolerance in plants by genomic approaches. Gene 388(1-2): 1-13.
Suzuki N, Koussevitzky S, Mittler R and Miller G, 2012. ROS and redox signalling in the response of plants to abiotic stress. Plant, Cell & Environment 35(2): 259-270.
Tiwari BS, Belenghi B and Levine A, 2002. Oxidative stress increased respiration and generation of reactive oxygen species, resulting in ATP depletion, opening of mitochondrial permeability transition, and programmed cell death. Plant Physiology 128(2): 1271-1281.
Vensel WH, Tanaka CK, Cai N, Wong JH, Buchanan BB and Hurkman WJ, 2005. Developmental changes in the metabolic protein profiles of wheat endosperm. Proteomics 5(6): 1594-1611.
Wang WX, Vinocur B and Altman A, 2003. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218: 1-14.
Wei K, Jin X, Chen X, Wu F, Zhou W, Qiu B, Qiu L, Wang X, Li C and Zhang G, 2009. The effect of H2O2 and abscisic acid (ABA) interaction on β-amylase activity under osmotic stress during grain development in barley. Plant Physiology and Biochemistry 47(9): 778-784.
Ye W, Hu S, Wu L,& Changwei G, Cui Y, Wang X, Xu J, Ren D, Dong G, Qian Q and Guo L. 2016. White stripe leaf 12 (WSL12), encoding a nucleoside diphosphate kinase 2 (OsNDPK2), regulates chloroplast development and abiotic stress response in rice (Oryza sativa L.). Molecular Breeding 36: 57. 10.1007/s11032-016-0479-6.
Zhou J, Ma C, Zhen S, Cao M, Zeller FJ, Hsam SLK and Yan Y, 2016. Identification of drought stress related proteins from 1Sl (1B) chromosome substitution line of wheat variety Chinese Spring. Botanical Studies 57: 20. doi: 10.1186/s40529-016-0134-x.
Zor T and Selinger Z, 1996. Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. Analytical biochemistry 236(2): 302-308.
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