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Effect of plant growth regulators on Fittonia verschaffeltii regeneration at in vitro conditions | ||
| Journal of Plant Physiology and Breeding | ||
| مقاله 6، دوره 8، شماره 2، اسفند 2018، صفحه 59-68 اصل مقاله (737.42 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2018.9742 | ||
| نویسندگان | ||
| Mahsa Rashidi1؛ Ali Saremi-Rad* 2؛ Peyman Froozesh3؛ Vali Ollah Ghasemo Omran4 | ||
| 1Department of Agricultural Biotechnology, Faculty of Agriculture, Islamic Azad University, Damghan Branch, Damghan, Iran. | ||
| 2PhD student of Plant Breeding, Department of Agronomy and Plant Breeding, Young Researchers and Elite Club, Karaj Branch, Islamic Azad University, Karaj, Iran. | ||
| 3Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, Islamic Azad University, Karaj Branch, Karaj, Iran. | ||
| 4Genetic and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resource University, Sari, Iran. | ||
| چکیده | ||
| We investigated in vitro regeneration ability of Fittonia verschaffeltii using different explants (leaf, node) and plant growth regulators ((2,4-D, indoleacetic acid, indolebutyric acid (IBA), 6-benzylaminopurine (BAP), thidiazuron)). Percentage of callus induction, number of shoots, shoot length, percentage of rooting and root length were measured. The leaf explant showed higher frequency of callus induction in comparison with the node explant, while node had higher frequency of shoot formation. There were significant differences among plant growth regulators in terms of percentage of callus induction, number of shoots, percentage of rooting and root length, however, the difference was dependent on the type of explant. The highest percentage of callus induction (86.12%) was obtained for the leaf explant at Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-D and 1 mg/L IBA. The highest number of shoots (5.9 per explant) and shoot length (7.95 cm) were observed for the node explant at MS medium supplemented with 2 mg/L BAP + 1 mg/L IBA and MS medium containing 1mg/L BAP + 1 mg/L IBA, respectively. The highest percentage of root formation (87%) and root length (10.1 cm) were obtained at MS medium supplemented with 0.2 mg/L IBA and MS medium with no hormones, respectively. | ||
| کلیدواژهها | ||
| Callus induction؛ Explant؛ Fittonia verschaffeltii؛ In vitro culture؛ Plant growth regulator | ||
| مراجع | ||
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Akin-Idowu PE, Ibitoye DO and Ademoyegun OT, 2009. Tissue culture as a plant production technique for horticultural crops. African Journal of Biotechnology 8(16): 3782-3788. Akwatulira F, Gwali S, Okullo JBL, Ssegawa P, Tumwebaze SB, Mbwambo JR and Muchugi A, 2011. Influence of rooting media and indole-3-butyric acid (IBA) concentration on rooting and shoot formation of Warburgia ugandensis stem cuttings. African Journal of Plant Science 5(8): 421-429. Bercu R and Popoviciu D, 2015. Anatomy of Fittonia verschaffeltii (Lem.) Van Houtte (Acanthaceae). Annals of the Romanian Society for Cell Biology, 19(2): 23-28. Chauhan H, Desai SA and Khurana P, 2007. Comparative analysis of the differential regeneration response of various genotypes of Triticum aestivum, Triticum durum and Triticum dicoccum. Plant Cell, Tissue and Organ Culture 91(3): 191-199. Cheng M, Lowe BA, Spencer TM, Ye X and Armstrong CL, 2004. Factors influencing Agrobacterium-mediated transformation of monocotyledonous species. In Vitro Cellular and Developmental Biology-Plant 40(1): 31-45. Dhar U and Joshi M, 2005. Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew.(Asteraceae): effect of explant type, age and plant growth regulators. Plant Cell Reports 24(4): 195-200. Farjaminezhad R, Zare N, Asghari-Zakaria R and Farjaminezhad M, 2013. Establishment and optimization of cell growth in suspension culture of Papaver bracteatum: a biotechnology approach for thebaine production. Turkish Journal of Biology 37(6): 689-697. Jha P, Yadav C, Anjaiah V and Bhat V, 2009. In vitro plant regeneration through somatic embryogenesis and direct shoot organogenesis in Pennisetum glaucum (L.) R. Br. In Vitro Cellular & Developmental Biology-Plant 45(2): 145-154. Krasensky J and Jonak C, 2012. Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks. Journal of Experimental Botany 63(4): 1593-1608. Khawar K, Sarhin E, Sevimay C, Cocu S, Parmaksiz I, Uranbey S, Ipek A, Kaya M, Sancak C and Ozcan S, 2005. Adventitious shoot regeneration and micropropagation of Plantago lanceolata L. Periodicum Biologorum 107(1): 113-116. Machakova I, Zazimalova E and George EF, 2008. Plant growth regulators I: introduction; auxins, their analogues and inhibitors. In: George EF, Hall MA and De Klerk GJ (eds.). Plant Propagation by Tissue Culture. Pp. 175-204. Springer. Mendoza MG and Kaeppler HF, 2002. Auxin and sugar effects on callus induction and plant regeneration frequencies from mature embryos of wheat (Triticum aestivum L.). In Vitro Cellular and Developmental Biology-Plant 38(1): 39-45. Murashige T and Skoog F, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15(3): 473-497. Mwangi M, Ogero K, Mburugu G, Ombori O and Ngugi M, 2012. In vitro micropropagation of cassava through low cost tissue culture. Asian Journal of Agricultural Sciences 4(3): 205-209. Neelakandan AK and Wang K, 2012. Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications. Plant Cell Reports 31(4): 597-620. Nas MN, Bolek Y and Sevgin N, 2010. The effects of explant and cytokinin type on regeneration of Prunus microcarpa. Scientia Horticulturae 126(2): 88-94. Parmar SS, Sainger M, Chaudhary D and Jaiwal PK, 2012. Plant regeneration from mature embryo of commercial Indian bread wheat (Triticum aestivum L.) cultivars. Physiology and Molecular Biology of Plants 18(2): 177-183. Rashid H, Ghani RA, Chaudhry Z, Naqvi SMS and Quraishi A, 2002. Effect of media, growth regulators and genotypes on callus induction and regeneration in wheat (Triticum aestivum). Biotechnology 1(1): 49-54. Ren JP, Wang XG and Jun Y, 2010. Dicamba and sugar effects on callus induction and plant regeneration from mature embryo culture of wheat. Agricultural Sciences in China 9(1): 31-37. Rout G, Mohapatra A and Jain SM, 2006. Tissue culture of ornamental pot plant: a critical review on present scenario and future prospects. Biotechnology Advances 24(6): 531-560. Thorpe T, 2012. History of plant tissue culture. Plant Cell Culture Protocols 887: 9-27. Trejgell A, Tretyn A and Nicos D, 1998. Attempt at regeneration of Pharbitis nil from fragments of vegetative organs. Acta Physiologiae Plantarum 20(2): 161-166. Yildiz M, Özcan S and Er C, 2002. The effect of different explant sources on adventitious shoot regeneration in flax (Linum usitatissimum L.). Turkish Journal of Biology 26(1): 37-40. | ||
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