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Effects of various nitrogen sources on synchronization of tomato somatic embryogenesis during induction and realization phases | ||
| Journal of Plant Physiology and Breeding | ||
| مقاله 5، دوره 8، شماره 2، اسفند 2018، صفحه 53-58 اصل مقاله (537.22 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2018.9741 | ||
| نویسندگان | ||
| Aida Shomali1؛ Kambiz Mashayekhi* 1؛ Mohammadhadi Pahlevani2؛ Seyyed Javad Mousavizadeh1 | ||
| 1Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. | ||
| 2Department of Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. | ||
| چکیده | ||
| Tissue culture through somatic embryogenesis is one of the methods found most useful in the plants’ breeding process. A key issue to deal with during somatic evolution is its synchronization. Nitrogen has been known to play an important role here. Therefore, we evaluated the tomato explant embryogenesis cultured on B5 basal medium, subject to oxidized, reduced and organic nitrogen. Two separate experiments were conducted consisting of eight treatments with four replications each, using completely randomized design. In the experiment in which treatments were applied during the induction phase, maximum synchrony, based on relative number of torpedo embryo to all formed embryos, was obtained using nitrate as the sole nitrogen source (0.81). However, in the case in which treatments were applied during the realization phase, maximum synchrony was obtained through the combined nitrate and casein hydrolysate (0.51). Furthermore, in both experiments the highest number of somatic embryos was obtained in the standard B5 medium (91.58 in the first experiment and 59.19 in the second experiment). | ||
| کلیدواژهها | ||
| Ammonium؛ Casein hydrolysate؛ In vitro؛ Nitrate؛ Tomato | ||
| مراجع | ||
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Almagro A, Lin SH and Tsay YF, 2008. Characterization of the Arabidopsis nitrate transporter NRT1.6 reveals a role of nitrate in early embryo development. The Plant Cell 20(12): 3289-3299.
Al-Khayri JM, 2011. Influence of yeast extract and casein hydrolysate on callus multiplication and somatic embryogenesis of date palm (Phoenix dactylifera L.). Scientia Horticulturae 130(3): 531-535.
Bhojwani SS and Dantu PK, 2013. Plant Tissue Culture: An Introductory Text. Springer, India.
Böttcher C, Dennis EG, Booker GW, Polyak SW, Boss PK and Davies C, 2012. A novel tool for studying auxin-metabolism: the inhibition of grapevine indole-3-acetic acid-amido synthetases by a reaction intermediate analogue. PloS One 7(5): e37632. doi: 10.1371/journal.pone.0037632.
Cai Q, Szarejko I, Polok K and Maluszvnski M, 1992. The effect of sugars and growth regulators on embryoid formation and plant regeneration from barley anther culture. Plant Breeding, 109(3): 218-226.
Endres L, Souza BM and Mercier H, 2002. In vitro nitrogen nutrition and hormonal pattern in bromeliads. In Vitro Cellular and Developmental Biology-Plant 38(5): 481-486.
FAO, 2011. FAOSTAT Agriculture Data. http:// http://faostat.fao.org/site/567.
Fujimura T, 2014. Carrot somatic embryogenesis. A dream come true? Plant Biotechnology Reports 8(1): 23-28.
Gamborg OL, Miller RA and Ojima K, 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50(1): 151-158.
Kaur R and Kapoor M, 2016. Plant regeneration through somatic embryogenesis in sugarcane. Sugar Tech 18(1): 93-99.
Khierallah HS and Hussein NH, 2013. The role of coconut water and casein hydrolysate in somatic embryogenesis of date palm and genetic stability detection using RAP markers. Research in Biotechnology 4(3): 20-28.
Kim YW and Moon HK, 2007. Enhancement of somatic embryogenesis and plant regeneration in Japanese larch (Larix leptolepis). Plant Cell, Tissue and Organ Culture 88(3): 241-245.
Kumar S and Nadgauda R, 2014. Control of morphological aberrations in somatic embryogenesis of Commiphora wightii (Arnott) Bhandari (Family: Bursaraceae) through secondary somatic embryogenesis. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 85(1): 281-290.
Ludwig-Müller J, 2011. Auxin conjugates: their role for plant development and in the evolution of land plants. Journal of Experimental Botany 62(6): 1757-1773.
Mashayekhi-Nezamabadi K, 2000. The protein synthesis spectrum during the induction phase of somatic embryogenesis in carrot (Daucos carrota L.) cultures and the role of nitrogen forms for embryo development. A doctoral thesis. Justus Liebig University, Giessen, Germany.
Mihaljević S, Radić S, Bauer N, Garić R, Mihaljević B, Horvat G, Leljak-Levanić D and Jelaska S, 2011. Ammonium-related metabolic changes affect somatic embryogenesis in pumpkin (Cucurbita pepo L.). Journal of Plant Physiology 168(16): 1943-1951.
Murashige T and Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Physiologia Plantarum15: 473-497.
Pěnčík A, Turečková V, Paulišić S, Rolčík J, Strnad M and Mihaljević S, 2015. Ammonium regulates embryogenic potential in Cucurbita pepo through pH-mediated changes in endogenous auxin and abscisic acid. Plant Cell, Tissue and Organ Culture 122(1): 89-100.
Poothong S and Reed BM, 2014. Modeling the effects of mineral nutrition for improving growth and development of micropropagated red raspberries. Scientia Horticulturae 165: 132-141.
Poothong S and Reed BM, 2016. Optimizing shoot culture media for Rubus germplasm: the effects of NH4+, NO3− and total nitrogen. In Vitro Cellular and Developmental Biology-Plant 52(3): 265-275.
Trigiano RN and Gray DJ (eds.), 2004. Plant Development and Biotechnology. CRC Press, USA.
Zimmerman JL, 1993. Somatic embryogenesis: a model for early development in higher plants. The Plant Cell 5(10): 1411–1423. | ||
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