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بررسی مولکولی برخی تغییرات فیزیولوژیک در ژنوتیپ های حساس و متحمل ذرت در پاسخ به آلودگی ویروس موزاییک کوتولگی ذرت (Maize Dwarf Mosaic Virus; MDMV) | ||
| پژوهش های کاربردی در گیاهپزشکی | ||
| مقاله 1، دوره 7، شماره 3، پاییز 1397، صفحه 1-17 اصل مقاله (850 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| نویسندگان | ||
فروه سادات مصطفوی نیشابوری1؛ سیدکاظم صباغ  2؛ احمد یامچی3؛ سعید نصراله نژاد4؛ ناصر پنجه که5
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| 1دانشجوی دکتری بیماری شناسی گیاهی گروه گیاهپزشکی دانشگاه زابل. | ||
| 2دانشیار گروه زیست شناسی پردیس علوم دانشگاه یزد. | ||
| 3استادیار گروه اصلاح نباتات وبیوتکنولوژی دانشگاه علوم کشاورزی ومنابع طبیعی گرگان. | ||
| 4دانشیار گروه گیاهپزشکی دانشگاه علوم کشاورزی ومنابع طبیعی گرگان. | ||
| 5دانشیار گروه گیاهپزشکی دانشکده کشاورزی دانشگاه زابل. | ||
| چکیده | ||
| چکیده در این تحقیق تغییرات فعالیت آنزیمهای آنتیاکسیدانی کاتالاز و پراکسیداز به روش طیف سنجی و سطح بیان ژنهای NPR1، FKBP، Chlorophyll a-b binding protein و Metallothionein-like protein به روش واکنش زنجیرهای پلیمراز کمی (qRT-PCR) در دو ژنوتیپ متحمل (هیبرید 8) و حساس (SC705) ذرت در پاسخ به بیماری ویروس موزاییک کوتولوگی ذرت مورد ارزیابی قرار گرفت. آزمایش در شرایط گلخانه بصورت مایهزنی مکانیکی صورت گرفت و نمونهبرداری در زمانهای 0، 1، 9، 24 و 72 ساعت انجام شد. نتایج نشان دهندهی افزایش فعالیت پراکسیداز در ژنوتیپ متحمل در کلیهی زمانهای نمونهبرداری نسبت به ژنوتیپ حساس بود. بالاترین میزان افزایش در بازهی زمانی 24 ساعت بعد از مایهزنی درمقایسه با شاهد ثبت گردید. این میزان در بازهی زمانی 72 ساعت بعد از آلودهسازی نسبت به گیاهان کنترل کاهش یافت. فعالیت آنزیم کاتالاز در تمام بازههای زمانی بعد از آلودگی به میزان قابل ملاحظهای نسبت به گیاهان کنترل کمتر بود. تغییرات بیان ژنهای NPR1 و MT-LPدر هر دو ژنوتیپ افزیش داشته ولی این افزایش در گیاه متحمل بیشتر بود. میزان بیان ژن FKBPدر ژنوتیپ متحمل از اولین ساعت پس از آلودگی ویروسی افزایش یافت و با سرعت بیشتری نسبت به گیاه حساس کاهش یافت. تغییرات بیان در ژن Chlorophyll a-bدر گیاه حساس کاهش معنیداری نسبت به گیاهان شاهد داشت. تغییر فعالیت آنزیمهای آنتی اکسیدانی و میزان رونوشت ژنها در آلودگی ویروسی میتواند در مطالعات تعامل بین گیاه و ویروس به عنوان مارکر زیستی انتخابی برای مقاومت به بیماریهای ویروسی مورد استفاده قرار گیرد. | ||
| کلیدواژه‌ها | ||
| واژه های کلیدی: بیان ژن؛ پراکسیداز؛ کاتالاز؛ ویروس موزاییک کوتولگی ذرت | ||
| عنوان مقاله [English] | ||
| Molecular Study on Some of Physiological Changes in Susceptible and Tolerant Genotypes of Maize in Response to Maize Dwarf Mosaic Virus Infection | ||
| نویسندگان [English] | ||
| Farveh sadat Mostafavi Neishaburi1؛ seied kazem sabbagh2؛ ahmad yamchi3؛ saied nasrollahnejad4؛ naser Panjekeh5 | ||
| 1Ph. D. Student of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, University of Zabol, Iran. | ||
| 2Associate Professor, Department of Biology, Campus of Sciences. Yazd University, Iran. | ||
| 3Assistant Professor, Department of Plant Breeding & Biotechnology, Gorgon University of Agricultural Sciences & Natural Resources, Iran. | ||
| 4Associate Professor, Department of Plant Protection, Gorgon University of Agricultural Sciences & Natural Resources, Iran. | ||
| 5Associate Professor, Department of Plant Protection, Faculty of Agriculture, University of Zabol, Iran. | ||
| چکیده [English] | ||
| Abstract In this work, the changes of antioxidant enzymes activity were studied by spectrophotometry method and expression level of NPR1، FKBP، Chlorophyll a-b binding protein andMetallothionein-like protein genes in two tolerant and susceptible genotypes of maize in response to maize dwarf mosaic virus was investigated by qRT-PCR method. Experiment was done in green-house condition by mechanical inoculation and sampling was carried out at different time intervals (0, 1, 9, 24,72) hors post inoculation (hpi). The results showed a significant increase of peroxidase activity in the tolerant genotype at all sampling times when compared to the susceptible genotype. The highest increase of peroxidase enzyme was recorded at 24hpi for both genotypes in comparison to the control plants, but after 72hpi the rate of peroxidase enzyme was reduced. Catalase enzyme activity was significantly reduced at all sampling time after virus inoculation when compared to the control plants. Gene expression analysis showed that the related expression of NPR1 and MT-LP genes were increased in both genotypes but this increase was significant only in the tolerant genotype. The expression level of FKBP gene in the tolerant genotype increased at the early times after virus inoculation and was decreased more rapidly than susceptible cultivar. The expression changes of Chlorophyll a-b binding gene plants significantly reduced in susceptible plants compared to the control plants. Changes in the antioxidant enzyme activity and gene expression could be used as bio-markers for virus-plant interaction studies and the associated markers may be selected for plant resistance to viral disease | ||
| کلیدواژه‌ها [English] | ||
| Keywords: Catalase, Gene expression, Maize dwarf mosaic virus, Peroxidase | ||
| مراجع | ||
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