Introduction
Utilization of the laboratory animals in experimental biomedical researches and investigations have provided extended knowledge to better understanding the physiological, pathological, and immunological processes in human and animals (Clough, 1982). The results deriving from researches on these animals are affected by infectious diseases as well as housing conditions of these animals. Most of the zoonotic diseases may be transferred from the laboratory animals to humans. These include rat bite fever, tuberculosis, hemorrhagic fever, salmonellosis, lymphocytic choriomeningitis, leptospirosis, as well as various parasites such as Hymenolepis nana, H. diminuta, Syphacia muris, S. obvelata, Aspicularia tetraptera, Physaloptera spp., Taenia spp., Giardia spp., Trichomonas spp., Eimeria spp., Encephalitozoon cuniculi, Polyplax spp., lice, mites, etc.
Development of many biological assays depends on the usage of various laboratory animals. The most common laboratory animals used in research are rabbits, rats, mice, guinea pigs, and hamsters. It is indicated that the contaminated animals are not suitable for biomedical researches (Pam et al, 2013). Such a contaminations consist of parasitic, fungal, bacterial, and viral infections. Infectious diseases may affect research outcomes by altering pathophysiological, immunological, biochemical and hematological processes in hosts, increasing or decreasing host susceptibility to tissue damage, causing abnormal tissue growth, competing with the host for nutrients.
Among the nematodes infecting laboratory animals, the most common belong to the Oxyuridae Family. Rodent pinworms are mostly host-specific. Generally speaking, Syphacia obvelata and Aspicularia tetraptera are regarded as mouse pinworms, Syphacia muris, Syphacia mesocricetus and Dentostomella translucida regarded as rat, hamster and gerbil pinworms, respectively. S.obvelata has also been reported to infect humans (Kunstir et al., 1992; Pinto et al., 1994). The immunity to the infection is mostly homoral. Moreover, pinworms produce higher antibody production to non-parasitic antigenic stimuli (Kunstir et al., 1992; Pinto et al., 1994).
Giardia muris is a flagellated intestinal protozoan. Infections are occasionally detected in laboratory rodent colonies. Strains of G. muris infecting mice and rats may be host specific (Kunstir et al., 1992). The life cycle is direct. Environmentally resistant and infectious cysts are passed in the feces. Excystation occurs following ingestion. The minimum infectious dose for a mouse is approximately 10 cysts (Pinto et al., 1994). After the excystment, trophozoites divide longitudinally and colonize the mucosal surface of the proximal small intestine, adhering to columnar cells near the bases of intestinal villi moving within the mucus layer on the mucosa (National Research Council, 1991). Most infections are asymptomatic. When apparent, clinical signs are nonspecific and include weight loss, stunted growth, rough coat, and enlarged abdomen. In athymic or otherwise immunocompromised hosts, clinical signs may be more severe and may include diarrhea and death; and cyst shedding may be prolonged (Jungmann et al., 1996).
Spironucleus muris (formerly called Hexamita muris) is a second flagellated protozoan commonly infecting laboratory mice and rats. Host-specific strains of S. muris have been identified (Whitehouse et al., 1993). The minimum infective dose for a mouse is 1 cyst (Stachan et al., 1983). Infections with S. muris are asymptomatic in immunocompetent adult mice and rats. It has been reported by several investigators that young mice may develop diarrhea, dehydration, weight loss, rough coat, lethargy, abdominal distension, and hunched posture and may die (National Research Council, 1991; Whitehouse et al., 1993). In athymic (nu/nu) and lethally irradiated mice, S. muris causes severe chronic enteritis and weight loss. The crypts are hyperplastic and may be distended with trophozoites, microvilli and villi may be shortened, and enterocyte turnover is increased; inflammation is minimal (National Research Council, 1991; Whitehouse et al., 1993).
Pinworms commonly infecting laboratory rodents included the rat pinworm Syphacia muris and murine Syphacia obvelata and Aspicularis tetraptera. S. obvelata have also been reported to infect humans (National Research Council, 1991). The prevalence of infection remains high (National Research Council 1991; Jungmann et al., 1996), even in well-managed animal colonies. While infections are usually subclinical, rectal prolapse, intussusception, fecal impaction, poor weight gain and rough coat have been reported in heavily infected rodents, although generally without adequate exclusion of other pathogens (National Research Council, 1991). Athymic (nu/nu) mice are reportedly more susceptible to infection. There are few reports documenting the effects of pinworms on research. Pinworm infection resulted in significantly higher antibody production to sheep erythrocytes (Whitehouse et al., 1993), reduced the occurrence of adjuvant-induced arthritis and impaired intestinal electrolyte transport.
The aim of this study was to investigate the parasitic infections of laboratory animals maintained conventionally in animal houses of Tabriz Universities Research Centers.
Materials and Methods
This study was conducted on ecto- and endo-parasites of rats and mice in breeding and research conventional system animal houses of Universities of Tabriz. These centers include Medicine Faculty, Pharmacy Faculty, Drug Applied Research Center (DARC) of Tabriz University of Medical Sciences, Faculty of Veterinary Medicine, Khalatpoushan center at University of Tabriz, Azad University Tabriz Branch and finally Azad University Marand Branch. Seventy samples (35 rats and 35 mice) were selected randomly. Faecal samples were collected for parasitological examination (direct and staining). Two staining techniques were used: Trichrome staining for protozoa (Romia et al., 1990) and Modified Ziel-Nelsen staining for Cryptosporidium (Fayer et al., 2001). Blood samples were collected and stained with Giemsa staining (Shahbazi et al., 2011). Finally, cutaneous samples were collected in 70% Ethilic Alchohol. The results of the study were analyzed by excel software.
Results
All blood samples (100%) were negative for blood parasites. Fecal examinations revealed the cyst of Giardia muris (57%), Ascaris spp. eggs (17%), Oxyuris muris (93%), Syphacia muris (4%), Aspicularis tetraptera (2%), and Hymenolepis nana (9%). In cutaneous examinations, Polyplax serrata (21%) and lice nit (55%) were observed. Figures 1 and 2 show the percentages of various parasites of rats and mice isolated from the so-called centers. According to fig. 1, among 35 examined mice from all centers (DARC, Medicine Faculty, Pharmacy Faculty, Faculty of Veterinary Medicine (University of Tabriz), Khalatpoushan Center, Faculty of Veterinary Medicine (Azad University, Tabriz Branch), and Azad University Marand Branch) parasitic species consisted of Giardia (trophozoite and cyst) (88.5%), Ascaris egg (14.28%), Oxyuris egg (5.71%), Hymenolepis nana egg (22.85%), Syphacia obvelata egg (8.57%), Aspicularia tetraptera egg (5.71%), and lice nit (71.42%). The prevalence of various parasites isolated from rats is summarized in fig. 2.
Accordingly, the common parasites are: Giardia (trophozoite and cyst) 74.28%, Ascaris egg 28.57%, Oxyuris egg 2.85%, Syphacia obvelata 2.85%, Polyplax serrata 60%, and Nit 85.71%.
Fig. 1. Infection rate of the parasites isolated from mice: DARC (Drug & Applied Research Center of Tabriz University of Medical Sciences). Medicine Faculty, Pharmacology Faculty, Faculty of Veterinary Medicine University of Tabriz, Khalatpoushan Center, Faculty of Veterinary Medicine (Azad University Tabriz Branch) and Azad University Marand Branch.
Fig. 2. Infection rate of the parasites isolated from rats: DARC (Drug & Applied Research Center of Tabriz University of Medical Sciences). Medicine Faculty, Pharmacology Faculty, Faculty of Veterinary Medicine (University of Tabriz), Khalatpoushan Center, Faculty of Veterinary Medicine (Azad University, Tabriz Branch) and Azad University Marand Branch.
The fig. 3 to 10, show the parasites isolated from mice and rats.
Fig 3. Lice nit Fig 4. Hymenolepis nana egg
Fig 5. Syphacia obvelata egg Fig 6. Aspicularia egg
Fig 7. Ascaris spp. egg Fig 8. Giardia cyst
1؛ Ahmad Nematollahi2؛ Parisa Shahbazi2؛ Mehran Mesghari3
