آمار
| تعداد نشریات | 45 |
| تعداد شمارهها | 1,370 |
| تعداد مقالات | 16,844 |
| تعداد مشاهده مقاله | 54,231,491 |
| تعداد دریافت فایل اصل مقاله | 16,943,292 |
Antioxidant Isoenzymes Activities in Seedling Roots of Wheat Exposed to Drought Stress | ||
| Journal of Plant Physiology and Breeding | ||
| مقاله 4، دوره 3، شماره 2، اسفند 2013، صفحه 33-40 اصل مقاله (132.06 K) | ||
| نوع مقاله: Research Paper | ||
| چکیده | ||
| Abstract This study was conducted to evaluate the role of oxidative stress in roots of eight wheat genotypes with different drought tolerance, subjected to drought stress. For analyzing the changes of antioxidant enzymes, native PAGE analyses of protein extract were performed. The roots of wheat seedlings showed one unambiguous isoform of superoxide dismutase (SOD) and catalase (CAT). Eight isoforms of peroxidase (POX) were identified in the roots of wheat. The activities of SOD and POX isoforms decreased and the total activities of POX and CAT remained unchanged under the stress condition. The response of enzyme isoforms to drought were not the same for all isoforms of the antioxidant enzymes in the wheat genotypes, as POX isoforms showed the significant changes in the different drought tolerant genotypes. Significant interaction was observed between wheat group and stress treatments for total POX activity. POX total activity in the sensitive group of wheat genotypes was significantly higher than the tolerant group in the stress condition. | ||
| کلیدواژهها | ||
| Keywords: CAT؛ Drought Stress؛ Isozyme؛ POX؛ SOD؛ Wheat | ||
| مراجع | ||
|
Abogadallah GM and Serag MM, 2010. Fine and coarse regulation of reactive oxygen species in the salt tolerant mutants of barnyard grass and their wild type parents under salt stress. Physiol Plant 138: 60–73.
Alscher RG, Erturk N and Heath LS, 2002. Role of superoxide dismutases (SODs) in controlling oxidative stress. Exp Bot 53: 1331–1341.
Anderson MD, Prasad TK and Stewart CR, 1995. Changes in isozyme profiles of catalase, peroxidase and glutathione reductase during acclimation to chilling in mesocotyls of maize seedling. Plant Physiol 109: 1247-1257.
Bartoli CG, Simontacchi M, Tamambussi E and Beltrano J, 1999. Drought and watering-dependent oxidative stress: effect on antioxidant content in Triticum aestivum L. leaves. Exp Bot 50: 375-383.
Bowler C, Van Montagu M and Inz D, 1992. Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol 43: 83-116.
Cruz de Carvalho MH, 2008. Drought stress and reactive oxygen species: production, scavenging and signaling. Rev Plant Signaling & Behavior 3: 156-165.
Csiszár J, Pintér B, Kolbert Z, Erdei L and Tari I, 2008. Peroxidase activities in root segments of wheat genotypes under osmotic stress. Acta Biol Szeged 52: 155-156.
Davies WJ and Zhang J, 1991. Root signals and the regulation of growth and development of plants in drying soil. Annu Rev Plant Physiol Plant Mol Biol 42: 55–76.
Fu J and Huang B, 2001. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ Exp Bot 45: 105‑14.
Ghassemi-Golezani K, Khomari S and Valizadeh M, 2009. Effects of seed and seedling vigor on antioxidative isozyme activity and cold acclimation capability of winter oilseed rape. J Food Agr Environ 7: 452–456.
Guo Z, Ou W, Lu S and Zhong Q, 2006. Differential responses of antioxidative system to chilling and drought in four rice genotypes differing in sensitivity. Plant Physiol Biochem 44: 828–836.
Jiang M and Zhang J, 2002. Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and upregulates the activities of antioxidant enzymes in maize leaves. Exp Bot 53: 2401-2410.
Luna M, Badiani M, Felice M, Artemi F and Germanni G, 1985. Selective enzyme inactivation under water stress in maize (Zea mays L.) and wheat (Triticum aestivum L.) seedlings. Environ Exp Bot 25: 153-156.
Mittler R, 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7: 405-410.
Mittler R and Zilinskas BA, 1994. Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought. Plant J 5: 397-405.
Moran JF, Becana M, Iturbe-Ormaetxe I, Frechilla S, Klucas RV and Aparicio-Tejo P, 1994. Drought induces oxidative stress in pea plants. Planta 194: 346-352.
Noctor G and Foyer CH, 1998. Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49: 249-279.
Quartacci MF and Navaro LF, 1992. Water stress a free radical mediated changes in sunflower seedlings. Plant Physiol 142: 621-625.
Selote DS and Khanna-Chopra R, 2006. Drought-acclimation confers oxidative stress tolerance by inducing coordinated antioxidant defense at cellular and subcellular level in leaves of wheat seedlings. Physiol Plant 127: 494–506.
Soltis DE and Soltis PS, 1990. Isozymes in Plant Biology. Chpman and Hall, London, 259 pp.
Tsang EWT, Bowler C, Herouart D, Van Camp W, Villarroel R, Genetello C, Van MM and Inze D, 1991. Differential regulation of superoxide dismutase in plants exposed to environmental stress. Plant Cell 3: 783–792.
Turkan I, Bor M, Ozdemir F and Koca H, 2005. Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci 168: 223-231.
Van Rensburg L and Kru¨ger GHJ, 1994. Evaluation of components of oxidative stress metabolism for use in selection of drought tolerant genotypes of Nicotiana tabacum L. Plant Physiol 143: 730-737.
Zhang J and Kirkham MB, 1995. Water relations of water stressed, split-root C4 (Sorghum bicolor; Poaceae) and C3 (Helianthus annuus; Asteraceae) plants. Am J Bot 82: 1220-1229.
| ||
|
آمار تعداد مشاهده مقاله: 2,414 تعداد دریافت فایل اصل مقاله: 2,090 |
||