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Genetic diversity among Iranian Citrullus colocynthis accessions using morphological and phytochemical characteristics | ||
| Journal of Plant Physiology and Breeding | ||
| دوره 16، شماره 2، 2026، صفحه 87-104 اصل مقاله (1.35 M) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2026.21803 | ||
| نویسندگان | ||
| Maedeh Fereshtian* 1؛ Forozandeh Soltani* 2 | ||
| 1Department of Botany, Research Institute of Forests and Rangelands, Tehran, Iran. | ||
| 2Department of Horticultural Science and Landscape Architecture, Faculty of Agriculture & Natural Resources, University of Tehran, Karaj, Iran. | ||
| چکیده | ||
| Objective: Colocynth (Citrullus colocynthis L. Schrad.) is an important medicinal and oilseed species adapted to arid and semi-arid environments. This study aimed to characterize the morphological and phytochemical diversity among Iranian C. colocynthis accessions and identify superior genotypes with desirable yield and nutritional traits. Methods: Seventeen accessions, collected from different geographical regions of Iran, were evaluated under field conditions using a randomized complete block design with three replications. Morphological traits related to fruit and seed characteristics, along with phytochemical attributes- including fatty acid composition, total phenolic content, and carotenoid concentrations- were analyzed using several statistical methods, including analysis of variance, principal component analysis, and hierarchical clustering. Correlation coefficients among the morphological and phytochemical characteristics were also computed. Results: Significant variation was observed among the colocynth accessions for all of the evaluated traits, indicating substantial genetic diversity within the Iranian colocynth germplasm. The Jiroft, Khorasgan 5, Arak, and Orzoeeh accessions exhibited superior performance for fruit yield, and fruit number. Seed oil analysis revealed the predominance of unsaturated fatty acids, particularly linoleic acid, with the highest concentration detected in the Orzoeeh accession (73%), followed by Arak (72%), Hormozgan (72%), Jiroft (71%), and Khorasgan 5 (71%). Higher total phenolic content was observed in the Kerman and Yazd accessions, while the Ahvaz, Jiroft, and Arak accessions exhibited higher total carotenoid levels, suggesting a higher antioxidant potential and adaptation to environmental stress conditions. Principal component and cluster analyses confirmed the divergence among the colocynth accessions and differentiated them based on combined agronomic and phytochemical characteristics. Conclusion: This study represents one of the first evaluations integrating the morphological and phytochemical characteristics of Iranian colocynth germplasm. The results demonstrate substantial genetic diversity within Iranian colocynth germplasm for morphological and phytochemical characteristics. The high proportion of unsaturated fatty acids, particularly linoleic acid, further emphasizes the nutritional and pharmaceutical value of this germplasm. The identified superior accessions may serve as valuable genetic resources for breeding programs targeting drought tolerance, medicinal quality, antioxidant capacity, and seed oil improvement under arid climatic conditions. | ||
| کلیدواژهها | ||
| Antioxidant compounds؛ Citrullus colocynthis؛ Germplasm؛ Linoleic acid؛ Medicinal plant؛ Phytochemical diversity | ||
| مراجع | ||
|
Achigan-Dako EG, Fuchs G, Ahanchede A, Blattner FR. 2021. Flow cytometric analysis in Lagenaria siceraria (Cucurbitaceae) indicates correlation of genome size with usage types and growing elevation. Plant Syst Evol. 276: 9-19. http://doi:10.1007/s00606-008-0075-2
Akobundu ENT, Cherry JP, Simmons JG. 1982. Chemical, functional, and nutritional properties of Egusi (Colocynthis citrullus L.) seed protein products. J Food Sci. 47(3): 829-835. https://doi 10.1111/j.1365-2621.1982.tb12725.x
Alfawaz MA. 2004. Chemical composition and oil characteristics of pumpkin (Cucurbita maxima) seed kernels. Research Bultin No. 129, Food Science and Agriculture Research Center, King Saud University, Saudi Arabia.
Ashraf M, Akram NA, Al-Qurainy F, Foolad MR. 2011. Drought tolerance: role of organic osmolytes, growth regulators, and mineral nutrients. In: Sparks DL (ed.) Adv Agron. 111(76): 249-296. https://doi.org/10.1016/B978-0-12-387689-8.00002-3
Balkaya A, Özbakir M, Kurtar ES. 2010. The phenotypic diversity and fruit character-ization of winter squash (Cucurbita maxima) populations from the Black Sea region of Turkey. Afr J Biotechnol. 9(2): 152-162. https://doi.org/10.5897/AJB09.1416
Benariba N, Djaziri R, Bellakhdar W, Belkacem N, Kadiata M, Malaisse WJ. Sener A. 2013. Phytochemical screening and free radical scavenging activity of Citrullus colocynthis seeds extracts. Asian Pac J Trop Biomed. 3(1): 35-40. https://doi.org/10.1016/S2221-1691(13)60020-9
Blanco-Díaz MT, Del Río-Celestino M, Martínez-Valdivieso D, Font R. 2014. Use of visible and near-infrared spectroscopy for predicting antioxidant compounds in summer squash (Cucurbita pepo ssp pepo). Food Chem. 164: 301-308. https://doi.org/10.1016/j.foodchem.2014.05.019
Bourhia M, Bouothmany K, Bakrim H, Hadrach S, Salamatullah AM, Alzahrani A, Khalil Alyahya H, Albadr NA, Gmouh S, Laglaoui A, et al. 2021. Chemical profiling, antioxidant, antiproliferative, and antibacterial potentials of chemically characterized extract of Citrullus colocynthis L. seeds. Separations. 8(8): 114. http://doi.org/10.3390/separations8080114
Chawgien K, Kiattisin S. 2021. Machine learning techniques for classifying the sweetness of watermelon using acoustic signal and image processing. Comput Electron Agric. 181: 105938. http://doi.org/10.1016/j.compag.2020.105938
Elnaggar A, Tsombou FM, Hussain MI, Almehdi AM, Abideen Z, Hong Yong JW, and El-Keblawy A. 2024. Citrullus colocythis regulates photosynthetic and biochemical processes to develop stress resilience and sustain growth under sub-optimal temperatures. Plant Stress. 12: 100502. https://doi.org/10.1016/j.stress.2024.100502
Esquinas-Alcazar JT, Gulick PJ. 1983. Genetic resources of Cucurbitaceae: a global report. IBPGR, Rome, Italy.
Fathiazad F, Delazar A, Amiri R, Sarker SD. 2010. Extraction of flavonoids and quantification of rutin from waste tobacco leaves. Iran J Pharm Res. 5(3): 222-227. https://doi.org/10.22037/ijpr.2010.680
Ferriol MB, Pico J. 2008. Pumpkin and winter squash. In: Prohens J, Nuez F (eds). Vegetables I. Handbook of plant breeding, Vol 1. New York: Springer, pp. 317–349. https://doi.org/10.1007/978-0-387-30443-4-10
Folch J, Lees M, Sloane Stanley GH. 1957. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 226(1): 497-509.
Hussain AI, Rathore HA, Sattar MZA, Chatha SAS, Ahmad FUD, Ahmad A, Johns EJ. 2013. Phenolic profile and antioxidant activity of various extracts from Citrullus colocynthis (L.) from the Pakistani flora. Ind Crops Prod. 45: 416-422. https://doi.org/10.1016/j.indcrop.2013.01.002
Hussain AI, Rathore HA, Sattar MZ, Chatha SA, Sarker SD, Gilani AH. 2014. Citrullus colocynthis (L.) Schrad (bitter apple fruit): a review of its phytochemistry, pharmacology, traditional uses and nutritional potential. J Ethnopharmacol. 155(1): 54-66. https://doi.org/10.1016/j.jep.2014.06.011
Joseph JD, Ackman RG. 1992. Capillary column gas chromatographic method for analysis of encapsulated fish oils and fish oil ethyl esters: collaborative study. J AOAC Int. 75(3): 488-506. https://doi.org 10.1093/JAOAC/75.3.488
Lazos ES. 1986. Nutritional, fatty acid, and oil characteristics of pumpkin and melon seeds. J Food Sci. 51(5): 1382-1383. https://doi.org/10.1111/j.1365-2621.1986.tb13133.x
Maran JP, Mekala V, Manikandan S. 2013. Modeling and optimization of ultrasound-assisted extraction of polysaccharide from Cucurbita moschata. Carbohydr Polym. 92(2): 2018-2026. https://doi.org/10.1016/j.carbpol.2012.11.086
Marinova D, Ribarova F, Atanassova M. 2005. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J Univ Chem Technol Metall. 40(3): 255-260. https://www.researchgate.net/publication/258769164
Marzouk Z, Marzouk B, Mahjoub MA, Haloui E, Mighri Z, Aouni M, Fenina N. 2010. Screening of the antioxidant and the free radical scavenging potential of Tunisian Citrullus colocynthis Schrad. from Mednine. J Food Agric Environ. 8(2): 261-265.
Mohammadi SA, Prasanna BM. 2003. Analysis of genetic diversity in crop plants- salient statistical tools and considerations. Crop Sci. 43(4): 1235–1248. https://doi.org/10.2135/cropsci2003.1235
Montes-Hernández S, Merrick L, Eguiarte L. 2005. Maintenance of squash (Cucurbita spp.) landrace diversity by farmer’s activities in Mexico. Genet Resour Crop Evol. 52(22): 697-707. https://doi.org /10.1007/s10722-003-6018-4
Morovati Z, Karimzadeh G, Rashidi Monfared S, Naghavi MR. 2024. Evaluation of morphophysiological and phytochemical characteristics of Datura stramonium and D. innoxia from Iran under controlled conditions. J Plant Physiol Breed. 14(2): 39-57. http//doi.org/10.22034/jppb.2024.61219.1334
Nayab D, Ali D, Arshad N, Malik A, Choudhary MI, Ahmed Z. 2006. Cucurbitacin glucosides from Citrullus colocynthis. Nat Prod Res. 20(5): 409-413. https://doi.org/10.1080/14786410500044997
Shahid M, Rao NK. 2014. Diversity of Citrullus colocynthis (L.) Schrad. (Cucurbitaceae) in the United Arab Emirates. J New Biol Rep. 3(2): 145-150.
Sneath PHA, Sokal RP. 1973. Numerical taxonomy: the principles and practice of numerical classification. First Edition. San Francisco: W.H. Freeman, 573 pp.
Tannin-Spitz T, Bergman M, Grossman S. 2007. Cucurbitacin glucosides: antioxidant and free-radical scavenging activities. Biochem Biophys Res Commun. 364(1): 181-186. https://doi.org/10.1016/j.bbrc.2007.09.075
Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH. 2006. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compost Anal. 19(6-7): 669-675. https://doi.org/10.1016/j.jfca.2006.01.003
Thamer FH, Thamer N. 2023. Gas Chromatography-Mass spectrometry (GC-MS) profiling reveals newly described bioactive compound in Citrullus colocynthis (L). seeds oil extracts. Heliyon. 6(9): 16861. https://doi.org/10.1016/j.heliyon.2023.e16861
Ziyada AK, Elhussien SA. 2008. Physical and chemical characteristics of Citrullus lanatus var. Colocynthoide seed oil. J Phys Sci. 19(2): 69-75.
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