Interactive effects of irrigation levels, fulvic acid, and shading intensity on the growth, physiological, and biochemical traits of soilless-grown rocket (Eruca sativa Mill.)

Ahmed Mohammed Amin, Mohammed; Sarabi, Behrooz; Mozfari, Ali Akbar

doi:10.22034/jppb.2025.68975.1378

فهرست نشریات دارای اعتبار وزارت علوم، تحقیقات و فناوری

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	Interactive effects of irrigation levels, fulvic acid, and shading intensity on the growth, physiological, and biochemical traits of soilless-grown rocket (Eruca sativa Mill.)
Journal of Plant Physiology and Breeding
دوره 15، شماره 2، 2025، صفحه 217-242 اصل مقاله (683.42 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22034/jppb.2025.68975.1378
نویسندگان
Mohammed Ahmed Mohammed Amin¹؛ Behrooz Sarabi^* ²؛ Ali Akbar Mozfari²
¹Department of Horticultural Sciences and Engineering, Faculty of Agriculture, University of Kurdistan, P.O. Box 66177-15175, Sanandaj, Kurdistan, Iran; Protected Agriculture Department, Bakrajo Technical Institute, Sulaimani Polytechnic University, Sulaymaniyah, Kurdistan, Iraq.
²Department of Horticultural Sciences and Engineering, Faculty of Agriculture, University of Kurdistan, P.O. Box 66177-15175, Sanandaj, Kurdistan, Iran; Research Center of Medicinal Plants Breeding and Development, University of Kurdistan, Sanandaj, Kurdistan, Iran.
چکیده
Objective: This study aimed to assess the interactive effects of irrigation levels, foliar application of fulvic acid, and shading intensity on the growth, physiological performance, and biochemical traits of rocket (Eruca sativa Mill.), cultivated in soilless conditions under greenhouse environments. Methods: A factorial experiment was conducted, using a completely randomized design with three replications. The first factor consisted of three different irrigation levels, 100%, 75%, and 50% of the plants' water requirements, referred to as IL100, IL75, and IL50, respectively. The second and third factors included three concentrations of foliar fulvic acid (FA): 0, 1, and 2 g L⁻¹, along with three shading intensities of 0%, 50%, and 80%, respectively. Rocket plants were cultivated in a controlled hydroponic system, and growth-related traits, as well as physiological and biochemical characteristics, were measured. The data were analyzed using analysis of variance to determine the significance of the main effects and their interaction for the measured traits. Results: Most of the Main effects of the factors and their interactions were significant for most of the measured characteristics. The highest shoot and root biomasses were observed under full irrigation (IL100), without shading, and with 2 g L⁻¹ of FA, while severe shading (80%) and a water deficit (IL50) significantly diminished growth. Notably, IL50 resulted in increases of up to 27% in chlorophyll a, chlorophyll b, and carotenoid contents compared to IL100, with the application of FA enhancing this effect. Relative water content ranged from 86.36% to 87.78%, and the membrane stability index varied from 76.46% to 78.59%, both reaching their highest values under full irrigation, moderate shading, and high FA conditions. Malondialdehyde concentration, which indicates oxidative stress, was lowest under conditions of full irrigation, high shading, and the application of FA. Also, plants grown under IL50 without shading and treated with 2 g L⁻¹ of FA showed the highest concentrations of soluble carbohydrates and proline. These levels were approximately 200% and 73% higher, respectively, compared to control plants that were grown under IL100 with 80% shading and no FA. Conclusion: The optimized coordination of irrigation, foliar FA application, and shading intensity significantly enhances the growth, physiological, and biochemical tolerance of rocket plants. This strategy promotes sustainable and efficient soilless cultivation of leafy vegetables such as rocket in greenhouse environments.
کلیدواژه‌ها
Biostimulants؛ Controlled environment cultivation؛ Irrigation level؛ Light intensity

مراجع
Abhari A, Gholinezhad E. 2019. Effect of humic acid on grain yield and yield components in chickpea under different irrigation levels. J Plant Physiol Breed. 9: 19-29. https://doi.org/10.22034/jppb.2019.10441 Ahemd HA, Al-Faraj AA, Abdel-Ghany AM. 2016. Shading greenhouses to improve the microclimate, energy and water saving in hot regions: a review. Sci Hortic. 201: 36-45. https://doi.org/10.1016/j.scienta.2016.01.030 Ahmad Z, Waraich EA, Akhtar S, Anjum S, Ahmad T, Mahboob W, Hafeez OBA, Tapera T, Labuschagne M, Rizwan M. 2018. Physiological responses of wheat to drought stress and its mitigation approaches. Acta Physiol Plant. 40: 80. https://doi.org/10.1007/s11738-018-2651-6 Ali IJ, Jafari N, Sheikh Amiri A. 2024. Priming the seeds of two mung bean (Vigna radiata L.) varieties with ascorbic acid under drought stress. J Plant Physiol Breed. 14: 59-80. https://doi.org/10.22034/jppb.2024.64490.1351 Alsudays IM, Alshammary FH, Alabdallah NM, Alatawi A, Alotaibi MM, Alwutayd KM, Alharbi MM, Alghanem SMS, Alzuaibr FM, Gharib HS, et al. 2024. Applications of humic and fulvic acid under saline soil conditions to improve growth and yield in barley. BMC Plant Biol. 24: 191. https://doi.org/10.1186/s12870-024-04863-6 Anjum SA, Wang L, Farooq M, Xue L, Ali S. 2011. Fulvic acid application improves the maize performance under well‐watered and drought conditions. J Agron Crop Sci. 197: 409-417. https://doi.org/10.1111/j.1439-037x.2011.00483.x Arnon AN. 1967. Method of extraction of chlorophyll in the plants. Agron J. 23: 112-121. Arthurs SP, Stamps RH, Giglia FF. 2013. Environmental modification inside photoselective shadehouses. HortScience. 48: 975-979. https://doi.org/10.21273/hortsci.48.8.975 Babarabie M, Zarei H, Badeli S, Danyaei A. 2020. Humic acid and folic acid application improve marketable traits of cut tuberose (Polianthes tuberosa). J Plant Physiol Breed. 10: 85-91. https://doi.org/10.22034/JPPB.2020.12526 Bates LS, Waldren RP, Teare ID. 1973. Rapid determination of free proline for water-stress studies. Plant Soil. 39: 205-207. https://doi.org/10.1007/bf00018060 Batista DS, Felipe SHS, Silva TD, de Castro KM, Mamedes-Rodrigues TC, Miranda NA, Ríos-Ríos AM, Faria DV, Fortini EA, Chagas K, et al. 2018. Light quality in plant tissue culture: does it matter? Vitr Cell Dev Biol. 54: 195-215. https://doi.org/10.1007/s11627-018-9902-5 Bayat H, Shafie F, Aminifard MH, Daghighi S. 2021. Comparative effects of humic and fulvic acids as biostimulants on growth, antioxidant activity and nutrient content of yarrow (Achillea millefolium L.). Sci Hortic. 279: 109912. https://doi.org/10.1016/j.scienta.2021.109912 Bell L, Oruna-Concha MJ, Wagstaff C. 2015. Identification and quantification of glucosinolate and flavonol compounds in rocket salad (Eruca sativa, Eruca vesicaria and Diplotaxis tenuifolia) by LC–MS: highlighting the potential for improving nutritional value of rocket crops. Food Chem. 172: 852-861. https://doi.org/10.1016/j.foodchem.2014.09.116 Bulgari R, Franzoni G, Ferrante A. 2019. Biostimulants application in horticultural crops under abiotic stress conditions. Agronomy. 9: 306. https://doi.org/10.3390/agronomy9060306 Cameron RWF, Harrison-Murray RS, Scott MA. 1999. The use of controlled water stress to manipulate growth of container-grown Rhododendron cv. Hoppy. J Hortic Sci Biotechnol. 74: 161-69. https://doi.org/10.1080/14620316.1999.11511089 Canellas LP, Olivares FL, Aguiar NO, Jones DL, Nebbioso A, Mazzei P, Piccolo A. 2015. Humic and fulvic acids as biostimulants in horticulture. Sci Hortic. 196: 15-27. https://doi.org/10.1016/j.scienta.2015.09.013 Caruso G, Formisano L, Cozzolino E, Pannico A, El-Nakhel C, Rouphael Y, Tallarita A, Cenvinzo V, De Pascale S. 2020. Shading affects yield, elemental composition and antioxidants of perennial wall rocket crops grown from spring to summer in southern Italy. Plants. 9: 933. https://doi.org/10.3390/plants9080933 Chai Q, Gan Y, Zhao C, Xu H-L, Waskom RM, Niu Y, Siddique KHM. 2016. Regulated deficit irrigation for crop production under drought stress. A review. Agron Sustain Dev. 36: 3. https://doi.org/10.1007/s13593-015-0338-6 Chaves MM, Flexas J, Pinheiro C. 2009. Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann Bot. 103: 551-560. https://doi.org/10.1093/aob/mcn125 Du Jardin P. 2015. Plant biostimulants: definition, concept, main categories and regulation. Sci Hortic. 196: 3-14. https://doi.org/10.1016/j.scienta.2015.09.021 Flexas J, Barbour MM, Brendel O, Cabrera HM, Carriquí M, Díaz-Espejo A, Douthe C, Dreyer E, Ferrio JP, Gago J, et al. 2012. Mesophyll diffusion conductance to CO₂: an unappreciated central player in photosynthesis. Plant Sci. 193: 70-84. https://doi.org/10.1016/j.plantsci.2012.05.009 Garg G, Sharma V. 2014. Eruca sativa (L.): botanical description, crop improvement, and medicinal properties. J Herbs Spices Med Plants. 20: 171-182. https://doi.org/10.1080/10496475.2013.848254 Ghassemi Golezani K, Ardalan N, Raei Y, Dalil B. 2022. Improving some physiological and yield parameters of safflower by foliar sprays of Fe and Zn under drought stress. J Plant Physiol Breed. 12: 15-27. https://doi.org/10.22034/jppb.2022.14657 González-Espíndola LÁ, Pedroza-Sandoval A, Trejo-Calzada R, Jacobo-Salcedo MDR, García de Los Santos G, Quezada-Rivera JJ. 2024. Relative water content, chlorophyll index, and photosynthetic pigments on Lotus corniculatus L. in response to water deficit. Plants. 13: 961. https://doi.org/10.3390/plants13070961 Han K, Wang C, Gao Y, Zhang J, Xie J. 2025. Response of amino acids, phenolic acids, organic acids, and mineral elements to fulvic acid in spinach (Spinacia oleracea L.) under nitrate stress. Sci Rep. 15: 9444. https://doi.org/10.1038/s41598-025-93974-7 Heath RL, Packer L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys. 125: 189-198. https://doi.org/10.1016/j.abb.2022.109248 Heidari M, Golpayegani A. 2012. Effects of water stress and inoculation with plant growth promoting rhizobacteria (PGPR) on antioxidant status and photosynthetic pigments in basil (Ocimum basilicum L.). J Saudi Soc Agric Sci. 11: 57-61. https://doi.org/10.1016/j.jssas.2011.09.001 Hosseinifard M, Stefaniak S, Ghorbani Javid M, Soltani E, Wojtyla Ł, Garnczarska M. 2022. Contribution of exogenous proline to abiotic stresses tolerance in plants: a review. Int J Mol Sci. 23: 5186. https://doi.org/10.3390/ijms23095186 Hussain S, Iqbal N, Rahman T, Liu T, Brestic M, Safdar ME, Asghar MA, Farooq MU, Shafiq I, Ali A, et al. 2019. Shade effect on carbohydrates dynamics and stem strength of soybean genotypes. Environ Exp Bot. 162: 374-382. https://doi.org/10.1016/j.envexpbot.2019.03.011 Irigoyen JJ, Einerich DW, Sánchez‐Díaz M. 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativd) plants. Physiol Plant. 84: 55-60. https://doi.org/10.1111/j.1399-3054.1992.tb08764.x Keskin B, Akhoundnejad Y, Dasgan HY, Gruda NS. 2025. Fulvic acid, amino acids, and vermicompost enhanced yield and improved nutrient profile of soilless iceberg lettuce. Plants. 14: 609. https://doi.org/10.3390/plants14040609 Khan I, Sohail, Zaman S, Li G, Fu M. 2025. Adaptive responses of plants to light stress: mechanisms of photoprotection and acclimation. A review. Front Plant Sci. 16: 1550125. https://doi.org/10.3389/fpls.2025.1550125 Parkash V, Singh S. 2020. A review on potential plant-based water stress indicators for vegetable crops. Sustainability. 12: 3945. https://doi.org/10.3390/su12103945 Pereira LS, Oweis T, Zairi A. 2002. Irrigation management under water scarcity. Agric Water Manag. 57: 175-206. https://doi.org/10.1016/s0378-3774(02)00075-6 Raza MA, Feng LY, Iqbal N, Khan I, Meraj TA, Xi ZJ, Naeem M, Ahmed S, Sattar MT, Chen YK, et al. 2020. Effects of contrasting shade treatments on the carbon production and antioxidant activities of soybean plants. Funct Plant Biol. 47: 342-354. https://doi.org/10.1071/fp19213 Renzetti M, Funck D, Trovato M. 2024. Proline and ROS: a unified mechanism in plant development and stress response? Plants. 14: 2. https://doi.org/10.3390/plants14010002 Resh HM. 2022. Hydroponic food production: a definitive guidebook for the advanced home gardener and the commercial hydroponic grower. Boca Raton, Fla: CRC Press. https://doi.org/10.1201/9781003133254 Sairam RK, Rao KV, Srivastava GC. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci. 163: 1037-1046. https://doi.org/10.1016/s0168-9452(02)00278-9 Santander C, González F, Pérez U, Ruiz A, Aroca R, Santos C, Cornejo P, Vidal G. 2024. Enhancing water status and nutrient uptake in drought-stressed lettuce plants (Lactuca sativa l.) via inoculation with different Bacillus spp. isolated from the Atacama Desert. Plants. 13: 158. https://doi.org/10.3390/plants13020158 Sarabi B. 2024. Effect of nutrient solution concentrations and irrigation levels combined with humic acid on physiological and quality characteristics of rocket crop (Eruca sativa (Mill.) Thell.). Arid L Res Manag. 38: 201-225. https://doi.org/10.1080/15324982.2023.2284361 Sarabi B, Ghaderi N, Ghashghaie J. 2022. Light-emitting diode combined with humic acid improve the nutritional quality and enzyme activities of nitrate assimilation in rocket (Eruca sativa (Mill.) Thell.). Plant Physiol Biochem. 187: 11-24. https://doi.org/10.1016/j.plaphy.2022.07.035 Song H, Zhu W, Guo Z, Song T, Wang J, Gao C, Zhang H, Shen R. 2025. The impact of fulvic acid on the growth physiology, yield, and quality of tomatoes under drought conditions. Agronomy. 15: 1528. https://doi.org/10.3390/agronomy15071528 Souza AC, Olivares FL, Peres LEP, Piccolo A, Canellas LP. 2022. Plant hormone crosstalk mediated by humic acids. Chem Biol Technol Agric. 9: 29. https://doi.org/10.1186/s40538-022-00295-2 Stamps RH. 2009. Use of colored shade netting in horticulture. HortScience. 44: 239-241. https://doi.org/10.21273/hortsci.44.2.239 Touil S, Richa A, Fizir M, Bingwa B. 2021. Shading effect of photovoltaic panels on horticulture crops production: a mini review. Rev Environ Sci Biotechnol. 20: 281-296. https://doi.org/10.1007/s11157-021-09572-2 Vitale E, Velikova V, Tsonev T, Ferrandino I, Capriello T, Arena C. 2021. The interplay between light quality and biostimulant application affects the antioxidant capacity and photosynthetic traits of soybean (Glycine max L. Merrill). Plants. 10: 861. https://doi.org/10.3390/plants10050861 Wang Z, Yang Y, Yadav V, Zhao W, He Y, Zhang X, Wei C. 2022. Drought-induced proline is mainly synthesized in leaves and transported to roots in watermelon under water deficit. Hortic Plant J. 8: 615-626. https://doi.org/10.1016/j.hpj.2022.06.009 Wu Y, Qiu T, Shen Z, Wu Y, Lu D, He J. 2018. Effects of shading on leaf physiology and morphology in the ‘Yinhong’grape plants. Rev Bras Frutic. 40: e-037. https://doi.org/10.1590/0100-29452018037 Xiu WU, Jie GAO. 2014. Effects of low light on agronomic and physiological characteristics of rice including grain yield and quality. Rice Sci. 21: 243-251. https://doi.org/10.1016/s1672-6308(13)60192-4 Zhang X, Wang Z, Li Y, Guo R, Liu E, Liu X, Gu F, Yang Z, Li S, Zhong X, et al. 2022. Wheat genotypes with higher yield sensitivity to drought overproduced proline and lost minor biomass under severe water stress. Front Plant Sci. 13: 1035038. https://doi.org/10.3389/fpls.2022.1035038 Živanović B, Milić Komić S, Tosti T, Vidović M, Prokić L, Veljović Jovanović S. 2020. Leaf soluble sugars and free amino acids as important components of abscisic acid-mediated drought response in tomato. Plants. 9: 1147. https://doi.org/10.3390/plants9091147
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Interactive effects of irrigation levels, fulvic acid, and shading intensity on the growth, physiological, and biochemical traits of soilless-grown rocket (Eruca sativa Mill.)