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Micropropagation of Aglaonema ‘Pink Lady’ for authentic varietal production | ||
| Journal of Plant Physiology and Breeding | ||
| دوره 15، شماره 2، 2025، صفحه 1-16 اصل مقاله (756.24 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2025.66233.1363 | ||
| نویسندگان | ||
| Mostafa Khoshhal Sarmast* 1؛ Rezvane Sookhtesarayi2 | ||
| 1Departmemt of Horticultural Sciences, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources | ||
| 2Department of Horticultural Science and Landscape Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| چکیده | ||
| Objective: Aglaonema, belonging to the Araceae family, is an evergreen herbaceous plant with attractive ornamental foliage suitable for home decoration. Traditional propagation methods, such as stem cuttings and plant division, have low efficiency and high costs. Therefore, the aim of this study was to evaluate the feasibility of in vitro propagation of Aglaonema ‘Pink Lady’. Methods: Aglaonema ‘Pink Lady’ stem cuttings were disinfected with 70% ethanol for 30 seconds and immersed in 10% Clorox solution for 10-30 minutes at the Tissue Culture Laboratory, University of Agricultural Sciences and Natural Resources, Gorgan, Iran (2023-2024). The washed explants were cultured on the MS medium supplemented with different concentrations of 6-benzyladenine (BA) (0–1 mg/L) and naphthaleneacetic acid (NAA) (0–1.5 mg/L). Additionally, the effects of higher thidiazuron (TDZ) levels (2–16 mg/L) on direct shoot induction were evaluated. The experiment was arranged in a completely randomized design with four replications. Explants were placed in jars containing 30 mL of culture medium and incubated at 25±1°C with 16 hours of light and 8 hours of darkness. Data were collected after 40 and 80 days, measuring callus weight, number and length of shoots, and number and length of roots. Results: The highest number of Aglaonema shoots (two shoots) was obtained in the MS medium containing 1 mg/L BA and 0.5 mg/L NAA after 80 days of culture. The use of 1 mg/L TDZ produced the highest average shoot length, followed by 0.5 mg/L TDZ combined with 0.5 mg/L NAA. The maximum number of roots for the regenerated Aglaonema was observed at 0 and 0.25 mg/L BA combined with different levels of NAA (0.5, 1, and 1.5 mg/L) after 40 days of culture. The use of high levels of TDZ, such as concentrations of 9 and 16 mg/L, increased the average number of shoots per stem explant to 40. Rooted plantlets, when transferred to the greenhouse, achieved more than 90% acclimatization. All regenerated plants retained the morphological characteristics of the corresponding mother plant. Conclusion: This experiment demonstrated that high levels of TDZ can significantly lead to direct organogenesis in stem explants of the Aglaonema cultivar ‘Pink Lady’. However, the genetic variability of the regenerated shoots for producing plants similar to the original remains to be continuously monitored. | ||
| کلیدواژهها | ||
| Micropropagation؛ Ornamental plants؛ Shoot proliferation؛ Thidiazuron | ||
| مراجع | ||
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Anjarsari IRD, Hamdani JS, Suherman C, Nurmala T. 2019. Effect of pruning and cytokinin application on the growth of tea GMB 7 clone. Asian J Plant Sci. 18(3): 110-116. https://doi.org/10.3923/ajps.2019.110.116
Bakti C, Murgayanti A, Mubarok S. 2011. Aglaonema micropropagation by in vitro culture. Indonesian J Agric Res. 28(2): 121.
Barakat AA, Gaber MK. 2018. Micropropagation and ex vitro acclimatization of Aglaonema plants. Middle East J Appl Sci. 8(4): 1425-1436.
Chen WL, Yeh DM. 2007. Elimination of in vitro contamination, shoot multiplication, and ex vitro rooting of Aglaonema. HortScience. 42(3): 629-632. https://doi.org/10.21273/HORTSCI.42.3.629
El-Gedawey HI, Hussein SE. 2022. Micropropagation of Aglonema ‘Lady Valentine’ by axillary shoots explants. Egypt Acad J Biolog Sci, H Botany. 13(2): 129-142. https://doi.org/10.21608/eajbsh.2022.273593
Gammoudi N, Pedro TS, Ferchichi A, Gisbert C. 2018. Improvement of regeneration in pepper: a recalcitrant species. In Vitro Cell Dev Biol- Plant. 54: 145-153. https://doi.org/10.1007/s11627-017-9838-1
Hartmann HT, Kester DE, Davis Jr FT, Geneve RL. 1997. Plant propagation: principles and practices. 6th edition. New Jersey, USA: Prentice Hall, pp. 540-611.
Hussein MMM. 2002. In vitro propagation of three species of Aglaonema plants. Bull Fac Agric Cairo Univ. 53: 465-488.
Kaviani B, Sedaghathoor Sh, Safari Motlagh MR, Rouhi S. 2019. Influence of plant growth regulators (BA, TDZ, 2-iP and NAA) on micropropagation of Aglaonema widuri. Iranian J Plant Physiol. 9: 2901-2909. https://doi.org/10.30495/ijpp.2019.668856
Kazemzadeh Bahnamirei M, Sarmast MK, Padasht Dahkaei MN, Alizadeh M. 2025. Evaluation of mother-plant growing beds, explant type, and different disinfection treatments in control of Sansevieria rhizome contamination under in vitro conditions. J Plant Physiol Breed. 14(2): 23-28. https://doi.org/10.22034/JPPB.2024.63310.1346
Kumar N, Reddy MP. 2011. In vitro plant propagation: a review. J For Sci. 27(2): 61-72. https://doi.org/10.7747/JFS.2011.27.2.1
Mariani TS, Fitriani A, da Silva JAT, Wicaksono A, Chia TF. 2011. Micropropagation of Aglaonema using axillary shoot explants. Int J Basic Appl Sci. 11(1): 46-53.
Nazary Moghaddam Aghayeh R, Abedy B, Balandari A, Samiei L, Tehranifar A. 2019. Contamination control in Iranian seedless barberry micropropagation. J Plant Physiol Breed. 9(1): 97-110. https://doi.org/10.22034/jppb.2019.10387
Salami AR, Ebadi A, Zamani Z, Ghasemi M. 2005. Improvement in apex culture in an Iranian grapevine (Vitis vinifera L. ‘Bidaneh Sefid’) through fragmented shoot apices. Int J Agric Biol. 7(3): 333-336.
Sharma N, Kumar N, James J, Kalia S, Joshi S. 2023. Strategies for successful acclimatization and hardening of in vitro regenerated plants: challenges and innovations in micropropagation techniques. Plant Sci Today. 10: 90-97. https://doi.org/10.14719/pst.2376
Shijun Z, Rulan J, Hougao Z. 2004. Rapid propagation study on Aglaonema commutatum cv. ‘Golden Jewelry’. Chinese Agric Sci Bull. 20(4): 39. https://doi.org/10.11924/j.issn.1000-6850.040439
Taiz L, Zeiger E. 2010. Plant physiology. 5th edition. Sunderland, UK: Sinauer Associates, 782 p.
Zahara M, Win CC. 2020. A review: the effect of plant growth regulators on micropropagation of Aglaonema sp. J Trop Hortic. 3(2): 96-100. http://dx.doi.org/10.33089/jthort.v3i2.58 | ||
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