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Effect of auxins on stem cutting propagation of three pomegranate genotypes and the relationship of endogenous phenolic compounds with root induction | ||
| Journal of Plant Physiology and Breeding | ||
| مقاله 3، دوره 15، شماره 1، 2025، صفحه 37-52 اصل مقاله (592.91 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2025.65189.1355 | ||
| نویسندگان | ||
| Sara Ghafouri؛ Mahdi Alizadeh* ؛ Azim Ghasemnezhad | ||
| Department of Horticulture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. | ||
| چکیده | ||
| Objective: The commercial propagation of pomegranates often relies on hardwood stem cuttings taken during the dormant season, and a link has been observed between phenolic compounds in donor plants and the success of adventitious rooting. This study aimed to investigate the effects of seasons, auxin, and genetic variation on pomegranate propagation, as well as the relationship between endogenous phenolic compounds and root induction. Methods: In this study, the seasonal variation of phenolic compounds in the shoot skins of three pomegranate genotypes: wild (Punica granatum cv. spinosa) and two local cultivars (Malas Mumtaz and Shirin Behshahr) was measured. Samples were collected at the end of each season and analyzed for phenolic compounds (gallic acid, caffeic acid, and quercetin) using HPLC, alongside total phenols, flavonoids, and antioxidant activity through spectrophotometric methods. Then, in mid-February, hardwood cuttings procured from the same mother plants were taken, treated with auxin or left untreated, and planted in a sand substrate. Rooting traits were measured, and the relationship of phenolic compounds with rooting traits was assessed. Results: In all seasons, the highest concentration of flavonoids and caffeic acid belonged to Shirin Behshahr and Malas Mumtaz pomegranate cultivars, respectively. Also, wild pomegranate showed the highest antioxidant capacity (DPPH) on average of four seasons, followed by Shirin Behshahr. The shoots collected during the winter season mainly had lower levels of caffeic acid and the lowest DPPH, but the summer season had the highest antioxidant capacity. Considering the auxin experiment, under no auxin, the Malas Mumtaz variety exhibited significantly higher rooting percentage (93.33%) than Shirin Behshahr and the wild genotype (56.67% and 50%, respectively). Although the auxin treatment at 2000 mg/L had a positive effect on all genotypes (100%, 96.67%, and 86.67% rooting for the wild genotype, Malas Mumtaz, and Shirin Behshahr, respectively), the effect was not significant for the Malas Mumtaz variety because it already had a high rooting percentage (93.33%). The Malas Mumtaz variety also exhibited a higher number of shoots (4.32) than the other two genotypes (4.00 and 3.48 for the wild genotype and the Shirin Behshahr variety, respectively) on average across two auxin conditions. Backward multiple regression showed that rooting percentage was related to quercetin and DPPH. For the mean root length and the longest root length, the remaining variables in the models were DPPH, phenols, and flavonoids. For the number of shoots, the flavonoids, DPPH, quercetin, and gallic acid showed a significant relationship with this trait. DPPH was present in all models with a negative partial regression coefficient. However, a positive relationship of quercetin with rooting percentage and number of shoots was observed. Conclusion: This study demonstrates that adventitious rooting in pomegranate hardwood cuttings is influenced by seasonal changes of phenolic compounds in the mother plant, genotype, and the application of auxin. The Malas Mumtaz variety demonstrated a high genetic potential for rooting and showed satisfactory rooting, even in the absence of exogenous auxin treatment. Endogenous quercetin and DPPH showed a relationship with the rooting percentage and number of shoots. This finding proved the role of quercetin as a rooting enhancer in pomegranate cuttings. | ||
| کلیدواژهها | ||
| Adventitious root؛ Cutting؛ DPPH؛ Pomegranate؛ Propagation | ||
| مراجع | ||
|
Abbasi S, Mirsoleimani A, Jafari M. 2024. Diversity of phenolic compounds, antioxidant capacity, and mineral element content in four fig cultivars (Ficus carica L.) and their correlation with the rooting of hardwood cuttings. Arid Land Res Manage. 38(4): 606-623. https://doi.org/10.1080/15324982.2024.2318633
Alizadeh M, Nazari J. 2021. An introduction to the wild and feral fruits of Golestan province. Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. 558 p. (In Persian with English abstract).
Alizadeh M, Habibzadeh L. 2024. Pomegranate grafting: Optimization of technique and evaluation of fruit traits affected by cultivated and wild rootstocks. Sci Hortic. 338: 113786. https://doi.org/10.1016/j.scienta.2024.113786
Antony A, Farid M. 2022. Effect of temperatures on polyphenols during extraction. Appl Sci. 12(4): 2107. https://doi.org/10.3390/app12042107
Bagautdinova ZZ, Omelyanchuk N, Tyapkin AV, Kovrizhnykh VV, Lavrekha VV, Zemlyanskaya EV. 2022. Salicylic acid in root growth and development. Int J Mol Sci. 23(4): 2228. https://doi.org/10.3390/ijms23042228
Belkacem N, Djaziri R, Lahfa F, El-Haci IA, Boucherit Z. 2014. Phytochemical screening and in vitro antioxidant activity of various Punica granatum L. peel extracts from Algeria: A comparative study. Phytothérapie. 12: 372-379. https://doi.org/10.1007/s10298-014-0850-x
Benabdallah A, Rahmoune C, Boumendjel M, Aissi O, Messaoud C. 2016. Total phenolic content and antioxidant activity of six wild Mentha species (Lamiaceae) from northeast of Algeria. Asian Pac J Trop Biomed. 6(9): 760-766. https://doi.org/10.1016/j.apjtb.2016.06.016
Boo YC. 2019. Can plant phenolic compounds protect the skin from airborne particulate matter? Antioxidants. 8(9): 379. https://doi.org/10.3390/antiox8090379
Cheniany M, Ebrahimzadeh H, Masoudi-nejad A, Vahdati K, Leslie C. 2010. Effect of endogenous phenols and some antioxidant enzyme activities on rooting of Persian walnut (Juglans regia L.). Afr J Plant Sci. 4(12): 479-487. https://doi.org/10.5897/AJPS.9000081
Curir P, Sulis S, Mariani F, van Sumere CF, Marchesini A, Dolci M. 1993. Influence of endogenous phenols on rootability of Chamaelaucium uncinatum Schauer stem cuttings. Sci Hortic. 55(3-4): 303-314. https://doi.org/10.1016/0304-4238(93)90041-N
da Costa CT, de Almeida MR, Ruedell CM, Schwambach J, Maraschin FS, Fett-Neto AG. 2013. When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings. Front Plant Sci. 4: 133. https://doi.org/10.3389/fpls.2013.00133
De Klerk GJ, Guan H, Huisman P, Marinova S. 2011. Effects of phenolic compounds on adventitious root formation and oxidative decarboxylation of applied indoleacetic acid in Malus ‘Jork 9’. Plant Growth Regul. 63: 175-185. https://doi.org/10.1007/s10725-010-9555-9
Denaxa NK, Roussos PA, Vemmos SN. 2020. Assigning a role to the endogenous phenolic compounds on adventitious root formation of olive stem cuttings. J Plant Growth Regul. 39: 411-421. https://doi.org/10.1007/s00344-019-09991-0
Denaxa NK, Tsafouros A, Roussos PA. 2022. Role of phenolic compounds in adventitious root formation. In: Husen A (ed.) Environmental, physiological and chemical controls of adventitious rooting in cuttings. Cambridge, Massachusetts: Academic Press, pp. 251-288. https://doi.org/10.1016/B978-0-323-90636-4.00013-1
do Prado DZ, Dionizio RC, Vianello F, Baratella D, Costa SM, Lima GPP. 2015. Quercetin and indole 3-butyric acid (IBA) as rooting inducers in Eucalyptus grandis × E. urophylla. Aust J Crop Sci. 9(11): 1057-1063.
Ebrahimzadeh MA, Pourmorad F, Hafezi S. 2008. Antioxidant activities of Iranian corn silk. Turk J Biol. 32(1): 43-49.
Guranna P, Huchesh CH. 2018. In vitro regeneration in pomegranate (Punica granatum L.) cv. Bhagwa using double nodal segments. Res J Biotechnol. 13(8): 1-10.
Hammatt N. 1994. Promotion by phloroglucinol of adventitious root formation in micropropagated shoots of adult wild cherry (Prunus avium L.). Plant Growth Regul. 14: 127-132. https://doi.org/10.1007/BF00025213
Hemmaty S, Hosseinzadeh R, Dilmaghani MR, Tagiloo R, Mohseniazar M. 2011. Effect of UV-C irradiation on phenolic composition of ‘Rishbaba’ table grape (Vitis vinifera cv. Rishbaba). J Plant Physiol Breed. 1(2): 29-38.
Holland D, Hatib K, Bar-Ya’akov I. 2009. Pomegranate: botany, horticulture, breeding. In: Janick J (ed.) Hortic Rev. 35: 127-191. https://doi.org/10.1002/9780470593776.ch2
Kisiriko M, Anastasiadi M, Terry LA, Yasri A, Beale MH, Ward JL. 2021. Phenolics from medicinal and aromatic plants: Characterisation and potential as biostimulants and bioprotectants. Molecules. 26: 6343. https://doi.org/10.3390/molecules26216343
Kumar R, Meena R, Sharma BD, Saroj PL. 2018. Production technology of pomegranate in arid region. CIAH/Tech./Bull. No. 65, ICAR-Central Institute for Arid Horticulture, Bikaner, Rajasthan, India.
Mehta SK, Singh KK, Harsana AS. 2018. Effect of IBA concentration and time of planting on rooting in pomegranate (Punica granatum) cuttings. J Med Plants Stud. 6(1): 250-253.
Neyestani TR, Khalaji N. 2009. The inhibitory effects of gallic acid on the growth of bacteria β-hemolytic Streptococcus and pathogenic Escherichia coli in vitro. J Microbiol Knowl. 1(2): 11-16 (In Persian with English abstract).
Osterc G, Štefančič M, Solar A, Štampar F. 2007. Potential involvement of flavonoids in the rooting response of chestnut hybrid (Castanea crenata x Castanea sativa) clones. Aust J Exp Agric. 47: 96-102. https://doi.org/10.1071/EA05149
Owais SJ. 2010. Rooting response of five pomegranate varieties to indole butyric acid concentration and cuttings age. Pak J Biol Sci. 13: 51-58. https://doi.org/10.3923/pjbs.2010.51.58
Pratyusha S. 2022. Phenolic compounds in the plant development and defense: An overview. In: Hasanuzzaman M, Nahar K (eds.) Plant stress physiology - Perspectives in agriculture Physiology. IntechOpen. https://doi.org/10.5772/intechopen.102873
Prevc T, Šegatin N, Ulrih NP, Cigić B. 2013. DPPH assay of vegetable oils and model antioxidants in protic and aprotic solvents. Talanta. 109: 13-19. https://doi.org/10.1016/j.talanta.2013.03.046
Sarkhosh A, Yavari AM, Zamani Z (eds.). 2021. The pomegranate: Botany, production and uses. CABI Publication.
Schulz E, Tohge T, Zuther E, Fernie AR, Hincha DK. 2016. Flavonoids are determinants of freezing tolerance and cold acclimation in Arabidopsis thaliana. Sci Rep. 6: 34027. https://doi.org/10.1038/srep34027
Singh HP, Kaur S, Batish DR, Kohli RK. 2009. Caffeic acid inhibits in vitro rooting in mung bean [Vigna radiata (L.) Wilczek] hypocotyls by inducing oxidative stress. Plant Growth Regul. 57(1): 21-30. https://doi.org/10.1007/s10725-008-9314-3
Singh NV, Karimi HR, Sharma J, Babu KD. 2021. Pomegranate propagation and nursery management. In: Sarkhosh A, Yavari AM, Zamani Z (eds.) The pomegranate: Botany, production and uses. CABI Publication.
Steffens B, Rasmussen A. 2016. The physiology of adventitious roots. Plant Physiol. 170(2): 603-617. https://doi.org/10.1104/pp.15.01360
Tarragó J, Sansberro P, Filip R, López P, González A, Luna C, Mroginski L. 2005. Effect of leaf retention and flavonoids on rooting of Ilex paraguariensis cuttings. Sci Hortic. 103(4): 479-488. https://doi.org/10.1016/j.scienta.2004.07.004
Tsao R, Yang R, Young JC, Zhu H. 2003. Polyphenolic profiles in eight apple cultivars using high-performance liquid chromatography (HPLC). J Agric Food Chem. 51: 6347-6353.
ValizadehKaji B, Ershadi A, Tohidfar M. 2013. In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’. Physiol Mol Biol Plants. 19(4): 597-603. https://doi.org/10.1007/s12298-013-0193-3
Vazifeshenas M, Khayyat M, Jamalian S, Samadzadeh A. 2009. Effects of different scion-rootstock combinations on vigor, tree size, yield and fruit quality of three Iranian cultivars of pomegranate. Fruits. 64(6): 343-349. https://doi.org/10.1051/fruits/2009030
Wilson P, Staden JV. 1990. Rhizocaline, rooting co-factors, and the concept of promoters and inhibitors of adventitious rooting- a review. Ann Bot. 66(4): 479-490. https://doi.org/10.1093/oxfordjournals.aob.a088051
Zargoosh Z, Ghavam M, Bacchetta G, Tavili A. 2019. Effects of ecological factors on the antioxidant potential and total phenol content of Scrophularia striata Boiss. Sci Rep. 9: 16021. https://doi.org/10.1038/s41598-019-52605-8
Zhang Y, Cai P, Cheng G, Zhang Y. 2022. A brief review of phenolic compounds identified from plants: their extraction, analysis, and biological activity. Nat Prod Commun. 17(1): 1-14. https://doi.org/10.1177/1934578X211069721
Zhong J, Ran Q, Han Y, Gan L, Dong C. 2025. Biosynthetic mechanisms of plant chlorogenic acid from a microbiological perspective. Microorganisms. 13(5): 1114. https://doi.org/10.3390/microorganisms13051114
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