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Changes in the expression pattern of two aquaporin genes in barley shoots under salinity stress | ||
| Journal of Plant Physiology and Breeding | ||
| دوره 14، شماره 2، 2024، صفحه 223-235 اصل مقاله (828.9 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2024.62391.1339 | ||
| نویسندگان | ||
| Mojtaba Pouyanmehr1؛ Seyed Abolghasem Mohammadi* 2؛ Mahmoud Toorchi2 | ||
| 1Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51666, Iran. | ||
| 2Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51666, Iran; Center of Excellence on Cereal Molecular Breeding, University of Tabriz, Tabriz 51666, Iran. | ||
| چکیده | ||
| Objective: Barley (Hordeum vulgare L.) is an important crop with wide adaptation and tolerance to abiotic stresses. Salinity is one of the major abiotic stresses affecting crop growth and production. We assessed the changes in the expression pattern of HvTIP2;3 and HvTIP4;1 genes from the aquaporin family in the shoot of salt-tolerant cultivar Sahara3771, an advanced breeding line (A-Line), and the salt-sensitive cultivar Clipper. Methods: A split-plot experiment, based on a randomized complete block design with three replications, was conducted in a hydroponic culture system under greenhouse conditions. The studied genotypes were cultured under normal and 100 and 200 mM NaCl treatments. Twenty-four hours, three days, and three weeks after salinity treatment, leaf samples were harvested for RNA extraction. Real-time PCR was conducted using gene-specific primers, and ΔΔCt was used in the analysis of variance. Results: The results revealed significant effects of genotypes and sampling times on the expression pattern of the HvTIP4;1 gene. Salinity × genotype, salinity × sampling time, genotype × sampling time, and salinity × genotype × sampling time interactions were also significant (p < 0.01). The expression pattern of the two studied genes was not similar in the tolerant genotypes, implying different tolerance mechanisms in these genotypes. Conclusion: The studied barley genotypes showed different salt-tolerance mechanisms. These findings provide the foundation for future work to dissect the role of HvTIP2;3 and HvPIP1;4 genes, as well as other aquaporins containing tonoplast intrinsic protein (HvTIP) coding genes, in conferring adaptation to various stresses. | ||
| کلیدواژهها | ||
| Advanced breeding line؛ Aquaporin؛ Gene expression؛ Genotype؛ RT-PCR | ||
| مراجع | ||
|
Afzal M, Hindawi SES, Alghamd SS, Migdadi HH, Altaf Khan M, Hasnain MU, Arslan M, ur Rahman MH, Muhammad MS. 2023. Potential breeding strategies for improving salt tolerance in crop plants. J Plant Growth Regul. 42: 3365-3387. https://doi.org/10.1007/s00344-022-10797-w
Aharon R, Shahak Y, Wininger S, Bendov R, Kapulnik Y, Galili G. 2003. Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress. Plant Cell. 15(2): 439-447. https://doi.org/10.1105/tpc.009225
Alavilli H, Awasthi JP, Rout GR, Sahoo L, Lee B-H, Panda SK. 2016. Overexpression of a barley aquaporin gene, HvPIP2;5 confers salt and osmotic stress tolerance in yeast and plants. Front Plant Sci. 7: 1566. https://doi.org/10.3389/fpls.2016.01566. https://doi.org/ 10.1105/tpc.009225
Allbed A, Kumar L. 2013. Soil salinity mapping and monitoring in arid and semi-arid regions using remote sensing technology: a review. Adv Remote Sens. 2: 373-385. http://dx.doi.org/10.4236/ars.2013.24040
Beltrán JM. 1999. Irrigation with saline water: benefits and environmental impact. Agric Water Manag. 40(2-3): 183-194. https://doi.org/10.1016/S0378-3774(98)00120-6
Atta K, Mondal S, Gorai S, Singh AP, Kumari A, Ghosh T, Roy A, Hembram S, Gaikwad DJ, Mondal S, et al. 2023. Impacts of salinity stress on crop plants: improving salt tolerance through genetic and molecular dissection. Front Plant Sci. 14: 1241736. https://doi.org/10.3389/fpls.2023.1241736
Boursiac Y, Chen S, Luu D-T, Sorieul M, van den Dries N, Maurel C. 2005. Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression. Plant Physiol. 139(2): 790-805. https://doi.org/10.1104/pp.105.065029
Chaves MM, Flexas J, Pinheiro C. 2009. Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann Bot. 103(4): 551-560. https://doi.org/10.1093/aob/mcn125
EL Sabagh A, Hossain A, Shohidul Islam M, Barutcular C, Hussain S, Hasanuzzaman M, AkramT, Mubeen M, Nasim W, Fahad S, et al. 2019. Drought and salinity stresses in barley: consequences and mitigation strategies. Aust J Crop Sci. 13(6): 810-820. https://doi.org/10.21475/ajcs.19.13.06.p1286
Flowers TJ. 2004. Improving crop salt tolerance. J Exp Bot. 55(396): 307-319. https://doi.org/10.1093/jxb/erh003
Forrest KL, Bhave M. 2008. The PIP and TIP aquaporins in wheat form a large and diverse family with unique gene structures and functionally important features. Funct Integr Genomics. 8: 115-133. https://doi.org/10.1007/s10142-007-0065-4
Hove RM, Ziemann M, Bhave M. 2015. Identification and expression analysis of the barley (Hordeum vulgare L.) aquaporin gene family. PLoS One. 10(6): e0128025. https://doi.org/10.1371/journal.pone.0128025
Johanson U, Karlsson M, Johansson I, Gustavsson S, Sjövall S, Fraysse L, Weig AR, Kjellbom P. 2001. The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants. Plant Physiol. 126(4): 1358-1369. https://doi.org/10.1104/pp.126.4.1358
Karakousis A, Barr AR, Kretschmer JM, Manning S, Jefferies SP, Chalmers KJ, Islam AKM, Langridge P. 2003. Mapping and QTL analysis of the barley population Clipper × Sahara. Aust J Agric Res. 54(12): 1137-1140. https://doi.org/10.1071/AR02180
Kawasaki S, Borchert C, Deyholos M, Wang H, Brazille S, Kawai K, Galbraith , Bohnert HJ. 2001. Gene expression profiles during the initial phase of salt stress in rice. Plant Cell. 13(4): 889-905. https://doi.org/10.1105/tpc.13.4.889.
Kim H, Jeong H, Jeon J. Bae S. 2016. Effects of irrigation with saline water on crop growth and yield in greenhouse cultivation. Water. 8(4): 127. https://doi.org/10.3390/w8040127.
Kirch, H-H, Vera-Estrella R, Golldack D, Quigley F, Michalowski CB, Barkla BJ, Bohnert HJ. 2000. Expression of water channel proteins in Mesembryanthemum crystallinum. Plant Physiol. 123(1): 111-124. https://doi.org/10.1104/pp.123.1.111.
Kurowska MM, Wiecha K, Gajek K, Szarejko I. 2019. Drought stress and re-watering affect the abundance of TIP aquaporin transcripts in barley. Plos One. 14(12): e0226423. https://doi.org/10.1371/journal.pone.0226423.
Li L-G, Li S-F, Tao Y, Kitagawa Y. 2000. Molecular cloning of a novel water channel from rice: its products expression in Xenopus oocytes and involvement in chilling tolerance. Plant Sci. 154(1): 43-51. https://doi.org/10.1016/S0168-9452(99)00269-1
Ligaba A, Katsuhara M, Shibasaka M, Djira G. 2011. Abiotic stresses modulate expression of major intrinsic proteins in barley (Hordeum vulgare). C R Biol. 334(2): 127-139. https://doi.org/10.1016/j.crvi.2010.11.005.
Roy SJ, Negrão S, Tester M. 2014. Salt resistant crop plants. Curr Opin Biotechnol. 26: 115-124. https://doi.org/10.1016/j.copbio.2013.12.004.
Salami R, Mohammadi SA, Ghaffariyan S, Moghaddam M. 2017. Evaluating the expression of HvTIP2;3 and HvTIP4;1 in barley genotypes under different levels of salinity stress. Indian J Genet Plant Breed. 77(04): 524-530. https://doi.org/10.5958/0975-6906.2017.00069.4.
Shi L-N, Lu L-X, Ye J-R, Shi H-M. 2022. The endophytic strain ZS-3 enhances salt tolerance in Arabidopsis thaliana by regulating photosynthesis, osmotic stress, and ion homeostasis and inducing systemic tolerance. Front Plant Sci. 13: 820837. https://doi.org/10.3389/fpls.2022.820837
Suga S, Komatsu S, Maeshima M. 2002. Aquaporin isoforms responsive to salt and water stresses and phytohormones in radish seedlings. Plant Cell Physiol. 43(10): 1229-1237. https://doi.org/10.1093/pcp/pcf148
Sun Q, Liu X, Kitagawa Y, Calamita G, Ding X. 2024. Plant aquaporins: their roles beyond water transport. Crop J. https://doi.org/10.1016/j.cj.2024.04.005
Walia H, Wilson C, Condamine P, Liu X, Ismail AM, Close TJ. 2007. Large‐scale expression profiling and physiological characterization of jasmonic acid‐mediated adaptation of barley to salinity stress. Plant Cell Environ. 30(4): 410-421. https://doi.org/10.1111/j.1365-3040.2006.01628.x.
Wang X, Li Y, Ji W, Bai X, Cai H, Zhu D, Sun X-L, Chen L-J, Zhu Y-M. 2011. A novel Glycine soja tonoplast intrinsic protein gene responds to abiotic stress and depresses salt and dehydration tolerance in transgenic Arabidopsis thaliana. J Plant Physiol. 168(11): 1241-1248. https://doi.org/10.1016/j.jplph.2011.01.016
Yamada S, Katsuhara M, Kelly WB, Michalowski CB, Bohnert HJ. 1995. A family of transcripts encoding water channel proteins: tissue-specific expression in the common ice plant. Plant Cell. 7(8): 1129-1142. https://doi.org/10.1105/tpc.7.8.1129
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