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Exploring the needle’s morphological, phytochemical, and genetic diversity of Taxus baccata L. in northern Iran | ||
| Journal of Plant Physiology and Breeding | ||
| دوره 14، شماره 2، 2024، صفحه 193-221 اصل مقاله (1.21 M) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2024.20094 | ||
| نویسندگان | ||
| Esmail Hedati؛ Azim Ghasemnezhad* ؛ Mostafa Khoshhal Sarmast؛ Amir Ghorbanzadeh | ||
| Department of Horticultural Science, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. | ||
| چکیده | ||
| Objective: Golestan province, located in northern Iran, is recognized as one of the most important gene pools of the Yew tree (Taxus baccata L.) worldwide. Despite its significance, there is limited information on the diversity of yew populations in this region. This study aimed to investigate the diversity of these populations by examining morphological, phytochemical, and genetic traits. Methods: To carry out the experiment, a completely randomized design with three replications was employed during the summer of 2020 in the Gorgan University of Agricultural Sciences and Natural Resources< Gorgan, Iran. Young shoots were collected from 30 individuals across 10 populations. Morphological traits of needles were measured using digital scales and calipers, while phytochemical properties, including phenolics, flavonoids, alkaloids, antioxidant activities, and taxol concentration, were analyzed using standard biochemical and HPLC methods. Genetic variation was evaluated through PCR using inter-simple sequence repeat (ISSR) markers. Data from morphological and phytochemical analyses were statistically processed with SPSS, whereas genetic data were evaluated using NTSYS and GenAlex software. Results: Morphological analyses revealed considerable variability in traits among different yew populations, allowing for their classification into distinct groups. However, no significant variation was observed in taxol content among the sampled trees, although taxol presence indicates a promising potential for production. Differences in leaf antioxidant activity were observed among populations, suggesting varying levels of phytochemical activity. Using five ISSR primers highlighted genetic diversity among the populations. However, the majority of the genetic diversity (84%) was attributed to variations within populations, while only 16% was due to differences among populations. These findings enhance our understanding of the genetic diversity present within yew populations in the Golestan province and underscore the importance of conserving this diversity. Maintaining genetic variation is essential for the long-term adaptability, resilience, and survival of these populations. This study provides critical insights for developing sustainable management practices and highlights the potential medicinal and economic value inherent in the yew populations of this region. Conclusion: Our investigation revealed no significant variation in taxol content among yew populations in the Golestan region, Iran. However, morphological, phytochemical, and genetic analyses showed significant differences among populations, highlighting the potential for taxol production and the importance of conserving genetic diversity for sustainable management. | ||
| کلیدواژهها | ||
| Antioxidant؛ Genetic diversity؛ ISSR؛ Morphology؛ Taxol؛ Yew | ||
| مراجع | ||
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