آمار
| تعداد نشریات | 45 |
| تعداد شمارهها | 1,391 |
| تعداد مقالات | 17,025 |
| تعداد مشاهده مقاله | 54,912,906 |
| تعداد دریافت فایل اصل مقاله | 17,413,301 |
Evaluation of mother-plant growing beds, explant type, and different disinfection treatments in control of Sansevieria rhizome contamination under in vitro conditions | ||
| Journal of Plant Physiology and Breeding | ||
| دوره 14، شماره 2، اسفند 2024، صفحه 23-38 اصل مقاله (653.63 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2024.63310.1346 | ||
| نویسندگان | ||
| Matin Kazemzadeh Bahnamirei1؛ Mostafa K. Sarmast* 2؛ Mohammad Naghi Padasht Dahkaei3؛ Mahdi Alizadeh2 | ||
| 1Department of Horticultural Science and Landscape Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.Agricultural Sciences and Natural Resources (GUASNR), Gorgan, Golestan, Iran | ||
| 2Department of Horticultural Science and Landscape Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. | ||
| 3Guilan Agricultural and Natural Resources Research and Education Center, AREEO, Rasht, Iran. | ||
| چکیده | ||
| Objective: Cloning ornamental plants, particularly chimeric varieties, is a crucial strategy for enhancing competitiveness in the ornamental plant market. Since the only method to propagate clones of chimeric Sansevieria varieties is through offset production from rhizome origins, optimizing sterilization techniques for in vitro rhizome explants is imperative. A significant drawback of plant tissue culture is the risk of microbial contamination, making the removal of contaminants a foundational step for successful tissue culture. Initial experiments on Sansevieria trifasciata rhizome explants revealed that conventional disinfectants were ineffective at eliminating contamination. Consequently, this study aimed to improve culture conditions by evaluating various factors essential for successful plant tissue culture. Methods: In a preliminary experiment, two types of mother-plant growing media (perlite and conventional greenhouse soil) were compared using a t-test with 50 experimental units within a group. The second experiment was conducted on perlite as the mother-plant growing medium using a factorial arrangement with three factors based on the completely randomized design with three replications. The factors included three rhizome explant types (single node, terminal meristem, and rhizomes with terminal buds), benomyl and carbendazim fungicides at two concentrations of 2% and 10%, and mercuric chloride as the chemical disinfectant at two concentrations of 0.1% and 0.2% for 2 and 20 minutes plus a control without mercuric chloride. Results: Results indicated that culturing mother plants in the perlite bed significantly reduced microbial loads. The results of the second experiment showed that treatment with 10% benomyl fungicide effectively diminished microbial contamination. Among the disinfectants tested, a 20-minute treatment of explants with 0.2% mercuric chloride resulted in the lowest fungal contamination. Among the explant types, single-node explants exhibited the least fungal contamination. Conclusion: pre-treating single-node rhizome explants with 10% benomyl fungicide, followed by a 2-minute exposure to 70% alcohol and a 20-minute treatment with 0.2% mercuric chloride in a laminar flow cabinet, leads to optimal microbial decontamination. These findings underscore the importance of specific sterilization protocols in enhancing the efficacy of plant tissue culture methods for chimeric Sansevieria propagation. | ||
| کلیدواژهها | ||
| Benomyl؛ Disinfection؛ Explant؛ Homogenous growth؛ Mercuric chloride؛ Rhizome؛ Tissue culture | ||
| مراجع | ||
|
Ahmadi MR, Shahhoseini R, Hakimi L. 2022. Micropropagation of Iranian native oregano (Origanum vulgare L.) using growth regulators. J Plant Physiol Breed. 12(2): 105-116. https://doi.org/10.22034/JPPB.2022.16376
Altan F, Bürün B, Sahin N. 2010. Fungal contaminants observed during micropropagation of Lilium candidum L. and the effect of chemotherapeutic substances applied after sterilization. Afr J Biotechnol. 9(7): 991-995. https://doi.org/10.5897/AJB08.090
Arnold MA. 2004. Landscape plants for Texas and environments, Third Edition. USA: Stipes Publishing.
Bach A, Sochaki D. 2012. Propagation of ornamental geophytes: physiology and management systems. In: Kamenetsky R, Okubo H. (eds.). Ornamental geophytes from basic science to sustainable production. Boca Raton, Florida: CRC Press, pp. 261-287. https://doi.org/10.1201/b12881
Byrom MH. 1950. Progress with long vegetable fibres crops in peace and war. The Year Book of Agriculture. US Department of Agriculture, Washington DC: U.S. Government Printing Office.
Carey DJ, Whipker BE, McCall I, Buhler, W. 2008. Benzyl adenine foliar sprays increase offsets in Sempervivum and Echeveria. J Hort Sci. 53: 19-21.
Dane F, Dalgiç O. 2005. The effects of fungicide benomyl (benlate) on growth and mitosis in onion (Allium cepa L.) root apical meristem. Acta Biol Hung. 56(1- 2): 119-128. https://doi.org/10.1556/ABiol.56.2005.1-2.12
Daud NH, Jayaraman S, Mohamed R. 2012. An improved surface sterilization technique for introducing leaf, nodal and seed explants of Aquilaria malaccensis from field sources into tissue culture. Asia Pac J Mol Biol Biotechnol. 20(2): 55-58.
Dolati M, Abbasabad A, Seyfi E, Alizadeh M, Khoshhal Sarmast M. 2023. Appraisal of leaf cutting, soil mixture and leaf explants on production of Sansevieria trifasciata under ex/in vitro condition. Flower and Ornamental Plants. 7(2): 261-276 (In Persian with English abstract). https://doi.org/10.61186/flowerjournal.7.2.261
García-Hernández E, Loera-Quezada MM, Morán-Velázquez DC, López MG, Chable-Vega MA, Santillán-Fernández A, Zavaleta-Mancera HA, Tang JZ, Azadi P, Ibarra-Laclette E, et al. 2022. Indirect organogenesis for high frequency shoot regeneration of two cultivars of Sansevieria trifasciata Prain differing in fiber production. Sci Rep. 12: 8507 https://doi.org/10.1038/s41598-022-12640-4
Ma X, Gang DR. 2006. Metabolic profiling of in vitro micropropagated and conventionally greenhouse grown ginger (Zingiber officinale). Phytochemistry. 67(20): 2239–2255. https://doi.org/10.1016/j.phytochem.2006.07.012
Maloupa E, Mitsios I, Martinez PF, Bladenopoulou S. 1992. Study of substrates used in gerbera soilless culture grown in plastic greenhouse. Acta Hortic. 323: 139-144. https://doi.org/10.17660/ActaHortic.1993.323.12
Mamza WS, Zarafi AB, Alabi O. 2010. In vitro evaluation of six fungicides on radial mycelial growth and regrowth of Fusarium pallidoroseum isolated from castor (Ricinus communis) in Samaru, Nigeria. Arch Phytopathol Plant Prot. 43(2): 116-122. https://doi.org/10.1080/03235400701806401
Marinescu MV, Teodorescu A, Şuţan NA. 2013. Preliminary results on the in vitro propagation by leaf explants and axillary buds of Iris aphylla L. J Hortic For Biotehnol. 17: 279-282.
Mihaljević I, Dugalić K, Tomaš V, Viljevac M, Pranjić A, Čmelik Z, Puškar B, Jurković Z. 2013. In vitro sterilization procedures for micropropagation of ‘Oblačinska’ sour cherry. J Agric Sci. 58(2): 117-126. https://doi.org/10.2298/JAS1302117M
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15: 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nazary Moghaddam Aghayeh R, Abedy B, Balandari A, Samiei L, Tehranifar A. 2019). Contamination control in Iranian seedless barberry micropropagation. J Plant Physiol Breed. 9(1): 97-110. https://doi.org/10.22034/jppb.2019.10387
Ngezahayo E, Liu B. 2014. Axillary bud proliferation approach for plant biodiversity conservation and restoration. Int J Biodivers. 2014: 27025. https://doi.org/10.1155/2014/727025
Podwyszyńska M. 2012. The mechanism of in vitro storage organ formation in ornamental geophytes. Floriculture Ornamental Biotechnology. 1(6): 9-23.
Read PE, Preece JE. 2014. Cloning: Plants – Micropropagation/tissue culture. In: Van Alfen N (ed.). Encyclopedia of Agriculture and Food Systems. 2: 317-336. San Diego, CA, USA: Academic Press. https://doi.org/10.1016/b978-0-444-52512-3.00224-2
Reed BM, Tanprasert P. 1995. Detection and control of bacterial contaminants of plant tissue cultures. A review of recent literature. Plant Tissue Cult Biotechnol. 1(3): 137-142.
Sarmast MK. 2018. In vitro propagation of conifers using mature shoots. J For Res. 29: 565-574. https://doi.org/10.1007/s11676-018-0608-7
Sawant IS, Wadkar PN, Ghule SB, Rajguru YR, Salunkhe VP, Sawant, SD. 2017. Enhanced biological control of powdery mildew in vineyards by integrating a strain of Trichoderma afroharzianum with sulphur. Biol Control. 114: 133-143. https://doi.org/10.1016/j.biocontrol.2017.08.011
Tarinejad A, Amiri S. 2019. Influence of plant growth regulators, carbohydrate source and concentration on micropropagation and other physiological traits of grape (Vitis vinifera L. cv. Shahroudi) under in vitro conditions. J Plant Physiol Breed. 9(1): 75-82. https://doi.org/10.22034/JPPB.2019.10378
Teixeira da Silva JA, Winarto B, Dobránszki J, Zeng S. 2015. Disinfection procedures for in vitro propagation of anthurium. Folia Hortic. 27: 3-14. https://doi.org/10.1515/fhort-2015-0009
Wolverton BC, Johnson A, Bounds K. 1989. Interior landscape plants for indoor air pollution abatement. NASA. ID: 19930073077.
Yang BM, Huang YL, Xu WJ, Bao LX. 2015. Explant selection and cluster buds induction in vitro of saffron (Crocus sativus L.). Agric Sci Technol Commun. 2: 106-108.
Yasmin S, Nehvi FA, Wani SA. 2013. Tissue culture as an alternative for commercial corm production in saffron: a heritage crop of Kashmir. Afr J Biotechnol. 12(25): 3940-3946. https://doi.org/10.5897/AJB2013.12378
| ||
|
آمار تعداد مشاهده مقاله: 126 تعداد دریافت فایل اصل مقاله: 111 |
||