Evaluation of the ability of rhizosphere isolates to solubilize low-soluble Zn under in-vitro conditions and their ability to supply Zn to maize | ||
| دانش کشاورزی وتولید پایدار | ||
| Volume 32, Issue 3, June 2022, Pages 183-199 PDF (995.81 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22034/saps.2022.49165.2776 | ||
| Authors | ||
| Bahman Khoshru* 1; Mohammad Reza Sarikhani2; Adel Reyhanitabar3; Shahin Oustan4; Mohammad Ali Malbobi5 | ||
| 1Tabriz University | ||
| 2Department of Soil Science-University of Tabriz | ||
| 3- Department of Soil Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran | ||
| 4Department of Soil Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran | ||
| 5National Institute of Genetic Engineering and Biotechnology, Department of Plant Biotechnology, Tehran, Iran. | ||
| Abstract | ||
| Background & Objective: In this study, the effect of potent zinc solubilizing bacteria on zinc supply of single cross cultivar 704 was evaluated. Materials and Methods: Isolation of bacteria from rhizospheres of maize, wheat and sunflower was performed in different cities of East Azerbaijan province. The experiment was performed in two phases in vitro and in a greenhouse in a completely randomized statistical design with three replications. Results: A total of 20 bacterial isolates were obtained from the rhizosphere of the studied plants. In qualitative evaluation, the results showed that ZP13 isolates in zinc phosphate source, ZO11 in zinc oxide source and ZC10 in zinc carbonate source with HD / CD ratio with 1.74, 1.68 and 1.61, respectively, had the highest solubility. In quantitative evaluation of zinc solubility, ZP13 isolate (24.64 mg / L), ZC10 isolate in zinc carbonate (19.48 mg / L) and ZO11 isolate in zinc oxide (26.54 mg / L) had the highest solubility. The two isolates ZO11 and ZO14 in comparison with the negative control treatment led to an increase in zinc uptake of 179.7 and 62.37% in the root and 155.1 and 110.6% in the shoot part of maize, respectively. Identification of isolates ZO11 and ZO14 showed that they belong to Acinetobacter calcoaceticus and Agromyces italicus, respectively. Conclusion: According to the results of this experiment, the potential of rhizobacteria of different plants can be used to provide the zinc required by plants as a healthy and eco-friendly solution. | ||
| Keywords | ||
| Zinc solubilizing bacteria; Siderophore; Organic acids; pH reduction; Zinc compounds | ||
| References | ||
|
Bapiri A, Asgharzadeh A, Mujallali H, Khavazi K and Pazira E. 2012. Evaluation of zinc solubilization potential by different strains of Fluorescent Pseudomonads. Journal of Applied Sciences and Environmental Management, 16(3): 147-153.
Bharucha UD, Patel KC, Trivedi UB. 2013. In vitro screening of isolates for its plant growth promoting activities from the rhizosphere of Alfalfa (Medicago sativa). Journal of Microbiology and Biotechnology Research, 3(5):79-88.
Biari A, Golami A. and Rahmani HA. 2008. Growth promoting and enhanced nutrient uptake of maize (Zea mays L.) by application of plant growth promoting rhizobacteria in arid region of Iran. Australian Journal of Biological Sciences, 8: 1015–1020.
Boratyn GM, Camacho C, Cooper PS, Coulouris G, Fong A, Ma N, Madden TL, Matten WT, Mc Ginnis SD, Merezhuk Y and Raytselis Y. 2013. BLAST: a more efficient report with usability improvements. Nucleic Acids Research, 41:W29-W33.
Bric JM, Bostock RM and Silverstone SE. 1991. Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Applied and Environmental Microbiology, 57(2): 535-538.
Cala-Rivero V, De La Flor Masedo M and Vigil De La Villa R. 1999. Effect of soil properties on zinc retention in agricultural calcareous soils. Agrochimica, 43(1): 46-54.
Castillo-González J, Ojeda-Barrios D, Hernández-Rodríguez A and González-Franco AC. 2018. Robles-Hernández L, López-Ochoa GR. Zinc metalloenzymes in plants. Interciencia, 43(4):242-248.
Castillo-González J, Ojeda-Barrios D, Hernández-Rodríguez A, González-Franco AC, Robles-Hernández L, López-Ochoa GR. 2018. Zinc metalloenzymes in plants. Interciencia, 43(4):242-248.
Dinesh R, Anandaraj M, Kumar A, Bini YK, Subila KP and Aravind R. 2015. Isolation, characterization, and evaluation of multi-trait plant growth promoting rhizobacteria for their growth promoting and disease suppressing effects on ginger. Microbiological Research, 173: 34-43.
Dinesh R, Srinivasan V, Hamza S, Sarathambal C, Anke Gowda SJ, Ganeshamurthy AN, Gupta SB, Aparna Nair V, Subila KP, Lijina A and Divya VC. 2018. Isolation and characterization of potential Zn solubilizing bacteria from soil and its effects on soil Zn release rates, soil available Zn and plant Zn content. Geofisica Internacional, 321:173-186.
Eleiwa ME, Hamed ER and Shehata HS. 2012. The role of biofertilizers and/or some micronutrients on wheat plant (Triticum aestivum L.) growth in newly reclaimed soil. Journal of Medicinal Plants Research, 6(17):3359-69.
Fess TL and Benedito VA. 2018. Organic versus conventional cropping sustainability: A Comparative System Analysis. Sustainability, 10(1):272-281.
Gontia-Mishra I, Sapre S and Tiwari S. 2017. Zinc solubilizing bacteria from the rhizosphere of rice as prospective modulator of zinc biofortification in rice. Rhizosphere, 3:185-190.
Goteti PK, Emmanuel LDA, Desai S and Shaik MHA. 2013. Prospective zinc solubilizing bacteria for enhanced nutrient uptake and growth promotion in maize (Zea mays L.). International journal of Microbiology, 7(2): 421-432.
Gregory PJ, Wahbi A, Adu-Gyamfi J, Heiling M, Gruber R, Joy EJ and Broadley MR. 2017. Approaches to reduce zinc and iron deficits in food systems. Global Food Security, 17 (4): 217-224.
Hamidpour M, Afyuni M, Khadivi E, Zorpas A and Inglezakis V. 2012. Composted municipal waste effect on chosen properties of calcareous soil. International Agrophysics, 26(4):365-374.
Hu X, Chen J and Guo J. 2006. Two phosphate-and potassium-solubilizing bacteria isolated from Tianmu Mountain, Zhejiang, China. World journal of Microbiology and Biotechnology, 22(9): 983-990.
Hübner C and Haase H. 2021. Interactions of zinc-and redox-signaling pathways. Redox Biology, 41:101916.
Hussain A, Arshad M, Zahir ZA and Asghar M. 2015. Prospects of zinc solubilizing bacteria for enhancing growth of maize. Pakistan journal of Agricultural Sciences, 52(4): 18-26.
Idayu O, Maizatul N, Radziah O, Halimi MS and Edaroyati MW. 2017. Inoculation of zinc-solubilizing bacteria with different zinc sources and rates for improved growth and zinc uptake in rice. International Journal of Agriculture and Biology, 19(5):1137-1140.
Khoshru B, Mitra D, Khoshmanzar E, Myo EM, Uniyal N, Mahakur B, Mohapatra PK, Panneerselvam P, Boutaj H, Alizadeh M, Cely MV. 2020a. Current scenario and future prospects of plant growth-promoting rhizobacteria: an economic valuable resource for the agriculture revival under stressful conditions. Journal of Plant Nutrition 30:1-31.
Khoshru B, Mitra D, Mahakur B, Sarikhani M R, Mondal R, Verma D and Pant K. 2020b. Role of soil rhizobacteria in utilization of an indispensable micronutrient zinc for plant growth promotion. Journal of Natural Remedies, 21(6): 47-58.
Klute A. 1986. Methods of soil analysis. Part I: physical and mineralogical methods. ASA, Inc. SSSA Inc. Madison, Wisconsin USA.
Malakoti MJ. 2001. Sustainable Agriculture and Yield Increase by Optimizing Fertilizer Consumption in Iran, Third Edition, Sana Publications, Tehran, Iran.
Nelson DW and Sommers L. 1982. Total carbon, organic carbon, and organic matter (Pp. 539-579). Methods of soil analysis. Part 2. Chemical and microbiological properties. 2nd Ed. Argon. Mongor. No. 9. ASA and SSSA, Madison.
Olsen SR, Sommer LE. 1982. Phosphorus (Pp. WI. 403-430). In: Klute A (Ed). Methods of Soil Analysis: Chemical and Microbiological Properties, part 2. 2nd Ed. Argon. Mongor. No. 9. ASA and SSSA, Madison.
Rengel Z. 2015. Availability of Mn, Zn and Fe in the rhizosphere. Journal of Soil Science and Plant Nutrition, 15(2):397-409.
Reyhani Tabar A, Najafali Karimian M, Moez Ardalan S and Savaqhebi Gh. 2007. Zn adsorption properties in some calcareous soils of Iran, 10th Iranian Soil Science Congress, Karaj, Agricultural and Natural Resources Campus, University of Tehran. (In Persian).
Sadaghiani MR, Barin M and Jalili F. 2008. The Effect of PGPR inoculation on the growth of wheat. International Meeting on Soil Fertility Land Management and Agroclimatology, Turkey, 891– 898.
Sambrook JDW and Russell. 2001. Molecular Cloning: A Laboratory Manual, vol. 1 Cold Spring Harbor, New York.
Saravanan VS, Kalaiarasan P, Madhaiyan M and Thangaraju M. 2007a. Solubilization of insoluble zinc compounds by Gluconacetobacter diazotrophicus and the detrimental action of zinc ion (Zn2+) and zinc chelates on root knot nematode Meloidogyne incognita. Letters in Applied Microbiology, 44(3): 235-241.
Saravanan VS, Madhaiyan M and Thangaraju M. 2007b. Solubilization of zinc compounds by the diazotrophic, plant growth promoting bacterium Gluconacetobacter diazotrophicus. Chemosphere, 66(9):1794-1798.
Sarikhani MR, Khoshru B and Greiner R. 2019. Isolation and identification of temperature tolerant phosphate solubilizing bacteria as a potential microbial fertilizer. World Journal of Microbiology and Biotechnology, 35(8):126-137.
Sarikhani MR, Oustan S, Ebrahimi M and Aliasgharzad N. 2018. Isolation and identification of potassium‐releasing bacteria in soil and assessment of their ability to release potassium for plants. European Journal of Soil Science, 69(6): 1078-1086.
Schwyn B and Neilands JB. 1987. Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry, 160(1):47-56.
Shahbazi K and Besharati H. 2013. Overview of agricultural soil fertility status of Iran. Land Management Journal, 1:1-15.
Sperber JI. 1958. Solution of apatite by soil microorganisms producing organic acids. Australian Journal of Agricultural Research, 9(6):782-787.
Tariq M, Hameed S, Malik KA and Hafeez FY. 2007. Plant root associated bacteria for zinc mobilization in rice. Pakistan Journal of Botany, 39(1): 245-253.
Thomas GW. 1982. Exchangeable cations (Pp. 159-165). Methods of soil analysis. Part 2. Chemical and Microbiological Properties.
Udo EJ, Bohn HL and Tucker TC. 1970. Zinc adsorption by calcareous soils. Soil Science Society of America Journal. 1970 May; 34(3):405-417.
Vaid SK, Kumar B, Sharma A, Shukla AK and Srivastava PC. 2014. Effect of Zn solubilizing bacteria on growth promotion and Zn nutrition of rice. Journal of Soil Science and Plant Nutrition, 14(4):889-910.
Waling I, Vark WV, Houba VJG and Van der Lee JJ. 1989. Soil and plant analysis, a series of syllabi, part 7: Plant analysis procedures. Wageningen Agriculture University.
Whiting SN, de Souza MP, Terry N (2001) Rhizosphere bacteria mobilize Zn for hyperaccumulation by Thlaspi caerulescens. Environmental Science and Technology, 35(15):3144-50.
Wu SC, Luo YM, Cheung KC and Wong MH. 2006. Influence of bacteria on Pb and Zn speciation, mobility and bioavailability in soil: a laboratory study. Environmental Pollution, 144(3):765-773.
Zhang W, Zheng C, Song Z, Deng A and He Z. 2015. Farming systems in China: Innovations for sustainable crop production. In Crop Physiology (Second Edition), 43-64.
Zhu H, Yang H. 2015. Isolation and characterization of a highly siderophore producing Bacillus subtilis strain (Pp. 83-9). In: Advances in Applied Biotechnology. Springer Berlin Heidelberg. | ||
|
Statistics Article View: 1,219 PDF Download: 779 |
||