آمار
| تعداد نشریات | 45 |
| تعداد شمارهها | 1,513 |
| تعداد مقالات | 18,465 |
| تعداد مشاهده مقاله | 60,266,622 |
| تعداد دریافت فایل اصل مقاله | 21,271,793 |
Impacts of cold stress on some physio-biochemical characteristics of three lines/varieties of lentils | ||
| Journal of Plant Physiology and Breeding | ||
| دوره 11، شماره 2، اسفند 2021، صفحه 61-72 اصل مقاله (561.11 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2021.14533 | ||
| نویسندگان | ||
| Seyed Mahdi Razavi* 1؛ Mahtab Javadi1؛ Hossein Mostafaei2؛ Parisa Nasrollahi1 | ||
| 1Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 2Researcher, Crop Sciences Research Division, Research Center for Agriculture and Natural Resources, Ardabil, Iran | ||
| چکیده | ||
| Lentil is one of the arctic legumes which can be damaged by the severe cold. In this regard, determination of the cold tolerance among different lentil lines or varieties has considerable importance. To evaluate Bilesovar and Precoz varieties and ILL-2580 line of lentils for cold tolerance and seed yield, a field experiment was carried out as a randomized complete block design with three replications. Also, to study the physiological and biochemical responses of the lentil plants to cold conditions, a factorial experiment was conducted in the greenhouse based on a completely randomized design. The factors were cold conditions and lentil genotypes. The results showed that the highest plant height, 100-seed weight, seed yield, and the shoot dry and fresh weight were observed in the Bilesovar variety followed by Precoz. Also, the highest percentage of cold damage was observed in the ILL-2580 line. The cold damage was 80% in ILL-2580 but it was about 41 and 32% for the Bilesovar and Precoz, respectively. The results also indicated that the chlorophyll index (SPAD) declined in Billesovar and ILL-2580 under the cold stress conditions, whereas it enhanced in Precoz (252% over the control). Moreover, the proline content was significantly increased by about 300% in the cold-treated Percoz plants as compared to the control plants. The activity of the catalase, as an antioxidant enzyme, drastically increased in Precoz and Bilesavar under cold treatment (20 and 73%, respectively over the related controls). Billesovar showed a significant increase in total flavonoids and anthocyanin content of the leaves at cold conditions. The results of SDS PAGE analysis showed that in Bilesavar and Precoz, some characteristic bands with molecular weights of 30-40 and 80-90 KD appeared in the cold-treated plants that were absent in the control plants. It can be concluded that the Bilesovar of lentils might be considered a cold-tolerant variety. On the other hand, Precoz and ILL-2580 can be regarded as semi-tolerant and cold-sensitive genotypes, respectively. | ||
| کلیدواژهها | ||
| Biochemical characteristics؛ Cold tolerance؛ Lentil؛ Physiology | ||
| مراجع | ||
|
Al-Whaibi MH, 2011. Plant heat-shock proteins: a mini review. Journal of King Saud University - Science 23(2): 139-150.
Anonymous, 2011. Legume International Nurseries and Trials. International Center for Agricultural Research in Dry Areas (ICARDA). http://www.icarda.org.
Banerjee A, Wani SH, and Roychoudhury A, 2017. Epigenetic control of plant cold responses. Frontiers in Plant Science 8: 1643.
Baek KH and Skinner DZ, 2012. Production of reactive oxygen species by freezing stress and the protective roles of antioxidant enzymes in plants. Journal of Agricultural Chemistry and Environment 1(1): 34-40.
Bates LS, Waldren RP, and Teare ID, 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39: 205-207.
Brik Y, Harpaz I, Ishaaya I, and Bondi A, 1962. Studies on the proteolytic activity of the beetles Tenebrio and Tribolium. Journal of Insect Physiology 8; 417-429.
Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1): 248-254.
Cakmak I, and Marschner H, 1992. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiology 98(4): 1222-1227.
Das K and Roychoudhury A, 2014. Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science 2: 53.
Hassan MA, Xiang C, Farooq M, Muhammad N, Yan Z, Hui X, Yuanyuan K, Bruno AK, Lele Z, and Jincai L, 2021. Cold stress in wheat: plant acclimation responses and management strategies. Frontiers in Plant Science 12: 676884.
Hayat S, Hayat Q, Alyemeni MN, Wani AS, Pichtel J, and Ahmed A, 2012. Role of proline under changing environments: a review. Plant Signalling & Behaviour 7(11): 1456-1466.
Heidarvand L and Maali Amiri R, 2010. What happens in plant molecular responses to cold stress? Acta Physiologiae Plantarum 32: 419-431.
Kalisz A, Jezdinský A, Pokluda R, Sękara, A, Grabowska A, and Gil J, 2016. Impacts of chilling on photosynthesis and chlorophyll pigment content in juvenile basil cultivars. Horticulture, Environment, and Biotechnology 57(4): 330-339.
Kumagai E, Araki T, and Kubota F, 2009. Correlation of chlorophyll meter readings with gas exchange and chlorophyll fluorescence in flag leaves of rice (Oryza sativa L.) plants. Plant Production Science 12: 50-53.
Kumar S, Barpete S, Kumar J, Gupta P, and Sarker A, 2013. Global lentil production: constraints and strategies. SATSA Mukhapatra - Annu Technical Issue 17: 1-13.
Ladizinsky G and Hymowitz T, 1979. Seed protein electrophoresis in taxonomic and evolutionary studies. Theoretical and Applied Genetics 54: 145-151.
Merzlyak MN, Chivkunova OB, Solovchenko AE, and Razi Naqvi K, 2008. Light absorption by anthocyanins in juvenile, stressed, and senescing leaves. Journal of Experimental Botany 59(14): 3903-3911.
Nakano Y and Asada K, 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiology 22: 679-690.
Ozdemir S. 2002. Grain legume crops. Pp. 142. Hasad Publishing, Istanbul, Turkey.
Poormohammad Kiani S, Maury P, Sarrafi A, and Grieu P, 2008. QTL analysis of chlorophyll fluorescence parameters in sunflower (Helianthus annuus L.) under well-watered and water-stressed conditions. Plant Science 175(4): 565-573.
Raymond J, Rakariyatham N, and Azanza JL, 1993. Purification and some properties of polyphenol oxidase from sunflower seeds. Phytochemistry 34: 927-931.
Rihan HZ, Al–Issawi M, and Fuller MP, 2017. Advances in physiological and molecular aspects of plant cold tolerance. Journal of Plant Interactions 12(1):143-157.
Roháček K, Soukupová J, and Barták M, 2008. Chlorophyll fluorescence: a wonderful tool to study plant physiology and plant stress. In Schoefs B (ed.). Plant Cell Compartments- Selected Topics. Pp. 41-104. Research Signpost, Kerala, India.
Toor RK and Savage GP, 2005. Antioxidant activity in different fractions of tomatoes. Food Research International 38(5): 487-494. | ||
|
آمار تعداد مشاهده مقاله: 522 تعداد دریافت فایل اصل مقاله: 473 |
||