آمار
| تعداد نشریات | 45 |
| تعداد شمارهها | 1,469 |
| تعداد مقالات | 17,958 |
| تعداد مشاهده مقاله | 58,287,831 |
| تعداد دریافت فایل اصل مقاله | 19,747,703 |
Induced genes expression pattern in response to drought stress at seedling stage of wheat | ||
| Journal of Plant Physiology and Breeding | ||
| مقاله 9، دوره 9، شماره 1، شهریور 2019، صفحه 111-128 اصل مقاله (758.5 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2019.10388 | ||
| نویسندگان | ||
| Borzo Kazerani؛ Saeid Navabpour* | ||
| Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran. | ||
| چکیده | ||
| Molecular studies have shown that several genes with various functions are induced by drought stress and various transcription factors are involved in the regulation of the stress-inducible genes. To evaluate the effects of drought stress on gene expression, CRTBF2, DREB6 and DBF transcription factors as well as the expression pattern of Acyl, SuT5 and SuT4 genes were studied using qRT-PCR. The experiment was carried out as factorial based on completely randomized design. One factor included drought stress levels of -1, -2, -4 and -6 bar imposed by polyethylene glycol plus a control. The second factor consisted of four wheat cultivars as follows: Qaboos and Koohdasht (as drought tolerant), and Ehsan and Morvarid (as drought sensitive). Assessing the seedling morphological traits, such as germination percentage, plumule length and radicle length showed that with an increase in drought stress, the tolerant cultivars of Qaboos and Koohdasht showed lower decrease for these traits compared to the sensitive cultivars of Ehsan and Morvarid. The results of evaluating the gene expressions demonstrated that the DREB6 gene expression was higher in the tolerant cultivars of Qaboos and Koohdasht as compared to the sensitive cultivars of Ehsan and Morvarid, indicating the possible role of the product of this gene, as a transcription factor, in inducing promoters of drought tolerance genes and transcribing such genes. However, the CRTBF2 and DBF genes acted as repressor transcription factors in expressing the genes involved in the drought stress tolerance in the cultivars under study. The expressions of the Acyl and SuT5 genes were greater in the tolerant cultivars compared to the sensitive cultivars. The SuT4 gene expression was lower in both tolerant and sensitive cultivars than the expression of this gene in the corresponding control treatment. Therefore, it seems that the Acyl and SuT5 genes, through lipid decomposition and carbohydrate transport pathways, respectively, increased the tolerance of Qaboos and Koohdasht cultivars in this study. | ||
| کلیدواژهها | ||
| Drought stress؛ Gene expression؛ Seedling stage؛ Transcription factor؛ Wheat | ||
| مراجع | ||
|
Danyluk G and Sarhan F, 1990. Differential mRNA transcription during the induction of freezing tolerance in spring and winter wheat. Plant and Cell Physiology 31(5): 609-619.
Delessert C, Kazan K, Wilson LW, Van Der Straeten D, Manners J, Dennis ES and Dolferus R, 2005. The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis. The Plant Journal 43: 745-757.
De Paula FM, Pham Thi AT, Zuily-Fodil Y, Ferrari-Iliou R, da Silva V Jr and Mazliak P, 1993. Effects of water stress on the biosynthesis and degradation of polyunsaturated lipid molecular species in leaves of Vigna unguiculata. Plant Physiology and Biochemistry 31: 707-715.
El-Sharkawi HM and Springuel I, 1977. Germination of some crop plant seeds under reduced water potential. Seed Science and Technology 5: 677-688.
Gilmour SJ, Sebolt AM, Salazar MP, Everard JD and Thomashow MF, 2000. Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation. Plant Physiology 124(4):1854-1865.
GANRREC, 2018. A report of Golestan Agricultural and Natural Resources Research and Education Center. Retrieved December 17, 2018, from http://www.ganrrc.org.ir (In Persian).
Ibraheem O, 2011. Differential expression and regulation of sucrose transporters in rice (Oryza sativa L.) during environmental stress conditions. Ph.D. Thesis. Department of Biochemistry and Microbiology. University of Fort Hare. South Africa. 125p.
Kasuga M, Liu Q, Miura S, Yamaguchi-Shinozaki K and Shinozaki K, 1999. Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nature Biotechnology 17(3): 287-291.
Kizis D and Pages M, 2002. Maize DRE-binding proteins DBF1 and DBF2 are involved in rab17 regulation through drought-responsive element in an ABA-dependent pathway. The Plant Journal 30(6): 679-689.
Lata C and Prasad M, 2011. Role of DREBs in regulation of abiotic stress responses in plants. Journal of Experimental Botany 62(14): 4731-4748.
Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K and Shinozaki K, 1998. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. The Plant Cell 10: 1391-1406.
Michel BE and Kaufmann MR, 1974. The osmotic potential of polyethylen glycol 6000. Plant Physiology 51: 914-916.
Mizoi J, Shinozaki K and Yamaguchi-Shinozaki K, 2012. AP2/ERF family transcription factors in plant abiotic stress responses. Biochimica et Biophysica Acta 1819(2): 86-96.
Nakashima K, Shinwari ZK, Sakuma Y, Seki M, Miura S, Shinozaki K and Yamaguchi-Shinozaki K, 2000. Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression. Plant Molecular Biology 42(4): 657-665.
Navabpour S, Ramazanpour SS, Soltanloo H and Vakili Bastam S, 2015. Drought stress changed expression profile of some genes in tillering and pollination stages of adult wheat.Applied Science Reports 9(2): 100-109.
Okamuro JK, Caster B, Villarroel R, Van Montagu M and Jofuku DK, 1997. The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. PNAS USA 94(13): 7076-7081.
Payyavula RS, Tay KHC, Tsai CJ and Harding SA, 2011. The sucrose transporter family in Populus: the importance of a tonoplast PtaSUT4 to biomass and carbon partitioning. The Plant Journal 65(5): 757-770.
Pfaffl MW, Horgan GW and Dempfle L, 2002. Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research 30(9): e36. doi: 10.1093/nar/30.9.e36.
Prisco JT, Haddad CRB and Bastos JLP, 1992. Hydration-dehydration seed pre-treatment and its effects on seed germination under water stress conditions. Revista Brasileira de Botanica 15(1): 31-35.
Ramezanpour SS, 2007. Quantitative expression profile of wheat genes under cold stress. PhD Thesis. Faculty of Agricultural Sciences and Engineering. Department of Agronomy and Plant Breeding, Tehran University, Iran (In Persian with English abstract).
Ramezanpour SS, Charkazi F and Soltanloo H, 2010. Expression profile of some dehydration responsive element-binding factor under drought stress induced by PEG in wheat seedlings. American-Eurasian Journal of Agricultural and Environmental Sciences 7(5): 600-606.
Rauf M, Munir M, Ul-Hassan M, Ahmad M and Afzal M, 2007. Performance of wheat genotypes under osmotic stress at germination and early seedling growth stage. African Journal of Biotechnology 6(8): 971-975.
Shinozaki K and Yamaguchi-Shinozaki K, 2000. Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signalling pathways. Current Opinion in Plant Biology 3(3): 217-223.
Shoushi Dezfuli AA and Kalantar Ahmadi SA, 2018. Gene expression patterns of some transcription factors (MYB and WRKY) under salt stress in the seedling stage of alfalfa using qPCR. Journal of Crop Biotechnology 8(23): 27-41 (In Persian with English abstract).
Singh KB, Foley RC and Onate-Sanchez L, 2002. Transcription factors in plant defense and stress responses. Current Opinion in Plant Biology 5(5): 430-436.
Soltani A, Zeinali E, Galeshi S and Latifi N, 2001. Genetic variation for and interrelationship among seed vigor traits in wheat from the Caspian Sea coast of Iran. Seed Science and Technology 29(3): 653-662.
Vakili Bastam Sh, Ramezanpour SS, Soltanloo H, Zeinalinezhad Kh and Borzouei A, 2017. Study of drought tolerance in a wheat mutant line at the germination and adult stages. Environmental Stresses in Crop Sciences 10(1): 45-53 (In Persian with English abstract).
Weigel D, 1995.The APETALA2 domain is related to a novel type of DNA binding domain. The Plant Cell 7: 388-389.
Xu ZS, Ni ZY, Liu L, Nie LN, Li LC, Chen M and Ma YZ, 2008. Characterization of the TaAIDFa gene encoding a CRT/DRE-binding factor responsive to drought, high-salt, and cold stress in wheat. Molecular Genetics and Genomics 280(6): 497-508.
Yamaguchi-Shinozaki K and Shinozaki K, 1994. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. The Plant Cell 6: 251-264.
Yamaguchi-Shinozaki K and Shinozaki K, 2005. Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters. Trends in Plant Science 10(2): 88-94.
Zhang LH, Jia B, Zhuo RY, Liu JL, Pan HY, Baldwin TC and Zhang SH, 2012. An acyl-acyl carrier protein thioesterase gene isolated from wintersweet (Chimonanthus praecox), CpFATB, enhances drought tolerance in transgenic Tobacco (Nicotiana tobaccum).Plant Molecular Biology Reporter 30(2): 433-442. | ||
|
آمار تعداد مشاهده مقاله: 616 تعداد دریافت فایل اصل مقاله: 525 |
||