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Contamination control in Iranian seedless barberry micropropagation | ||
| Journal of Plant Physiology and Breeding | ||
| مقاله 8، دوره 9، شماره 1، شهریور 2019، صفحه 97-110 اصل مقاله (626.49 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2019.10387 | ||
| نویسندگان | ||
| Rahim Nazary Moghaddam Aghayeh1؛ Bahram Abedy* 1؛ Ahmad Balandari2؛ Leila Samiei3؛ Ali Tehranifar1 | ||
| 1Department of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 2Khorasan Science and Technology Park (KSTP), Mashhad, Iran. | ||
| 3Department of Ornamental Plants, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| چکیده | ||
| Iranian seedless barberry is a species with high degree of contamination in in vitro culture. The current study was aimed to investigate the effects of mercuric chloride (HgCl2), sodium hypochlorite (NaOCl) + HgCl2, HgCl2 + 8-hydroxyquinoline, cefotaxime + streptomycin, HgCl2 + shoot type, HgCl2 + explant position and HgCl2 + shoot color on the reduction of contamination. The results showed that the combined use of NaOCl and HgCl2 was more effective than their single use, but the contamination rate was relatively high. Interestingly, the results showed that explants sterilized with 100 mg/L HgCl2 and cultured in the medium containing 150 mg/L 8-hydroxyquinoline exhibited the lowest fungal contamination (15%), while those treated with 50 mg/L HgCl2 and cultured in the 8-hydroxyquinoline-free medium showed the highest fungal contamination (96%). Furthermore, treatment of 75 mg/Lcefotaxime + 70 mg/L streptomycin exhibited no bacterial contamination compared to the control with 58% bacterial contamination. Explants prepared from suckers and treated with 150 mg/L HgCl2 showed the lowest rates of fungal (16%) and bacterial (13%) contamination. The results also showed that explants collected from upper position had the lowest rates of fungal (20.87%) and bacterial (18.4%) infection. Explants taken from pinkish-colored shoots showed surprisingly the lowest rates of fungal contamination (13.33%), bacterial contamination (10%) and bud mortality (36.47%). In general, choice of explants from upper position of pinkish-colored suckers, sterilization of them with 100 mg/L HgCl2 for 5 min and their culture in medium containing 150 mg/L 8-hydroxyquinoline can be recommended for reducing the contamination. | ||
| کلیدواژه‌ها | ||
| 8-hydroxyquinoline؛ Bud؛ Contamination؛ Iranian seedless barberry؛ Mercury؛ Micropropagation | ||
| مراجع | ||
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