آمار
| تعداد نشریات | 45 |
| تعداد شمارهها | 1,435 |
| تعداد مقالات | 17,667 |
| تعداد مشاهده مقاله | 57,620,212 |
| تعداد دریافت فایل اصل مقاله | 19,317,229 |
Influence of plant growth regulators, carbohydrate source and concentration on micropropagation and other physiological traits of grape (Vitis vinifera L. cv. Shahroudi) under in vitro conditions | ||
| Journal of Plant Physiology and Breeding | ||
| مقاله 6، دوره 9، شماره 1، شهریور 2019، صفحه 75-82 اصل مقاله (634.08 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22034/jppb.2019.10378 | ||
| نویسندگان | ||
| AliReza Tarinejad* 1؛ Saleh Amiri2 | ||
| 1Department of Biotechnology, Faculty of Agriculture, Shahid Madani University, Tabriz, Iran | ||
| 2Academic Staff Member of Agricultural Biotechnology Research Institute of Iran (ABRII), Northwest and West Region, Tabriz, Iran. | ||
| چکیده | ||
| The grapevine (Vitis vinifera L.) is a native of central Asia and has been propagated by various methods, including in vitro propagation. Present research was conducted to study the effect of plant growth regulators (BAP, IBA), carbon sources (sucrose, glucose, fructose, table sugar) and their concentrations (30, 60 and 90 g/l) on the proliferation, root induction, protein, chlorophyll and carotenoid contents of the grape (Vitis vinifera L. cv. Shahroudi) in in vitro conditions based on a completely randomized design with three replications, using nodal explants. The result showed that the highest rate of proliferation (3.04 shoots per explant) occurred at MS medium, containing 1.5 mg/l BAP plus constant amounts of GA3 (0.3 mg/l) and IBA (0.1 mg/l). The highest rate of root induction (88.88%) was obtained at 1/2 MS medium at 0.5 mg/l IBA. Type and concentration of carbon source had a significant effect on some of the measured characteristics. The highest plantlet height was obtained in MS medium supplemented with 30 g/1 table sugar and sucrose. Also, the highest number of shoots per explant (3.5 shoots) belonged to the use of 30 g/l sucrose in the culture medium, followed by 30 g/l table sugar (3.23 shoots). The highest value of chlorophyll a was observed for 90 g/l glucose, followed by 30 g/l sucrose and 60 g/l table sugar. The highest chlorophyll b contentwas obtained for 60 g/l table sugar, followed by 30 g/l table sugar and sucrose. In general, with regard to most of the characteristics under study, it could be inferred that the propagation efficiency of 30 g/1 sucrose or table sugar was better than other carbon sources. Considering economic reasons and time, these treatments can be recommended for the commercial micropropagation of the Shahroudi cultivar of grapevine, instead of traditional methods of propagation. | ||
| کلیدواژهها | ||
| Grape؛ Growth regulators؛ Physiological traits؛ Pigments؛ Regeneration؛ Tissue culture | ||
| مراجع | ||
|
Abido AIA, Aly MAM, Hassanen SA and Rayan GA, 2013. In vitro propagation of grapevine (Vitis vinifera L.) Muscat of Alexandria cv. for conservation of endangerment. Middle-East Journal of Scientific Research 13: 328-337. Abou Rayya MS, Kassim NE and Ali EAM, 2011. Effect of different cytokinins concentrations and carbon sources on shoot proliferation of bitter almond nodal cuttings. Journal of American Science 6(9): 465-469. Abraham E, Hourton-Cabassa C, Erdei L and Szabados L, 2010. Methods for determination of proline in plants. In: Sunkar R. (eds). Plant Stress Tolerance. Methods in Molecular Biology (Methods and Protocols). Vol. 639. Pp. 317-331. Humana Press, USA. Alizadeh B and Tarinejad A, 2010. Application of MSTATC software in statistical analysis. Setoodeh Publications, Tabriz, Iran (In Persian). Alizadeh M, Singh SK and Patel VB, 2012. Comparative performance of in vitro multiplication in four grape (Vitis spp.) rootstock genotypes. International Journal of Plant Production 4(1): 41-50. Banilas G and Korkas E, 2007. Rapid micro-propagation of grapevine (cv. Agiorgitiko) through lateral bud development. e-Journal of Science & Technology 31-38. Barreto MS and Nookaraju A, 2007. Effect of auxin types on in vitro and ex vitro rooting and acclimatization of grapevine as influenced by substrates. Indian Journal of Horticulture 64(1): 5-11. Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1-2): 248-254. Chee R, Pool RM and Bucher D, 1984. A method for large scale in vitro propagation of Vitis. New York’s Food and Life Sciences Bulletin 109: 1-9. Gautheret RJ, 1955. The nutrition of plant tissue cultures. Annual Review of Plant Physiology 6: 433-484. Gopal J, Chamail A and Sarkar D, 2004. In vitro production of microtubers for conservation of potato germplasm: effect of genotype, abscisic acid, and sucrose. In Vitro Cellular & Developmental Biology-Plant 40: 485-490. Gray DJ and Benton CM, 1991. In vitro micropropagation and plant establishment of muscadine grape cultivars (Vitis rotundifolia). Plant Cell, Tissue and Organ Culture 27: 7-14. Heloir M-C, Fournioux J-C, Oziol L and Bessis R, 1997. An improved procedure for the propagation in vitro of grapevine (Vitis vinifera cv. Pinot noir) using axillary-bud microcuttings. Plant Cell, Tissue and Organ Culture 49: 223-225. Ilczuk A, Jagiełło-Kubiec K and Jacygrad E, 2013. The effect of carbon source in culture medium on micropropagation of common ninebark (Physocarpus opulifolius (L.) maxim.) ''Diable D''or''. Acta Scientiarum Polonorum, Hortorum Cultus 12(3): 23-33. Jaskani MJ, Abbas H, Sultana R, Khan MM, Qasim M and Khan IA, 2008. Effect of growth hormones on micropropagation of Vitis vinifera L. cv. Perlette. Pakistan Journal of Botany 40(1): 105-109. Kieber JJand Schaller GE, 2014. Cytokinins. Arabidopsis Book 12: e0168. doi: 10.1199/tab.0168. Lee N and Wetzstein HY, 1990. In vitro propagation of muscadine grape by axillary shoot proliferation. Journal of the American Society for Horticultural Science 115(2): 324-329. Lewandowski VT, 1991. Rooting and acclimatization of micropropagated Vitis labrusca Delaware. HortScience 26(5): 586-589. Li Y, He N, Hou J, Xu L, Liu C, Zhang J, Wang Q, Zhang X and Wu X, 2018. Factors influencing leaf chlorophyll content in natural forests at the biome scale. Frontiers in Ecology and Evolution 6: 64. doi: 10.3389/fevo.2018.00064. Lichtenthaler HK and Wellburn AR, 1983. Determination of total carotenoids and chlorophylls a and b in leaf extracts in different solvents. Biochemical Society Transactions 11(5): 591-592. Mamiya K and Sakamoto Y, 2000. Effects of sugar concentration and strength of basal medium on conversion of somatic embryos in Asparagus officinalis L. Scientia Horticulturae 84(1-2): 15-26. Murashige T and Skoog F, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15(3): 473-497.
Singh SK, Khawale RN and Singh SP, 2004. Technique for rapid in vitro multiplication of Vitis vinifera L. cultivars. The Journal of Horticultural Science and Biotechnology 79(2): 267-272. Swamy MK, Sudipta KM, Balasubramanya S and Anuradha M, 2010. Effect of different carbon sources on in vitro morphogenetic response of patchouli (Pogostemon cablin Benth.). Journal of Phytology 2(8): 11-17. Talcott ST, Hernandez-Brenes C, Pires DM and Del Pozo-Insfran D, 2003. Phytochemical stability and color retention of copigmented and processed muscadine grape juice. Journal of Agricultural and Food Chemistry 51(4): 957-963. Tarinejad A, 2013. Effects of disinfectants and antibiotics on contamination during propagation of walnut (Juglans regia L.). Research on Crops 14(1): 219-225. Yaseen M, Ahmad T, Sablok G, Standardi A and Hafiz IA, 2013. Review: role of carbon sources for in vitro plant growth and development. Molecular Biology Reports 40(4): 2837-2849. | ||
|
آمار تعداد مشاهده مقاله: 612 تعداد دریافت فایل اصل مقاله: 598 |
||